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American foulbrood is a dangerous bee disease that attacks the sealed brood. It quickly leads to the death of bee colonies. Efficient diagnosis of this disease is essential. As specific odours are produced when larvae rot, it was investigated whether an electronic nose can distinguish between colonies affected by American foulbrood and healthy ones. The experiment was conducted in an apiary with 18 bee families, 9 of which showed symptoms of the disease confirmed by laboratory diagnostics. Three units of the Beesensor V.2 device based on an array of six semiconductor TGS gas sensors, manufactured by Figaro, were tested. Each copy of the device was tested in all bee colonies: sick and healthy. The measurement session per bee colony lasted 40 min and yielded results from four 10 min measurements. One 10-min measurement consisted of a 5 min regeneration phase and a 5 min object-measurement phase. For the experiments, we used both classical classification methods such as k-nearest neighbour, Naive Bayes, Support Vector Machine, discretized logistic regression, random forests, and committee of classifiers, that is, methods based on extracted representative data fragments. We also used methods based on the entire 600 s series, in this study of sequential neural networks. We considered, in this study, six options for data preparation as part of the transformation of data series into representative results. Among others, we used single stabilised sensor readings as well as average values from stable areas. For verifying the quality of the classical classifiers, we used the 25-fold train-and-test method. The effectiveness of the tested methods reached a threshold of 75 per cent, with results stable between 65 and 70 per cent. As an element to confirm the possibility of class separation using an artificial nose, we used applied visualisations of classes. It is clear from the experiments conducted that the artificial nose tested has practical potential. Our experiments show that the approach to the problem under study by sequential network learning on a sequence of data is comparable to the best classical methods based on discrete data samples. The results of the experiment showed that the Beesensor V.2 along with properly selected classification techniques can become a tool to facilitate rapid diagnosis of American foulbrood under field conditions. Full article

American foulbrood is a dangerous disease of bee broods found worldwide, caused by the Paenibacillus larvae larvae L. bacterium. In an experiment, the possibility of detecting colonies of this bacterium on MYPGP substrates (which contains yeast extract, Mueller-Hinton broth, glucose, K2HPO4, sodium pyruvate, and agar) was tested using a prototype of a multi-sensor recorder of the MCA-8 sensor signal with a matrix of six semiconductors: TGS 823, TGS 826, TGS 832, TGS 2600, TGS 2602, and TGS 2603 from Figaro. Two twin prototypes of the MCA-8 measurement device, M1 and M2, were used in the study. Each prototype was attached to two laboratory test chambers: a wooden one and a polystyrene one. For the experiment, the strain used was P. l. larvae ATCC 9545, ERIC I. On MYPGP medium, often used for laboratory diagnosis of American foulbrood, this bacterium produces small, transparent, smooth, and shiny colonies. Gas samples from over culture media of one- and two-day-old foulbrood P. l. larvae (with no colonies visible to the naked eye) and from over culture media older than 2 days (with visible bacterial colonies) were examined. In addition, the air from empty chambers was tested. The measurement time was 20 min, including a 10-min testing exposure phase and a 10-min sensor regeneration phase. The results were analyzed in two variants: without baseline correction and with baseline correction. We tested 14 classifiers and found that a prototype of a multi-sensor recorder of the MCA-8 sensor signal was capable of detecting colonies of P. l. larvae on MYPGP substrate with a 97% efficiency and could distinguish between MYPGP substrates with 1–2 days of culture, and substrates with older cultures. The efficacy of copies of the prototypes M1 and M2 was shown to differ slightly. The weighted method with Canberra metrics (Canberra.811) and kNN with Canberra and Manhattan metrics (Canberra. 1nn and manhattan.1nn) proved to be the most effective classifiers. Full article