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Authors = Lídia M. Dias

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12 pages, 1107 KiB  
Article
Analysis of Epstein–Barr Virus (EBV) and PD-L1 Expression in Nasopharyngeal Carcinoma Patients in a Non-Endemic Region
by Josiane M. Dias, Iara V. V. Santana, Vinicius D. da Silva, André L. Carvalho and Lidia M. R. B. Arantes
Int. J. Mol. Sci. 2022, 23(19), 11720; https://doi.org/10.3390/ijms231911720 - 3 Oct 2022
Cited by 8 | Viewed by 2624
Abstract
Background: The purpose of this study was to evaluate the status of Epstein–Barr virus (EBV) infection and the expression of programmed cell death ligand-1 (PD-L1) in tumor samples from patients with nasopharyngeal carcinoma (NPC). Methods: Evaluation of EBV infection was performed through the [...] Read more.
Background: The purpose of this study was to evaluate the status of Epstein–Barr virus (EBV) infection and the expression of programmed cell death ligand-1 (PD-L1) in tumor samples from patients with nasopharyngeal carcinoma (NPC). Methods: Evaluation of EBV infection was performed through the detection of EBV-encoded small ribonucleic acids (EBER) by in situ hybridization, and PD-L1 expression was performed through immunohistochemistry. Results: In total, 124 samples were evaluated for EBER and 120 for PD-L1 expression. A total of 86.3% of cases were positive for EBER and 55.8% were positive for PD-L1. There was a correlation between EBER positivity and the presence of undifferentiated carcinoma histology (p = 0.007) as well as the absence of tobacco history (p = 0.019). There was a correlation between PD-L1 expression and EBER positivity (p = 0.004). There was no statistically significant difference between overall survival (OS) and EBER (p = 0.290) or PD-L1 (p = 0.801) expression. Conclusions: This study corresponds to one of the largest cohorts of NPC in a non-endemic region. Phase III studies with checkpoint inhibitors are ongoing and may provide more data about the role of PD-L1 expression in this disease. Full article
(This article belongs to the Special Issue State-of-the-Art Molecular Oncology in Brazil)
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12 pages, 2309 KiB  
Article
Xylanase Production by Talaromyces amestolkiae Valuing Agroindustrial Byproducts
by Giórgia S. Barbieri, Heitor B. S. Bento, Fernanda de Oliveira, Flávio P. Picheli, Lídia M. Dias, Fernando Masarin and Valéria C. Santos-Ebinuma
BioTech 2022, 11(2), 15; https://doi.org/10.3390/biotech11020015 - 17 May 2022
Cited by 15 | Viewed by 4299
Abstract
In general, agroindustrial byproducts can be easily assimilated by several microorganisms due to their composition, which is rich in carbohydrates. Therefore, they could be appropriate for use as raw materials in a sustainable refinery concept, including the production of hydrolytic enzymes with industrial [...] Read more.
In general, agroindustrial byproducts can be easily assimilated by several microorganisms due to their composition, which is rich in carbohydrates. Therefore, they could be appropriate for use as raw materials in a sustainable refinery concept, including the production of hydrolytic enzymes with industrial applicability. In this work, xylanase production by the filamentous fungi Talaromyces amestolkiae in submerged culture was evaluated using five agroindustrial byproducts, namely, wheat bran, citrus pulp, rice bran, peanut skin, and peanut shell. Firstly, the aforementioned byproducts were characterized in terms of cellulose, xylan, lignin, and extractives. Next, production studies were performed, and wheat bran generated the highest enzymatic activity (5.4 U·mL−1), probably because of its large amount of xylan. Subsequently, a factorial design was performed to evaluate the independent variables yeast extract, wheat bran, K2HPO4, and pH, aiming to improve the variable response, xylanase activity. The condition that promoted the highest production, 13.02 U·mL−1 (141% higher than the initial condition), was 20 g·L−1 wheat bran, 2.5 g·L−1 yeast extract, 3 g·L−1 K2HPO4, and pH 7. Thus, industrial byproducts with a high content of xylan can be used as a culture medium to produce xylanase enzymes with a Talaromyces strain through an economical and sustainable approach. Full article
(This article belongs to the Section Industry, Agriculture and Food Biotechnology)
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