Search Results (9)

Prostate cancer (PCa) is a high-prevalence disease usually characterized by metastatic spread to the pelvic lymph nodes and bones and the development of visceral metastases only in the late stages of disease. Positron Emission Tomography (PET) plays a key role in the detection of PCa metastases. Several PET radiotracers are used in PCa patients according to the stage and pathological features of the disease, in particular ⁶⁸Ga/¹⁸F-prostate-specific membrane antigen (PSMA) ligands. Moreover, 2-deoxy-2-[18F]fluoro-D-glucose ¹⁸F-FDG PET usually shows metastases in the late stages of disease, when dedifferentiated neoplastic clones lose PSMA expression. In some cases, PCa patients may present atypical sites of metastases, with uncommon appearance at PET imaging with different radiotracers. We present the case of a patient with biochemical recurrence of PCa (ISUP Grade Group IV; PSA 4.7 ng/mL) showing atypical sites of metastases (the testis and multiple lung nodules) with absent PSMA expression and high [¹⁸F]FDG avidity. The patient showed diffuse positivity to alpha-methylacyl-coenzyme A racemase (AMACR). Moreover, a literature review was performed by collecting cases of PCa patients with atypical metastatic spread detected via PET imaging, with the aim of highlighting the relationship between atypical sites of metastases, imaging presentation, and pathology findings. Full article

Personal Emergency Response Systems (PERSs) are fall-detection devices supporting users in any situation. No previous studies have investigated the differences in events and the use of PERS between users financially supported by public authorities (public users) and those who privately afford the PERS cost (private users). More than two years of data collected by the Telemergency Operation Centre (TOC) were downloaded. All users who sent at least one real alert to request support were included. No differences were found for falls (37, 16.7% vs. 95, 13.4%) and medical problems (46, 20.7% vs. 122, 17.2%). The dispatch of an ambulance was necessary for all medical problems, while for falls, this was only in half of cases. Public users significantly asked more for service demand, while private users asked for support calls. The TOC staff directly managed most of the service demands (398, 97.3%) and support calls. PERS could be a valid instrument for promoting independent living and helping manage chronic conditions in older adults. The results suggest that PERSs might improve in-home care services, facilitating the connection to in-home services. Full article

Dry-cured ham (DCH) could support the growth of Staphylococcus aureus as a halotolerant bacterium, which may compromise the shelf-stability of the product according to the growth/no growth boundary models and the physicochemical parameters of commercial DCH. In the present study, the behavior of S. aureus is evaluated in sliced DCH with different water activity (a_w 0.861–0.925), packaged under air, vacuum, or modified atmosphere (MAP), and stored at different temperatures (2–25 °C) for up to 1 year. The Logistic and the Weibull models were fitted to data to estimate the primary kinetic parameters for the pathogen Log₁₀ increase and Log₁₀ reduction, respectively. Then, polynomial models were developed as secondary models following their integration into the primary Weibull model to obtain a global model for each packaging. Growth was observed for samples with the highest a_w stored at 20 and 25 °C in air-packaged DCH. For lower a_w, progressive inactivation of S. aureus was observed, being faster at the lowest temperature (15 °C) for air-packaged DCH. In contrast, for vacuum and MAP-packaged DCH, a higher storage temperature resulted in faster inactivation without a significant effect of the product a_w. The results of this study clearly indicate that the behavior of S. aureus is highly dependent on factors such as storage temperature, packaging conditions and product a_w. The developed models provide a management tool for evaluating the risk associated with DCH and for preventing the development of S. aureus by selecting the most appropriate packaging according to a_w range and storage temperature. Full article

High pressure processing (HPP) inactivates pathogens and increases the safety of ready-to-eat meat products. The high-pressure lethality and the behavior of the surviving cells after HPP depends on process parameters (pressure and time), microorganism and matrix characteristics. The aim of the present study was to quantify the impact of pressure level, water activity (a_w), and fat content on the behavior of Salmonella spp. and Listeria monocytogenes during refrigerated storage of dry-cured ham after high-pressure processing. Salmonella enterica serotype London CTC1003 and L. monocytogenes CTC1034 were inoculated at ca. 7 log cfu/g in dry-cured ham of different a_w (0.87–0.98), vacuum packaged, pressurized from 300 to 852 MPa for 5 min, and stored at 7 °C for up to 2 months. Salmonella and L. monocytogenes populations were monitored by plate count during the storage of the hams. The gamma concept was used to quantify the individual effects of a_w and storage temperature on the pathogen growth/no-growth behavior in pressurized dry-cured ham. The Weibull (inactivation) or Logistic (growth) primary models were fitted to the log change of pathogen levels during storage of dry-cured ham after pressurization. According to the gamma approach, the refrigeration temperature and a_w were the main factors limiting the growth of Salmonella and L. monocytogenes, respectively, in dry-cured ham. Under conditions not allowing growth, the effect of increasing pressures on the microbial inactivation depended on the a_w of dry-cured ham and the pathogen; dry-cured ham with high fat content with an a_w ≥ 0.95 enhanced the inactivation of Salmonella whereas it reduced that of L. monocytogenes. Under conditions allowing growth of L. monocytogenes, the increase in a_w from 0.96 to 0.98 reduced the lag time with no apparent impact on the growth rate. Full article

