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Keywords = EPPO quarantine regulation

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13 pages, 5539 KiB  
Article
Molecular Diagnostics in Tomato: Chip Digital PCR Assays Targeted to Identify and Quantify Clavibacter michiganensis subsp. michiganensis and Ralstonia solanacearum in planta
by Caterina Morcia, Isabella Piazza, Roberta Ghizzoni, Valeria Terzi, Ilaria Carrara, Giovanni Bolli and Giorgio Chiusa
Horticulturae 2023, 9(5), 553; https://doi.org/10.3390/horticulturae9050553 - 4 May 2023
Cited by 7 | Viewed by 2648
Abstract
Clavibacter michiganensis subsp. michiganensis (Cmm) and Ralstonia solanacearum (Rs) are important bacterial pathogens of tomatoes (Solanum lycopersicum), are included in A2 list in the EPPO (European and Mediterranean Plant Protection Organization) region and are recommended for regulation as quarantine pests. The [...] Read more.
Clavibacter michiganensis subsp. michiganensis (Cmm) and Ralstonia solanacearum (Rs) are important bacterial pathogens of tomatoes (Solanum lycopersicum), are included in A2 list in the EPPO (European and Mediterranean Plant Protection Organization) region and are recommended for regulation as quarantine pests. The control of quarantine pathogens requires accurate and rapid detection tools. In this study, a method based on chip digital PCR (cdPCR) was developed to identify and quantify Cmm and Rs. The assays were tested on pure bacteria samples and on tomato samples naturally contaminated or spiked with bacteria DNA. For a better estimation of infection level in host plants, duplex assays that are able to simultaneously amplify plant and bacteria DNA were developed. The two cdPCR assays proposed can be used for the rapid and timely detection of this group of high-risk quarantine bacteria to prevent the spread of pathogens and the occurrence of disease in other areas. Full article
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27 pages, 18803 KiB  
Review
Exotic and Emergent Citrus Viruses Relevant to the Mediterranean Region
by Antonino F. Catara, Moshe Bar-Joseph and Grazia Licciardello
Agriculture 2021, 11(9), 839; https://doi.org/10.3390/agriculture11090839 - 31 Aug 2021
Cited by 7 | Viewed by 11754
Abstract
Citrus production in the Mediterranean area is of considerable importance, in both cultural and economic terms, and the viability of the industry greatly depends on proper phytosanitary management. In this review, we focus on exotic and emerging dangerous citrus viruses that have still [...] Read more.
Citrus production in the Mediterranean area is of considerable importance, in both cultural and economic terms, and the viability of the industry greatly depends on proper phytosanitary management. In this review, we focus on exotic and emerging dangerous citrus viruses that have still not been reported in the countries of the Mediterranean area, that are not yet regulated or that are restricted to certain small areas. We also discuss the contribution that old and new technologies may offer for valuable surveys aimed at promoting the adoption and sharing of better control measures and the production of pathogen-tested citrus trees and rootstocks. Full article
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