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Pontechium maculatum (Boraginaceae) is a species of high conservation concern in the Romanian flora. It is assigned the national IUCN category “vulnerable”, legally protected according to the national Biological Diversity Act, and listed in Annex II of the Habitats Directive (Council Directive 92/43/EEC). P. maculatum, formerly known as Echium russicum, is a biennial herbaceous plant, rare in many parts of its range and even critically endangered in some countries. In Romania, populations of this species are found in several areas of Transylvania, Moldova, Dobrogea, and Oltenia, mostly within protected areas, with the number of individuals reaching over 1500, and the populations being stable with a favorable conservation status. In the present study, ten populations of P. maculatum from the “Iron Gates” Natural Park in SW Romania were analyzed. For each density, the composition of the plant community was assessed, as well as some morphological and physiological parameters of the plants. The results indicated the phytocoenotic variability of the species, with a different dominant abundance in the floristic composition of several plant communities. Statistical analysis of quantitative traits revealed variability depending on density (at lower densities, plants have a higher number of leaves and photosynthetic rate). Also, the photosynthetic rate was mainly temperature-dependent, rising with this factor. This research found that in some areas, there are insufficient numbers of individuals due to invasive species. The identified variability of Pontechium maculatum in the “Iron Gates” Natural Park is demonstrated by the interplay of ecological factors, human influence, and conservation efforts. Our findings suggest that effective conservation strategies should focus on managing invasive species and enhancing habitat conditions to support P. maculatum populations. Full article

Pontechium maculatum, a species of ornamental, apicultural, health and medicinal value, is threatened in some Central European countries including Poland. Its propagation using seeds or in vitro techniques is needed for multiple applications including conservation. Generative propagation efficacy of P. maculatum plants representing different genetic resources (received from botanical gardens in Germany and in Poland) propagated from seeds or in tissue culture was assessed. Moreover, an efficient technique of propagation of P. maculatum using in vitro shoot culture from seedlings was elaborated for the first time. The highest propagation efficacy was noted for German plants of seed origin. The ability of seeds to germinate was similar for all plants; however, seeds were in a state of dormancy, which was broken by GA₃. After two years of storage, the seeds still retained the ability to germinate though seeds from propagated in vitro plants germinated more poorly than those from seed-originated plants. The ploidy assessment showed that some in vitro-origin plants had altered DNA content. The results indicate that efficacy of generative propagation of P. maculatum is resource dependent. Furthermore, results suggest that cultivation in vitro influenced some generative features of examined species, which makes this way of P. maculatum propagation a valuable source of genetic variation and a potential breeding tool. Full article