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Keywords = IncI1α conjugative plasmid

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25 pages, 3662 KB  
Article
Deciphering the Causes of IbfA-Mediated Abortive Infection in the P22-like Phage UAB_Phi20
by Júlia López-Pérez, Pilar Cortés, Susana Campoy, Ivan Erill and Montserrat Llagostera
Int. J. Mol. Sci. 2025, 26(10), 4918; https://doi.org/10.3390/ijms26104918 - 20 May 2025
Cited by 1 | Viewed by 2351
Abstract
The study of bacterial defense mechanisms against phages is becoming increasingly relevant due to their impact on the effectiveness of phage therapy. Employing a multifaceted approach that combines bioinformatics, molecular microbiology, TEM microscopy, and conventional microbiology techniques, here, we identify the ibfA gene [...] Read more.
The study of bacterial defense mechanisms against phages is becoming increasingly relevant due to their impact on the effectiveness of phage therapy. Employing a multifaceted approach that combines bioinformatics, molecular microbiology, TEM microscopy, and conventional microbiology techniques, here, we identify the ibfA gene as a novel defense factor targeting the virulent phage UAB_Phi20, acquired by Salmonella Typhimurium through lateral transfer on the IncI1α conjugative plasmid pUA1135 after oral phage therapy in broilers. IbfA, a two-domain protein containing ATPase and TOPRIM domains, significantly reduces UAB_Phi20 productivity, as indicated by decreased EOP, ECOI, and a diminished burst size, potentially reducing cellular viability without causing observable lysis. Our results indicate that IbfA enhances the transcription of early genes, including the antirepressor ant, which inhibits the C2 repressor of the lytic cycle. This may cause an imbalance in Cro/C2 concentration, leading to the observed reduction in the transcription of late genes encoding structural and cellular lysis proteins, and resulting in the abortion of UAB_Phi20 infection. Full article
(This article belongs to the Special Issue Exploring Phage–Host Interactions: Novel Findings and Perspectives)
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