Search Results (6)

Future Artemis-class missions to Mars will expose astronauts to prolonged space radiation (SR), sleep disruption, and operational demands requiring greater autonomy, placing decision making and executive function at heightened risk. Both SR and sleep fragmentation (SF) independently impair cognition, yet their combined effects remain poorly understood. Using the Associative Recognition Memory and Interference (ARMIT) task, we assessed cognitive performance in male rats exposed to 10 cGy of Galactic Cosmic Ray simulation (GCRsim), SF, or both. Under well-rested conditions, GCRsim-exposed rats exhibited overt deficits in the C.1.2 stage, performing at chance when reinforcement contingencies shifted, consistent with impaired cognitive flexibility. In contrast, high-performing GCRsim-exposed rats that initially performed comparably to Sham s revealed latent deficits following a single night of SF. Specifically, the SF-induced loss of C.1.3 performance was accompanied by perseverative errors (persistently selecting outdated cues despite negative feedback), reflecting impaired attentional control and decision updating. Sham s maintained stable performance after SF. These findings support a two-hit vulnerability model in which SR primes corticostriatal and frontoparietal networks for collapse under subsequent sleep disruption. Operationally, this suggests that astronauts may display either persistent or stress-induced deficits, with both modes threatening mission success. Identifying mechanisms of such vulnerabilities is essential for countermeasure development. Full article

This study investigated the changes in the acoustic emission (AE) activity emitted in fiber-reinforced concrete (FRC) under flexure at two temperatures (25 °C and −20 °C). Seven concrete mixtures were developed with different water-binder ratios (w/b) (0.4 and 0.55), different fiber materials (steel fiber (SF) and synthetic polypropylene fiber (Syn-PF)), different fiber lengths (19 mm and 38 mm), and various Syn-PF contents (0%, 0.2%, and 1%). Prisms with dimensions of 100 × 100 × 400 mm from each mixture underwent a four-point monotonic flexure load while collecting the emitted acoustic waves via attached AE sensors. AE parameter-based analyses, including b-value, improved b-value (Ib-value), intensity, and rise time/average signal amplitude (RA) analyses, were performed using the raw AE data to highlight the change in the AE activity associated with different stages of damage (micro- and macro-cracking). The results showed that the number of hits, average frequency, cumulative signal strength (CSS), and energy were higher for the waves released at −20 °C compared to those obtained at 25 °C. The onset of the first visible micro- and macro-cracks was noticed to be associated with a significant spike in CSS, historic index (H (t)), severity (S_r) curves, a noticeable dip in the b-value curve, and a compression in bellows/fluctuations of the Ib-value curve for both testing temperatures. In addition, time and load thresholds of micro- and macro-cracks increased when samples were cooled down and tested at −20 °C, especially in the mixtures with higher w/b, longer fibers, and lower fiber content. This improvement in mechanical performance and cracking threshold limits was associated with higher AE activity in terms of an overall increase in CSS, S_r, and H (t) values and an overall reduction in b-values. In addition, varying the concrete mixture design parameters, including the w/b ratio as well as fiber type, content, and length, showed a significant impact on the flexural behavior and the AE activity of the tested mixtures at both temperatures (25 °C and −20 °C). Intensity and RA analysis parameters allowed the development of two charts to characterize the detected AE events, whether associated with micro- and macro-cracks considering the temperature effect. Full article

Ultra-high-performance concrete with coarse aggregates (UHPC-CA) has the advantages of high strength, strong shrinkage resistance and a lower production cost, presenting a broad application prospect in civil engineering construction. In view of the difficulty in establishing a mathematical model to accurately predict the mechanical properties of UHPC-CA, the back-propagation artificial neural network (BP-ANN) method is used to fully consider the various influential factors of the compressive strength (CS) and flexural strength (FS) of UHPC-CA in this paper. By taking the content of cement (C), silica fume (SF), slag, fly ash (FA), coarse aggregate (CA), steel fiber, the water–binder ratio (w/b), the sand rate (SR), the cement type (CT), and the curing method (CM) as input variables, and the CS and FS of UHPC-CA as output objectives, the BP-ANN model with three layers has been well-trained, validated and tested with 220 experimental data in the studies published in the literature. Four evaluating indicators including the determination coefficient (R²), the root mean square error (RMSE), the mean absolute percentage error (MAPE), and the integral absolute error (IAE) were used to evaluate the prediction accuracy of the BP-ANN model. A parametric study for the various influential factors on the CS and FS of UHPC-CA was conducted using the BP-ANN model and the corresponding influential mechanisms were analyzed. Finally, the inclusion levels for the CA, steel fiber, and the dimensionless parameters of the W/B and sand rate were recommended to obtain the optimal strength of UHPC-CA. Full article

