Search Results (2)

Paper presents an effective technique for developing reduced-order models to predict the dynamic responses of systems using the receptance coupling and frequency-based substructuring (RCFBS) method. The proposed approach is particularly suited for reconfigurable dynamic systems across various applications, like cars, robots, mechanical machineries, and aerospace structures. The methodology focuses on determining the overall system receptance matrix by coupling the receptance matrices (FRFs) of individual subsystems in a disassembled configuration. Two case studies, one with distributed parameters and the other with lumped parameters, are used to illustrate the application of this approach. The first case involves coupling three substructures with flexible components under fixed–fixed boundary conditions, while the second case examines the coupling of subsystems characterized by multiple masses, springs, and dampers, with various internal and connection degrees of freedom. The accuracy of the proposed method is validated against a numerical finite element analysis (FEA), direct methods, and a modal analysis. The results demonstrate the reliability of RCFBS in predicting dynamic responses for reconfigurable systems, offering an efficient framework for reduced-order modeling by focusing on critical points of interest without the need to account for detailed modeling with numerous degrees of freedom. Full article

Regulons, which serve as co-regulated gene groups contributing to the transcriptional regulation of microbial genomes, have the potential to aid in understanding of underlying regulatory mechanisms. In this study, we designed a novel computational pipeline, regulon identification based on comparative genomics and transcriptomics analysis (RECTA), for regulon prediction related to the gene regulatory network under certain conditions. To demonstrate the effectiveness of this tool, we implemented RECTA on Lactococcus lactis MG1363 data to elucidate acid-response regulons. A total of 51 regulons were identified, 14 of which have computational-verified significance. Among these 14 regulons, five of them were computationally predicted to be connected with acid stress response. Validated by literature, 33 genes in Lactococcus lactis MG1363 were found to have orthologous genes which were associated with six regulons. An acid response related regulatory network was constructed, involving two trans-membrane proteins, eight regulons (llrA, llrC, hllA, ccpA, NHP6A, rcfB, regulons #8 and #39), nine functional modules, and 33 genes with orthologous genes known to be associated with acid stress. The predicted response pathways could serve as promising candidates for better acid tolerance engineering in Lactococcus lactis. Our RECTA pipeline provides an effective way to construct a reliable gene regulatory network through regulon elucidation, and has strong application power and can be effectively applied to other bacterial genomes where the elucidation of the transcriptional regulation network is needed. Full article