This study isolated, determined, and quantified plant growth inhibitors in Japanese chestnut (
Castanea crenata Sieb. et Zucc), a deciduous species native to Japan and Korea. In laboratory assays,
C. crenata leaves showed strong inhibition on germination and seedling growth of
Echinochloa crus-galli
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This study isolated, determined, and quantified plant growth inhibitors in Japanese chestnut (
Castanea crenata Sieb. et Zucc), a deciduous species native to Japan and Korea. In laboratory assays,
C. crenata leaves showed strong inhibition on germination and seedling growth of
Echinochloa crus-galli (barnyardgrass),
Lactuca sativa (lettuce), and
Raphanus sativus (radish). Laboratory and greenhouse trials showed that leaves of
C. crenata appeared as a promising material to manage weeds, especially the dicot weeds. By GC-MS and HPLC analyses, gallic, protocatechuic,
p-hydroxybenzoic, caffeic, ferulic, ellagic, and cinnamic acids were identified and quantified, of which ellagic acid was present in the highest quantity (2.36 mg/g dried leaves). By column chromatography and spectral data (
1H- and
13C-NMR, IR, and LC-MS) analysis, a compound identified as 2α,3β,7β,23-tetrahydroxyurs-12-ene-28-oic acid (
1) was purified from the methanolic leaf extract of
C. crenata (0.93 mg/g dried leaves). This constituent showed potent inhibition on growth of
E. crus-galli, a problematic weed in agricultural practice. The inhibition of the compound
1 (IC
50 = 2.62 and 0.41 mM) was >5 fold greater than that of
p-hydroxybenzoic acid (IC
50 = 15.33 and 2.11 mM) on shoot and root growth of
E. crus-galli, respectively. Results suggest that the isolated the compound
1 has potential to develop natural herbicides to manage
E. crus-galli. This study is the first to isolate and identify 2α,3β,7β,23-tetrahydroxyurs-12-ene-28-oic acid in a plant and report its plant growth inhibitory potential.
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