The classification of carbapenemases can help guide therapy. The present study evaluated the performance of the CPO detection test, included in the BD Phoenix
™ NMIC-501 panel for the detection and classification of carbapenemases on the representative molecularly characterized strains collection from Mexico.
[...] Read more.
The classification of carbapenemases can help guide therapy. The present study evaluated the performance of the CPO detection test, included in the BD Phoenix
™ NMIC-501 panel for the detection and classification of carbapenemases on the representative molecularly characterized strains collection from Mexico. Carbapenem non-susceptible isolates collected in Mexico were included. The clinical isolates (
n = 484) comprised
Klebsiella pneumoniae (
n = 154),
Escherichia coli (
n = 150), and
P. aeruginosa (
n = 180). BD Phoenix CPO NMIC-504 and NMIC-501 panels were used for the identification of species, antimicrobial susceptibility tests, and detection of CPOs. For the detection of carbapenemase-encoding genes,
E. coli and
K. pneumoniae were evaluated using PCR assays for
blaNDM-1,
blaKPC,
blaVIM,
blaIMP, and
blaOXA-48-like. For
P. aeruginosa,
blaVIM,
blaIMP, and
blaGES were detected using PCR. Regarding
E. coli, the CPO panels had a sensitivity of 70% and specificity of 83.33% for the detection of a class B carbapenemase (
blaNDM in the molecular test). Regarding
K. pneumoniae, the panels had a sensitivity of 75% and specificity of 100% for the detection of a class A carbapenemase (
blaKPC in the molecular test). The Phoenix NMIC-501 panels are reliable for detecting class B carbapenemases in
E. coli. The carbapenemase classification in
K. pneumoniae for class A carbapenemases has a high specificity and PPV; thus, a positive result is of high value.
Full article
