Search Results (3)

Cimicifuga heracleifolia is a perennial herb that belongs to the Ranunculaceae family. Its dried rhizomes are a source of Cimicifugae Rhizoma, a traditional Chinese medicine used for detoxification, the treatment of febrile diseases, and the prevention of pathogenic invasion. In the present study, a phytochemical investigation of the rhizomes of C. heracleifolia resulted in the isolation of three indole alkaloids (1–3) and seven phenolic amides (4–10), including three new compounds, 6-methoxy-3-(3-methyl-1-oxo-2-butenyl) 1H indole (1), (3R)-1-(β-d-glucopyranosyl)-3-hydroxy-3-(3-methylbutyl)-2-oxindole (3), and N-acetyldopamine-3-O-β-d-allopyranoside (4). Their structures were elucidated using extensive physicochemical and spectroscopic analyses. All the isolated compounds were evaluated for their inhibitory activity against soluble epoxide hydrolase (sEH). The results showed that cimicifugamide A (6) exhibited the most potent inhibitory activity, with an IC₅₀ value of 8.74 μM, followed by cimicifugamide (7), demethoxycimicifugamide (8), and N-trans-feruloyl tyramine (10), with IC₅₀ values ranging from 15.63 to 20.58 μM. Kinetic analysis revealed that compound 6 inhibited sEH through a non-competitive mechanism. Full article

Coinfection caused by various genotypes of porcine epidemic diarrhea virus (PEDV) is a new disease situation. We previously reported the coexistence of PEDV strains containing different ORF3 genotypes in China. In this study, the PEDV strains 17GXCZ-1ORF3d and 17GXCZ-1ORF3c were isolated and plaque-purified from the same piglet, which had a natural large deletion at the 172–554 bp position of the ORF3 gene or possessed a complete ORF3 gene, respectively. Meanwhile, 17GXCZ-1ORF3d had >99% nt identity with 17GXCZ-1ORF3c in the 5′UTR, ORF1a/1b, S, E, M, N and 3′UTR regions but only demonstrated low nucleotide identities (80.5%) in the ORF3 gene. To elucidate the pathogenicity, 7-day-old piglets were infected. Piglets infected with these two PEDV strains exhibited severe clinical signs and shed the virus at the highest level within 96 hpi. Compared with the piglets inoculated with the 17GXCZ-1ORF3c strain, the piglets inoculated with the 17GXCZ-1ORF3d strain had higher mortality rates (75% vs. 50%), an earlier onset of clinical signs with a significantly higher diarrhea score, lower VH:CD ratios and a higher percentage of PEDV-positive enterocytes. This study is the first to report PEDV coinfections with different ORF3 genotypes, and a PEDV strain with a large deletion in the ORF3 gene might have the advantage of a potential genetic marker, which would be useful during vaccine development. Full article

Conventional methods for Aerosol Optical Depth (AOD) retrieval are limited to areas with low reflectance such as water or vegetated areas because the satellite signals from the aerosols in these areas are more obvious than those in areas with higher reflectance such as urban and sandy areas. Land Surface Reflectance (LSR) is the key parameter that must be estimated accurately. Most current methods used to estimate AOD are applicable only in areas with low reflectance. It has historically been difficult to estimate the LSR for bright surfaces because of their complex structure and high reflectance. This paper provides a method for estimating LSR for AOD retrieval in bright areas, and the method is applied to AOD retrieval for Landsat 8 Operational Land Imager (OLI) images at 500 m spatial resolution. A LSR database was constructed with the MODerate-resolution Imaging Spectroradiometer (MODIS) surface reflectance product (MOD09A1), and this database was also used to estimate the LSR of Landsat 8 OLI images. The AOD retrieved from the Landsat 8 OLI images was validated using the AOD measurements from four AErosol RObotic NETwork (AERONET) stations located in areas with bright surfaces. The MODIS AOD product (MOD04) was also compared with the retrieved AOD. The results demonstrate that the AOD retrieved with the new algorithm is highly consistent with the AOD derived from ground measurements, and its precision is better than that of MOD04 AOD products over bright areas. Full article