Search Results (9)

Using genetic selection, Guide Dogs for the Blind (GDB) has reduced the incidence rate of canine hip dysplasia (CHD). However, given that CHD is polygenic and multifactorial, environmental factors may impact the expression of this developmental condition. The objective of this original, correlational research was to investigate the relationship between the substrate in the whelping pool used from birth to 3 weeks of age and CHD diagnosis. This retrospective study included 5649 dogs (Labrador Retrievers, Golden Retrievers, and Labrador–Golden Retriever crosses) at GDB. A total of 2785 dogs were whelped between 1 July 2012 and 28 November 2015, when the whelping pools were lined with newspaper, and 2864 dogs were whelped between 5 March 2016 and 29 December 2019, when the whelping pools were lined with fleece. Bayesian regression modeling of the CHD diagnoses and PennHIP distraction index scores identified that dogs in the fleece group had a lower risk for CHD diagnosis but similar PennHIP scores compared to the newspaper group. Golden Retrievers had the highest risk of CHD diagnosis and PennHIP scores, followed by Labrador–Golden Retriever crosses and finally Labrador Retrievers, with the lowest risk of CHD diagnosis and PennHIP scores. No sex differences were identified for CHD diagnosis, but males had lower PennHIP scores than females. These results provide correlational evidence that housing-related environmental factors during the first three weeks of development—specifically, the substrate in the whelping pool—are associated with lifelong orthopedic health. Full article

This work provides historical information and examines changes in the methods and equipment used in gold recovery and processing operations. Alluvial gold recovery methods, mainly based on gravity separation combined with mercury (amalgamation), have been applied since the early days of mining. Historically, mining gold from the riverbeds was first implemented in Ancient Anatolia (also called “Asia Minor”) and Ancient Greece. As a first attempt to recover gold, the traditional immersion of sheepskin in river water to trap alluvial gold was developed. This technique has been considered a milestone in the famous myth of the Golden Fleece. Since then, gold extraction and processing technologies have evolved. In this respect, Emperor Augustus developed hydraulic gold mining during the period of the Roman Empire. Subsequently, the innovative machines of Georgius Bauer (Agricola) were widely used during the Renaissance, while Spanish colonialists in America improved their techniques by observing the efficient methods of the natives. Finally, the “American Gold Rush” era was perhaps the most crucial period of the alluvial gold mining process. It took place along the rivers of America during the 19th and 20th centuries. Today, in the technologically advanced society, various gold mining machines, including spiral and jig concentrators, provide higher production rates and less environmental harm. Full article

This work examines alluvial gold deposit mining and processing methods from the Argonautic expedition until the Renaissance. According to Greek mythology, the mountain rivers of Colchis (Georgia) carried coarse sand and gold particles, which were washed in special wooden sluice boxes. The finer fractions were held in the lower part of the device, which was lined with sheepskin. Using a sheepskin to extract gold from riverbeds gave rise to the myth of the Golden Fleece. Afterwards, during the Roman Empire period, the mining of alluvial gold was performed manually until the discovery of hydraulic mining, a technique that contributed to a massive increase in production. At the same time, the Romans employed various techniques to separate the metals from the total mass of the ore. Gold refining was carried out through cupellation and the mercury amalgamation process. During the Renaissance, Georgius Bauer Agricola wrote De Re Metallica, one of the essential machinery books in mining in the 16th century. He developed a new type of pump to remove water, the uncontrolled flow of which caused significant problems in the underground mining process. The bucket chain pump, the “pater noster” pump, and the piston pump are some of the most innovative devices he presented in his work. Also, Agricola extensively referenced the recovery techniques for gold and other precious metals during the Archaic period that helped preserve the myth of the Argonautic expedition and the Golden Fleece. Full article

This article provides a comprehensive and chronological account of the technological advancements in alluvial gold mining. Gold has been a highly prized commodity throughout history and has played a significant role in humanity’s economic and cultural progress. The primitive methods of extracting gold from riverbeds were carried out in Ancient Anatolia and Ancient Greece. The earliest references to the extraction of gold from riverbeds can be traced back to the Argonautic expedition and the myth of the “Golden Fleece”. In this myth, fine gold particles were believed to be trapped within the skin of the fleece, thus alluding to the early practices of gold recovery from rivers. Since then, gold extraction and processing technologies have evolved. During the Roman Empire period, Emperor Augustus made significant advancements in hydraulic gold mining. He developed the “ground sluicing” method, which used water to wash away the overlying soil and expose the bedrock where gold deposits were located. Subsequently, the revolutionary pumps described by Georgius Bauer (Agricola) gained significant popularity during the Renaissance. Additionally, Spanish colonialists in America enhanced their techniques by observing the efficient methods employed by the indigenous populations. Finally, the “gold rush” era was perhaps the most crucial period in alluvial gold mining and took place in the rivers of America during the 19th–20th centuries. Today, technologies have evolved significantly, and exploring the evolution of primitive methods to the present is considered essential. Full article

