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Organochlorine pesticides are groups of chemicals applied to prevent pest and insect infestation. This study was aimed at investigating the concentration, potential sources, cancer risk and ecological toxicity of organochlorine pesticides (OCPs) in Huangpi district, Wuhan, China. Eight OCPs in soil samples collected from four land-use types at depths of 0–10 and 10–20 cm were examined. Sample extraction was carried out by solid phase matrix extraction method and analyzed using Agilent gas chromatograph 7890B equipped with electron capture detectors (ECD). The total concentration of OCPs ranged from 0.00–32.7 ng g⁻¹ in the surface and 0.01–100.45 ng g⁻¹ in the subsurface soil layer. Beta hexachlorocyclohexanes (β-HCH) with 2.20 and 7.71 ng g⁻¹ in the surface and subsurface soil layers, respectively, was the dominant compound. The mean concentrations of OCPs in all samples were less than the threshold values for hexachlorocyclohexanes (HCHs) and dichlorodiphenyltrichloroethane (DDTs) in China soil. Concentration of OCPs in the four land-use types were in the order of: paddy field > barren land > farmland > plastic greenhouse. Results of composition analysis revealed recent application of lindane as a major and historical use of new technical HCHs as a minor source of HCHs. On the other hand, application of new technical p,p’-DDT is the main source of DDTs in the study area. The estimated lifetime average daily dose, incremental lifetime cancer risks and hazard quotient values revealed that there is less likelihood of carcinogenic and noncarcinogenic health risks on the local residents. Full article