Search Results (4)

Multiple drug resistance (MDR) has gained pronounced attention among Enterobacterales. The transfer of multiple antimicrobial resistance genes, frequently carried on conjugative incompatibility F (IncF) plasmids and facilitating interspecies resistance transmission, has been linked to Salmonella spp. and E. coli in broilers. In Egypt, the growing resistance is exacerbated by the limited clinical efficacy of many antimicrobials. In this study, IncF groups were screened and characterized in drug-resistant Salmonella spp. and E. coli isolated from broilers. The antimicrobial resistance profile, PCR-based replicon typing of bacterial isolates pre- and post-plasmid curing, and IncF replicon allele sequence typing were investigated. Five isolates of E. coli (5/31; 16.13%) and Salmonella spp. (5/36; 13.89%) were pan-susceptible to the examined antimicrobial agents, and 85.07% of tested isolates were MDR and extensively drug-resistant (XDR). Twelve MDR and XDR E. coli and Salmonella spp. isolates were examined for the existence of IncF replicons (FII, FIA, and FIB). They shared resistance to ampicillin, ampicillin/sulbactam, amoxicillin/clavulanate, doxycycline, cefotaxime, and colistin. All isolates carried from one to two IncF replicons. The FII-FIA-FIB+ and FII-FIA+FIB- were the predominant replicon patterns. FIB was the most frequently detected replicon after plasmid curing. Three XDR E. coli isolates that were resistant to 12–14 antimicrobials carried a newly FIB replicon allele with four nucleotide substitutions: C99→A, G112→T, C113→T, and G114→A. These findings suggest that broilers are a significant reservoir of IncF replicons with highly divergent IncF-FIB plasmid incompatibility groups circulating among XDR Enterobacterales. Supporting these data with additional comprehensive epidemiological studies involving replicons other than the IncF can provide insights for implementing efficient policies to prevent the spreading of new replicons to humans. Full article

A causal vector autoregressive (CVAR) model is introduced for weakly stationary multivariate processes, combining a recursive directed graphical model for the contemporaneous components and a vector autoregressive model longitudinally. Block Cholesky decomposition with varying block sizes is used to solve the model equations and estimate the path coefficients along a directed acyclic graph (DAG). If the DAG is decomposable, i.e., the zeros form a reducible zero pattern (RZP) in its adjacency matrix, then covariance selection is applied that assigns zeros to the corresponding path coefficients. Real-life applications are also considered, where for the optimal order $p\ge 1$ of the fitted CVAR$\left(p\right)$ model, order selection is performed with various information criteria. Full article

Evaluating a novel fungal strain’s pathogenicity to important pests and their involved immune responses may give crucial data on a broad scale for future use in pest management strategies. Date palms are mostly destroyed by invading populations of red palm weevils; thus, developing natural biopesticides for them requires a comprehensive screening program of plant secondary metabolites. In this research, we examined the pathogenicity of a new strain of Beauveria bassiana on an important agricultural pest, Rhynchophorus ferrugineus, by measuring the relative activity of defensive enzymes and detoxifying enzymes in certain larval instars. Our findings reveal that the B. bassiana strain may infect the instars of R. ferrugineus, and its pathogenicity to the larvae steadily increases as the spore concentration increases. Seven days after inoculation, the LC₅₀ (the median lethal concentration) of B. bassiana was 490.42 × 10⁵ and 2974.47 × 10⁸ spores/mL for the second and fourth instar R. ferrugineus, respectively, and the LC₅₀ of B. bassiana for each R. ferrugineus instar decreased with infection time, indicating a significant dose effect. Infected R. ferrugineus larvae of the second instars showed considerable changes in the activity of both protecting and detoxifying enzymes (peroxidase, catalase, superoxide dismutase, Cytochrome P450, glutathione S transferase (GST), and esterase) as infection time progressed. In addition, R. ferrugineus larvae that were infected with B. bassiana had enzyme activity that persisted from 24 to 48 h, which was much longer than in the control group. Lethality of B. bassiana resulted in elevated expressions of GST, Esterase, and Cytochrome P450 responsive genes. In conclusion, the results of this research indicate that B. bassiana may be utilized as a bio-insecticide to suppress young larvae of R. ferrugineus in an integrated pest management program. Full article

The congestion charging scheme is regarded as a successful measure to reduce traffic-related problems. However, low general acceptability of the public for implementing such a scheme is a barrier against its success. In this research, an online pre-designed survey was conducted in five capitals (Budapest, Tunis, Amman, Ulaanbaatar, and Damascus) to define the factors that affect congestion charging acceptability the most. The results of relationships between the studied factors like travel behavior and acceptance of the congestion charging scheme show an irregular pattern in each city. It indicates that the identity of each city and its general policy implications determine which factors significantly affect the public acceptability of congestion charging scheme. In Amman and Budapest, most of the predictors have no statistical effect on the schemes’ public acceptability. Consistent with previous researches, on the other hand, the results demonstrate that the schemes’ effectiveness is crucial and affects the acceptability significantly in all cities. At the same time, it shows that the “prior scheme knowledge” factor has a significant direct effect on the acceptability level in three cities (Damascus, Tunis, and Ulaanbaatar). Full article