Salinity stress is severely affecting modern horticulture and puts food security under threat for current and future generations. The aim of the present study was to evaluate the effect of saline conditions (three salinity levels: 2.0, 5.0 and 10.0 dS m⁻¹) on the growth, physiological processes and quality of two wild edible species (Urospermum picroides and Reichardia picroides) grown under three different cropping systems (pots indoors (GP) and outdoors (FP); and floating hydroponics (FH)). Our results indicate that high salinity affected growth parameters in all the studied cropping systems in the case of U. picroides, whereas R. picroides was not affected only when grown in pots outdoors. Moreover, total soluble solids content and titratable acidity in both species were not affected by high salinity for any of the cropping systems, except for in the case of FP system. Similarly, carotenoids content decreased under high salinity when both species were grown in the FP system. A varied effect was recorded for total phenolic compounds content in response to salinity levels, although the FP system resulted in considerably higher phenolics accumulation in both species, while proline content increased when plants were subjected to high salinity, regardless of the cropping system. The antioxidant activity also varied among the studied treatments for both assays (TEAC and FRAP), although cultivation outdoors in pots resulted in considerably higher values compared to the other systems. Finally, nitrate content showed decreasing trends with increasing salinity in plants grown in the GP (both species) and FP system (only U. picroides), whereas no significant differences in physiological parameters in comparison to the control treatment were recorded, except for the stomatal conductance (FP and GP system) and transpiration rate (FP) of R. picroides plants. In conclusion, it seems that the tested plant species responded differently to the salinity treatments but they both displayed a lack of severe stress even at high salinity. Full article

Material research in tissue engineering forms a vital link between basic cell research and animal research. Periodontal ligament cells (PDLCs, P) from the tooth have an osteogenic effect, whereas endothelial progenitor cells (EPCs, E) assist in neovascularization. In the present study, the interaction of PDLCs and EPCs with Tantalum (Ta, I) discs, either alone (IP or IE group) or in 1:1 (IPE) ratio was explored. Additionally, surface analysis of Ta discs with respect to different types and cycles of sterilization and disinfection procedures was evaluated. It was observed that Ta discs could be used for a maximum of three times, after which the changes in properties of Ta discs were detrimental to cell growth, irrespective of the type of the protocol. Cell-Disc’s analysis revealed that cell proliferation in the IE group at day 6 and day 10 was significantly higher (p < 0.05) than other groups. A cell viability assay revealed increased live cells in the IPE group than in the IP or IE group. Similarly, adhesion and penetration of cells in the IPE group were not only higher, but also had an increased thickness of cellular extensions. RT-PCR analysis revealed that on day 8, both osteogenic (ALP, RUNX-2, and BSP) and angiogenic genes (VEGFR-2, CD31) increased significantly in the IPE group as compared to the IP or IE groups (p < 0.05). In conclusion, Ta discs promoted cellular proliferation and increased osteogenic and angiogenic activity by augmenting RUNX-2 and VEGFR-2 activity. Full article

Smooth golden fleece (Urospermum dalechampii (L.) F.W. Schmidt) and prickly golden fleece (Urospermum picroides (L.) Scop. ex F.W. Schmid) are two wild edible plants used in traditional cuisine and folk medicine. In this research, the domestication of both species was tested for the first time using a floating system and two plant densities (412 and 824 plants m⁻²) to evaluate yield and quality. Some quality traits were also compared in cultivated plants and wild ones gathered in grasslands. The results show that both species are suitable for cultivation, although prickly golden fleece showed highest total phenols (132 mg 100 g⁻¹ fresh weight—f.w.) and total antioxidant activity (0.19 mg 100 g⁻¹ f.w.). At low sowing density, smooth golden fleece showed a nitrate content of about 7200 mg kg⁻¹ f.w., 38% higher than plants of the same species grown at high density and plants of prickly golden fleece. These results suggest that high density can be used to optimize yield in two harvests. By permitting modulation of nutrients and a product without soil residues, the floating system used in this study proved suitable for growing U. dalechhampii and U. picroides as new vegetables for the ready-to-eat production chain. Full article

In the last decade, vitamin D was in the spotlight in many fields of research. Despite numerous publications, its influence on reproductive health remains ambiguous. This paper presents an up-to-date review of current knowledge concerning the role of cholecalciferol in human reproduction. It covers various infertility issues, such as polycystic ovary syndrome, endometriosis, myoma-induced infertility, male infertility, premature ovary failure and in vitro fertilization techniques. Vitamin D deficiency, defined as serum concentration of 25-hydroxycalciferol of less than 50 nmol/L, is commonly noted more frequently than only in fertility clinic patients. It is a global trend that is observed in all age groups. The results of original publications dated up to 2015 have been summarized and discussed in a critical manner. Most experts agree that vitamin D supplementation is a necessity, particularly in women suffering from obesity, insulin resistance or small ovarian reserve, as well as in men with oligo- and asthenozoospermia if serum concentration should fall below 50 nmol/L (normal range up to 125 nmol/L). High concentration of vitamin D and its metabolites in decidua during the 1st trimester suggests its important role in the implantation process and a local immunological embryo-protection. On the other hand, evidence-based research did not prove a significant difference so far in ovulation stimulation or embryo development depending on vitamin D level. In one of the publications, it was also found that vitamin D binding protein (VDBP) has a molecular similarity to anti-sperm antibodies, and another one concluded that both low (<50 nmol/L) and high (>125 nmol/L) concentration of vitamin D are associated with decreased number and quality of spermatozoa in semen. Vitamin D is definitely not a Trojan Horse in reproductive health, since there were no adverse effects reported for vitamin D intake of up to 10,000 IU/day, but to proclaim it the Golden Fleece, more evidence is needed. Full article