Search Results (3)

Recently, there has been a growing effort to reduce the environmental impact of products throughout their life cycle, particularly during the end-of-life (EoL) stage. To incentivise consumers’ recycling/reusing behaviours and enhance their environmental awareness, a novel ICT-based system for recycling and eco-shopping has been developed in this paper. The recycling of EoL products is conducted based on information-communication technologies to remotely monitor and manage the recycled products (such as electronics or household bio-wastes), enabling consumers’ recycling process over the Internet. Consumers are awarded the eco-credits, which can be used for various forms of eco-incentives, such as shopping discounts, tree planting donations, and exchanges for theatre and museum tickets. The eco-costs reflect the environmental impact of a product throughout its life cycle. The consumer is informed about the eco-costs through eco-shopping, which are displayed on a payment receipt. Both eco-costs and eco-credits are recorded in the consumer’s eco-account. To develop the recycling and eco-shopping system, multiple information-communication technologies are utilised, such as hardware digital monitoring/control, Internet-based communication services, traceability media (bar-code and QR code), user identity recognition and privacy protection, and multi-language supports. A case study is conducted, including online tracking of the recycling process and then implementing incentive activities with the eco-credits and eco-costs. The system has been successfully validated via illustrating recycling, eco-shopping, and eco-incentives in public places (e.g., schools, urban cultural centres), as well as promoting the consumer’s participation in recycling and enhancing their environmental awareness, which proved the successful implementation of the novel contribution of this research. Full article

Microbial biofilm systems are defined as interface-associated microorganisms embedded into a self-produced matrix. The extracellular matrix represents a continuous challenge in terms of characterization and analysis. The tools applied in more detailed studies comprise extraction/chemical analysis, molecular characterization, and visualisation using various techniques. Imaging by laser microscopy became a standard tool for biofilm analysis, and, in combination with fluorescently labelled lectins, the glycoconjugates of the matrix can be assessed. By employing this approach a wide range of pure culture biofilms from different habitats were examined using the commercially available lectins. From the results, a binary barcode pattern of lectin binding can be generated. Furthermore, the results can be fine-tuned and transferred into a heat map according to signal intensity. The lectin barcode approach is suggested as a useful tool for investigating the biofilm matrix characteristics and dynamics at various levels, e.g. bacterial cell surfaces, adhesive footprints, individual microcolonies, and the gross biofilm or bio-aggregate. Hence fluorescence lectin bar-coding (FLBC) serves as a basis for a subsequent tailor-made fluorescence lectin-binding analysis (FLBA) of a particular biofilm. So far, the lectin approach represents the only tool for in situ characterization of the glycoconjugate makeup in biofilm systems. Furthermore, lectin staining lends itself to other fluorescence techniques in order to correlate it with cellular biofilm constituents in general and glycoconjugate producers in particular. Full article

This review paper reports the applications of magnetic microbeads in biosensors and bio-bar code assays. Affinity biosensors are presented through different types of transducing systems: electrochemical, piezo electric or magnetic ones, applied to immunodetection and genodetection. Enzymatic biosensors are based on biofunctionalization through magnetic microbeads of a transducer, more often amperometric, potentiometric or conductimetric. The bio-bar code assays relie on a sandwich structure based on specific biological interaction of a magnetic microbead and a nanoparticle with a defined biological molecule. The magnetic particle allows the separation of the reacted target molecules from unreacted ones. The nanoparticles aim at the amplification and the detection of the target molecule. The bio-bar code assays allow the detection at very low concentration of biological molecules, similar to PCR sensitivity. Full article