Bacillus thuringiensis Vip3Aa has been widely used in transgenic crops to resist the erosion of insects. The Scavenger Receptor-C (SR-C) and Fibroblast Growth Factor Receptor (FGFR) of Spodoptera frugiperda (Sf-SR-C and Sf-FGFR) have formerly been identified as the cell receptors of Vip3Aa. However, the interaction mechanism of Vip3Aa binding to Sf-SR-C or Sf-FGFR is still unknown. Here, we purified the MAM domain of Sf-SR-C (Sf-MAM) and the Sf-FGFR ectodomain expressed extracellularly by Sf9 cells. We then solved the crystal structure of the Sf-MAM domain. Structure docking analysis of the Sf-MAM and Vip3Aa C-terminal domain (CTD) excluded the possibility of the two proteins binding. A further surface plasmon resonance (SPR) assay also revealed that the Sf-MAM and Sf-FGFR ectodomain could not bind to the Vip3Aa protein. Our results have raised the urgency of determining the authentic cell receptor for Vip3Aa. Full article

Vip3Aa is a novel insecticidal protein secreted by Bacillus thuringiensis (Bt) during its vegetative growth stages. It has high insecticidal activity against lepidopteran pests such as Spodoptera frugiperda, and has no cross-resistance with Cry insecticidal proteins. As a new type of insecticide, it plays an important role in controlling agricultural pests. However, the insecticidal mechanism of the Vip3Aa toxin, especially its definite receptors, have not been fully revealed. In this study, the previously reported Vip3Aa receptor genes Sf-FGFR and Sf-SR-C were knocked out separately using the CRISPR/Cas9 system. Bioassay results showed that the sensitivity of these two knockout strains to Vip3Aa were not significantly changed compared to that of the normal strain. The current results are not consistent with the previously reports that Sf-SR-C and Sf-FGFR were the receptors of Vip3Aa in vitro. This suggests that the Sf-SR-C and Sf-FGFR genes we tested may not be critical in the mode of action of Vip3Aa in vivo in Spodoptera frugiperda. Full article

The combinatorial library of novel potential anticancer agents, namely, 2-(аlkyl-, alkaryl-, aryl-, hetaryl-)[1,2,4]triazolo[1,5-c]quinazolines, was synthesized by the heterocyclization of the аlkyl-, alkaryl-, aryl-, hetarylcarboxylic acid (3Н-quinazoline-4-ylidene)hydrazides by oxidative heterocyclization of the 4-(arylidenehydrazino)quinazolines using bromine, and by the heterocyclization of N-(2-cyanophenyl)formimidic acid ethyl ester. The optimal method for synthesis of the s-triazolo[1,5-c]quinazolines appeared to be cyclocondensation of the corresponding carboxylic acid (3H-quinazoline-4-ylidene)hydrazides. The compounds’ structures were established by 1H, 13C NMR, LC- and EI-MS analysis. The in vitro screening of anticancer activity determined the most active compound to be 3,4,5-trimethoxy-N'-[quinazolin-4(3H)-ylidene]benzohydrazide (3.20) in micromolar concentrations with the GI50 level (MG_MID, GI50 is 2.29). Thus, the cancer cell lines whose growth is greatly inhibited by compound 3.20 are: non-small cell lung cancer (NCI-H522, GI50=0.34), CNS (SF-295, GI50=0.95), ovarian (OVCAR-3, GI50=0.33), prostate (PC-3, GI50=0.56), and breast cancer (MCF7, GI50=0.52), leukemia (K-562, GI50=0.41; SR, GI50=0.29), and melanoma (MDA-MB-435, GI50=0.31; SK-MEL-5, GI50=0.74; UACC-62, GI50=0.32). SAR-analysis is also discussed. Full article