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Authors = Marie Czinnerová

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17 pages, 3099 KiB  
Article
Thermally Enhanced Biodegradation of TCE in Groundwater
by Petra Najmanová, Jana Steinová, Marie Czinnerová, Jan Němeček, Roman Špánek, Vladislav Knytl and Martin Halecký
Water 2022, 14(21), 3456; https://doi.org/10.3390/w14213456 - 29 Oct 2022
Cited by 3 | Viewed by 2914
Abstract
In situ remediation is usually restricted by temperature, lack of substrate for reductive dechlorination (anaerobic respiration), the presence of dehalogenating microorganisms, and specific bedrock conditions. In this work, trichloroethene (TCE) degradation was studied by a number of methods, from physical–chemical analyses to molecular [...] Read more.
In situ remediation is usually restricted by temperature, lack of substrate for reductive dechlorination (anaerobic respiration), the presence of dehalogenating microorganisms, and specific bedrock conditions. In this work, trichloroethene (TCE) degradation was studied by a number of methods, from physical–chemical analyses to molecular biological tools. The abundance changes in dechlorinating bacteria were monitored using real-time PCR. The functional genes vcrA and bvcA as well as the 16S rRNA specific for representatives of genera Dehalococcoides, Dehalobacter, and Desulfitobacterium were monitored. Furthermore, the sulfate-reducing bacteria and denitrifying bacteria were observed by amplifying the functional genes apsA and nirK. The elevated temperature and the substrate (whey) addition significantly affected TCE dechlorination. The chlorine index decreased after nine weeks from 2.5 to 0.1 at 22 °C, to 1.1 at 17 °C and 1.7 at 12 °C and complete dechlorination was achieved at 22 °C with whey addition. The achieved results of this work show the feasibility and effectiveness of biological dechlorination of TCE enhanced with elevated temperature and whey addition. Full article
(This article belongs to the Section Water Quality and Contamination)
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12 pages, 486 KiB  
Article
Biodegradation of High Concentrations of Aliphatic Hydrocarbons in Soil from a Petroleum Refinery: Implications for Applicability of New Actinobacterial Strains
by Josef Trögl, Catherine Oluwakemi Esuola, Sylvie Kříženecká, Pavel Kuráň, Lenka Seidlová, Petra Veronesi-Dáňová, Jan Popelka, Olubukola Oluranti Babalola, Pavel Hrabák, Marie Czinnerová, Eva Kakosová, Alena Ševců and Dirk Tischler
Appl. Sci. 2018, 8(10), 1855; https://doi.org/10.3390/app8101855 - 9 Oct 2018
Cited by 19 | Viewed by 4773
Abstract
At present, there is great demand for new resistant and metabolically active strains of biodegrading bacteria capable of degrading high concentrations of petroleum pollutants. In this study, we undertook a series of pot-based biodegradation experiments on soil from a petroleum refinery lagoon heavily [...] Read more.
At present, there is great demand for new resistant and metabolically active strains of biodegrading bacteria capable of degrading high concentrations of petroleum pollutants. In this study, we undertook a series of pot-based biodegradation experiments on soil from a petroleum refinery lagoon heavily polluted with aliphatic hydrocarbons (81.6 ± 2.5 g·kg−1 dry weight) and metals. Periodical bioaugmentation with either a mixture of isolated degraders identified as Bacillus sp. and Ochrobactrum sp. or biostimulation with nutrient medium, singly or in combination, did not produce any significant decrease in hydrocarbons, even after 455 days. Inoculation with Gordonia rubripertincta CWB2 and Rhodococcus erythropolis S43 in iron-limited media, however, resulted in a significant decrease in hydrocarbons 45 days after bioaugmentation. These actinobacterial strains, therefore, show significant potential for bioremediation of such highly polluted soils. Full article
(This article belongs to the Section Applied Biosciences and Bioengineering)
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