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Article

1,878 Views

11 Pages

Integrating PLOR and SPAAC Click Chemistry for Efficient Site-Specific Fluorescent Labeling of RNA

Yanyan Xue,
Xiao Si,
Daxu Yin,
Shengzhe Zhang and
Hua Dai

Int. J. Mol. Sci.2025, 26(6), 2601;https://doi.org/10.3390/ijms26062601

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13 March 2025

Precisely fluorescently labeling specific nucleotide sites of RNA is critical for gaining insights into the structure and function of RNA through multiple fluorescence detection techniques. The position-selective labeling of RNA (PLOR) method provide...

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Article

1 Citations

3,236 Views

11 Pages

Quantitative Analysis of Transcription Termination via Position-Selective Labeling of RNA (PLOR) Method

Ping-Yi Chien,
Lingzhi Gao and
Yu Liu

Int. J. Mol. Sci.2023, 24(5), 4934;https://doi.org/10.3390/ijms24054934

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3 March 2023

T7 RNA polymerase is the most widely used enzyme in RNA synthesis, and it is also used for RNA labeling in position-selective labeling of RNA (PLOR). PLOR is a liquid–solid hybrid phase method that has been developed to introduce labels to spec...

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Article

1,669 Views

14 Pages

Observation of SAM-VI Riboswitch Dynamics Using Single-Molecule FRET

Yanyan Xue,
Yi Sun,
Yichun Xia,
Xiuming Liu and
Hua Dai

Biomolecules2025, 15(6), 841;https://doi.org/10.3390/biom15060841

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9 June 2025

Riboswitches regulate gene expression through intricate dynamic conformational transitions, with divalent cation Mg²⁺ and their ligands playing pivotal roles in this process. The dynamic structural mechanism by which the S-adenosyl-L-methionine (SAM)...

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