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African swine fever (ASF) is one of the most important and serious contagious hemorrhagic viral diseases affecting domestic pigs and wild boar and is associated with high mortality rates while also having an extensive sanitary and socioeconomic impact on the international trade of animal and swine products. The early detection of the disease is often hampered by inadequate surveillance. Among the surveillance strategies used, passive surveillance of wild boars is considered the most effective method for controlling the African swine fever virus (ASFV). Otherwise, the design of a sufficiently sensitive ASF surveillance system requires a solid understanding of the epidemiology related to the local eco-social context, especially in the absence of virus detection. Even if the number of carcasses needed to demonstrate ASF eradication has been established, the scientific context lacks detail compared to protocols applied in the active search for wild boar carcasses. The aim of this study was to describe the protocol applied in the active search for carcasses, providing detailed information on the number of people and dogs as well as the amount of time and space used within the Mediterranean area. Using a specific tool developed to record, trace, and share field data (the GAIA observer app), a total of 33 active searches for wild boar carcasses were organized during 2021–2023. Most of these searches were planned to find carcasses that had previously been reported by hunters. A total of 24 carcasses were found, with only 2 carcasses not previously reported. The final protocol applied involved four people, with an average speed of 1.5 km/h. When a carcass had been previously reported, about 2 km of distance had to be covered in about 1.5 h to find the carcass, and even less time was spent when a dog (untrained) was present. In conclusion, it can be stated that, when searching for carcasses, solid collaboration with local hunters or other forest visitors is necessary to ensure carcasses are reported. The process involves small groups of experts actively searching for carcasses, possibly with the use of hunting dogs without special training. The data presented could be of valid support for those countries characterized by Mediterranean vegetation that are faced with the need to plan active carcass searches. Full article

Isoxazoline is a nitrogen- and oxygen-containing five-membered heterocyclic scaffold with diverse biological profiles such as antimicrobial, fungicidal, anticancer, antiviral, analgesic and anti-inflammatory activity. Accordingly, the use of this peculiar structural framework in drug discovery is a successful strategy for the development of new drug candidates. Here, a chiral saccharin/isoxazoline hybrid was considered to investigate the tendency of the imine moiety of the heterocyclic ring to tautomerize to the enamine form in the presence of a basic catalyst. The pseudo-first-order rate constants for the base-catalyzed tautomerization process were measured in different solvents and at different temperatures by off-column kinetic experiments based on the amylose (3,5-dimethylphenylcarbamate)-type chiral stationary phase. The kinetic results obtained in this study may be a useful aid in the perspective of designing experimental conditions to control the stereointegrity of these types of pharmacologically active compounds and drive their synthesis toward the preferred, imine or enamine, tautomer. Full article

The Mediterranean region is one of the most responsive areas to climate change and was identified as a major “hot-spot” based on global climate change analyses. This study provides insight into local climate changes in the Mediterranean region under the scope of the InTheMED project, which is part of the PRIMA programme. Precipitation and temperature were analyzed in an historical period and until the end of this century for five pilot sites, located between the two shores of the Mediterranean region. We used an ensemble of 17 Regional Climate Models, developed in the framework of the EURO-CORDEX initiative, under two Representative Concentration Pathways (RCP4.5 and RCP8.5). Over the historical period, the temperature presents upward trends, which are statistically significant for some sites, while precipitation does not show significant tendencies. These trends will be maintained in the future as predicted by the climate models projections: all models indicate a progressive and robust warming in all study areas and moderate change in total annual precipitation, but some seasonal variations are identified. Future changes in droughts events over the Mediterranean region were studied considering the maximum duration of the heat waves, their peak temperature, and the number of consecutive dry days. All pilot sites are expected to increase the maximum duration of heat waves and their peak temperature. Furthermore, the maximum number of consecutive dry days is expected to increase for most of the study areas. Full article