Formulations with lactate as an antimicrobial and high-pressure processing (HPP) as a lethal treatment are combined strategies used to control L. monocytogenes in cooked meat products. Previous studies have shown that when HPP is applied in products with lactate, the inactivation of L. monocytogenes is lower than that without lactate. The purpose of the present work was to identify the molecular mechanisms underlying the piezo-protection effect of lactate. Two L. monocytogenes strains (CTC1034 and EGDe) were independently inoculated in a cooked ham model medium without and with 2.8% potassium lactate. Samples were pressurized at 400 MPa for 10 min at 10 °C. Samples were subjected to RNA extraction, and a shotgun transcriptome sequencing was performed. The short exposure of L. monocytogenes cells to lactate through its inoculation in a cooked ham model with lactate 1h before HPP promoted a shift in the pathogen’s central metabolism, favoring the metabolism of propanediol and ethanolamine together with the synthesis of the B12 cofactor. Moreover, the results suggest an activated methyl cycle that would promote modifications in membrane properties resulting in an enhanced resistance of the pathogen to HPP. This study provides insights on the mechanisms developed by L. monocytogenes in response to lactate and/or HPP and sheds light on the understanding of the piezo-protective effect of lactate. Full article

Cytokine-induced killer (CIK) cells are advanced therapy medicinal products, so their production and freezing process has to be validated before their clinical use, to verify their stability as a drug formulation according to the good manufacturing practice (GMP) guidelines. We designed a stability program for our GMP-manufactured CIK cells, evaluating the viability, identity and potency of cryopreserved CIK cells at varying time periods from freezing, and compared them with fresh CIK cells. We evaluated the effects of the cryopreservation method, transportation, and the length of time of different process phases (pre-freezing, freezing and post-thawing) on the stability of CIK cells. This included a worst case for each stage. The expanded CIK cells were viable for up to 30 min from the addition of the freezing solution, when transported on dry ice within 48 h once frozen, within 60 min from thawing and from 12 months of freezing while preserving their cytotoxic effects. The reference samples, cryopreserved simultaneously in tubes and following the same method, were considered representative of the batch and useful in the case of further analysis. Data obtained from this drug stability program can inform the accurate use of CIK cells in clinical settings. Full article

Phasor measurement units (PMUs), which are the key components of a synchrophasor-based wide area monitoring system (WAMS), were historically conceived for transmission networks. The current trend to extend the benefits of the synchrophasor technology to distribution networks requires the PMU to also provide trustworthy information in the presence of signals that can occur in a typical distribution grid, including the presence of severe power quality (PQ) issues. In this framework, this paper experimentally investigates the performance of PMUs in the presence of one of the most important PQ phenomena, namely the presence of voltage fluctuations that generate the disturbance commonly known as flicker. The experimental tests are based on an ad-hoc high-accuracy measurement setup, where the devices under test are considered as “black boxes” to be characterized in the presence of the relevant signals. Two simple indices are introduced for the comparison among the different tested PMUs. The results of the investigation highlight possible critical situations in the interpretation of the measured values and provide a support for both the design of a new generation of PMUs and the possible development of an updated synchrophasor standard targeted to distribution systems. Full article

The unprecedented growth in foreign direct investment in the last few decades has caused drastic changes in the labor markets of the host countries. The major part of FDI takes place in low-tech industries, where the wages and skills are low, or in high-tech, where they offer a wage premium for the highly skilled workers. This mechanism may increase the polarization of employment into high-wage and low-wage jobs, at the expense of middle-skill jobs. This paper looks at the effects of two types of FDI inflows, namely foreign investment in high-skill and low-skill activities, on job polarization. We match data on greenfield FDI aggregated by country and sector with data on employment by occupational skill to investigate the extent to which different types of greenfield FDI are responsible for skill polarization. Our results show that low-skill foreign investment shifts employment from high- to medium- and low-skill jobs, while skill-intensive FDI generally leads to skill upgrading. Only FDI in information and communication technology (ICT) is associated with job polarization, but only when accounting for the plurality of job polarization patterns across European sectors. Full article

For the last decades, nanocomposites materials have been widely studied in the scientific literature as they provide substantial properties enhancements, even at low nanoparticles content. Their performance depends on a number of parameters but the nanoparticles dispersion and distribution state remains the key challenge in order to obtain the full nanocomposites’ potential in terms of, e.g., flame retardance, mechanical, barrier and thermal properties, etc., that would allow extending their use in the industry. While the amount of existing research and indeed review papers regarding the formulation of nanocomposites is already significant, after listing the most common applications, this review focuses more in-depth on the properties and materials of relevance in three target sectors: packaging, solar energy and automotive. In terms of advances in the processing of nanocomposites, this review discusses various enhancement technologies such as the use of ultrasounds for in-process nanoparticles dispersion. In the case of nanocoatings, it describes the different conventionally used processes as well as nanoparticles deposition by electro-hydrodynamic processing. All in all, this review gives the basics both in terms of composition and of processing aspects to reach optimal properties for using nanocomposites in the selected applications. As an outlook, up-to-date nanosafety issues are discussed. Full article