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Keywords = HA-Orn
Authors = manfredini
Journal = Pharmaceutics

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17 pages, 3794 KiB  
Article
Design, Synthesis, Characterization, and In Vitro Evaluation of a New Cross-Linked Hyaluronic Acid for Pharmaceutical and Cosmetic Applications
by Sabrina Sciabica, Giovanni Tafuro, Alessandra Semenzato, Daniela Traini, Dina M. Silva, Larissa Gomes Dos Reis, Luisa Canilli, Massimo Terno, Elisa Durini, Silvia Vertuani, Anna Baldisserotto and Stefano Manfredini
Pharmaceutics 2021, 13(10), 1672; https://doi.org/10.3390/pharmaceutics13101672 - 13 Oct 2021
Cited by 18 | Viewed by 4632
Abstract
Hyaluronic acid (HA), an excellent biomaterial with unique bio properties, is currently one of the most interesting polymers for many biomedical and cosmetic applications. However, several of its potential benefits are limited as it is rapidly degraded by hyaluronidase enzymes. To improve the [...] Read more.
Hyaluronic acid (HA), an excellent biomaterial with unique bio properties, is currently one of the most interesting polymers for many biomedical and cosmetic applications. However, several of its potential benefits are limited as it is rapidly degraded by hyaluronidase enzymes. To improve the half-life and consequently increase performance, native HA has been modified through cross-linking reactions with a natural and biocompatible amino acid, Ornithine, to overcome the potential toxicity commonly associated with traditional linkers. 2-chloro-dimethoxy-1,3,5-triazine/4-methylmorpholine (CDMT/NMM) was used as an activating agent. The new product (HA–Orn) was extensively characterized to confirm the chemical modification, and rheological analysis showed a gel-like profile. In vitro degradation experiments showed an improved resistance profile against enzymatic digestions. Furthermore, in vitro cytotoxicity studies were performed on lung cell lines (Calu-3 and H441), which showed no cytotoxicity. Full article
(This article belongs to the Special Issue Application of Chitosan and Hyaluronan in Medicine)
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