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Keywords = PxTreh2

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13 pages, 2543 KiB  
Article
C/EBPα Regulates PxTreh1 and PxTreh2 Trehalase-Related Bt Resistance in Plutella xylostella (L.)
by Jia Liu, Zheming Liu, Haihao Ma, Yaying Yu, Chengjia Zhang, Wei Zheng, Yilong Man, Hang Zhu, Yong Zhou, Xi Chen, Xiaomao Zhou and Aiping Zeng
Insects 2022, 13(4), 340; https://doi.org/10.3390/insects13040340 - 30 Mar 2022
Cited by 6 | Viewed by 2433
Abstract
Trehalase regulates energy metabolism in insects by converting trehalose into two glucose molecules. High amounts of trehalase are critical for insect flight and larval stress resistance. However, whether trehalase participates in the development of pesticide resistance remains unclear. In this study, we explored [...] Read more.
Trehalase regulates energy metabolism in insects by converting trehalose into two glucose molecules. High amounts of trehalase are critical for insect flight and larval stress resistance. However, whether trehalase participates in the development of pesticide resistance remains unclear. In this study, we explored this phenomenon and the mechanism that underlies the regulation of Trehalase transcription. We found that overexpression of PxTreh1 and PxTreh2 induced Bacillus thuringiensis (Bt) resistance in Plutella xylostella. The promoter sequences of PxTreh1 and PxTreh2 were also cloned and identified. The dual-luciferase reporter system and RNA interference technology revealed that the expression of PxTreh1 and PxTreh2 genes is possibly regulated by the CCAAT enhancer-binding protein (C/EBPα). A yeast one-hybrid experiment confirmed the interaction between C/EBPα and the PxTreh2 promoter. The findings of this study suggest that C/EBPα mediates the adaptability of P. xylostella to adverse environmental stressors by regulating the expression of trehalase. Full article
(This article belongs to the Section Insect Molecular Biology and Genomics)
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