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Keywords = TLCV

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15 pages, 27136 KiB  
Article
Identification of Crucial Amino Acids in Begomovirus C4 Proteins Involved in the Modulation of the Severity of Leaf Curling Symptoms
by Kao-Wei Dai, Yu-Ting Tsai, Chia-Ying Wu, Yi-Chin Lai, Na-Sheng Lin and Chung-Chi Hu
Viruses 2022, 14(3), 499; https://doi.org/10.3390/v14030499 - 28 Feb 2022
Cited by 14 | Viewed by 3803
Abstract
Begomoviruses frequently inflict upward or downward leaf curling symptoms on infected plants, leading to severe economic damages. Knowledge of the underlying mechanism controlling the leaf curling severity may facilitate the development of alternative disease management strategies. In this study, through genomic recombination between [...] Read more.
Begomoviruses frequently inflict upward or downward leaf curling symptoms on infected plants, leading to severe economic damages. Knowledge of the underlying mechanism controlling the leaf curling severity may facilitate the development of alternative disease management strategies. In this study, through genomic recombination between Ageratum yellow vein virus Nan-Tou strain (AYVV-NT) and Tomato leaf curl virus Tai-Chung Strain (TLCV-TC), which caused upward and downward leaf curling on Nicotiana benthamiana, respectively, it was found that the coding region of C4 protein might be involved in the determination of leaf curling directions. Sequence comparison and mutational analysis revealed that the cysteine and glycine at position 8 and 14 of AYVV-TC C4 protein, respectively, are involved in the modulation of leaf curling symptoms. Cross-protection assays further demonstrated that N. benthamiana inoculated with AYVV-carrying mutations of the aforementioned amino acids exhibited attenuated leaf curling symptoms under the challenge of wild-type AYVV-NT. Together, these findings revealed a new function of begomovirus C4 proteins involved in the modulation of leaf curling severity during symptom formation and suggested potential applications for managing viral diseases through manipulating the symptoms. Full article
(This article belongs to the Special Issue State-of-the-Art Plant-Virus Interactions in Asia)
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15 pages, 2963 KiB  
Article
Diallel Analysis for Morphological and Biochemical Traits in Tomato Cultivated under the Influence of Tomato Leaf Curl Virus
by Prashant Kaushik and Major Singh Dhaliwal
Agronomy 2018, 8(8), 153; https://doi.org/10.3390/agronomy8080153 - 19 Aug 2018
Cited by 27 | Viewed by 8551
Abstract
Eloquent information about the genetic basis of inheritance is important for any breeding program. Therefore, a diallel study was conducted under the influence of tomato leaf curl virus (TLCV) disease, using the eleven advanced lines of tomato. Firstly, information regarding percent disease index [...] Read more.
Eloquent information about the genetic basis of inheritance is important for any breeding program. Therefore, a diallel study was conducted under the influence of tomato leaf curl virus (TLCV) disease, using the eleven advanced lines of tomato. Firstly, information regarding percent disease index (PDI) was determined via artificial screening with viruliferous whiteflies. Later, these lines were crossed in a half diallel mating design to produce fifty-five one-way hybrids. These hybrids and parental genotypes were evaluated for morphological and biochemical traits under open field conditions. Using the Griffing approach (Method II and Model I), the basis of the inheritance of traits was determined. Furthermore, a Bayesian model was applied to the total yield descriptor. Correlation data indicated that total yield was not correlated with any other trait. The significant general combining ability (GCA) and specific combining ability (SCA) values indicate exploitable genetic variation. The broad-sense heritability values were larger than narrow-sense heritability, showing that selection will be efficient for the improvement of these traits. Hybrid combinations H23, H42 and H49 can be considered efficient for the selection of multiple traits, including yield. Overall, this study provides useful information regarding the genetics of important traits of tomato under TLCV infestation. Full article
(This article belongs to the Special Issue Genetics and Genomics of Tomato and Solanaceae)
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