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13 pages, 2890 KiB

Open AccessArticle

Three-Dimensional Surface Reconstruction for Specular/Diffuse Composite Surfaces

by Chung-Hsuan Huang, Ssu-Chia He, Tsung-Yu Chen, Chau-Jern Cheng and Han-Yen Tu

Sensors 2024, 24(24), 7942; https://doi.org/10.3390/s24247942 - 12 Dec 2024

Cited by 2 | Viewed by 1115

This paper presents an effective three-dimensional (3D) surface reconstruction technique aimed at profiling composite surfaces with both specular and diffuse reflectance. Three-dimensional measurements based on fringe projection techniques perform well on diffuse reflective surfaces; however, when the measurement targets contain both specular and diffuse components, the efficiency of fringe projection decreases. To address this issue, the proposed technique integrates digital holography into the fringe projection setup, enabling the simultaneous capture of both specular and diffuse reflected light in the same optical path for full-field surface profilometry. Experimental results demonstrate that this technique effectively detects and accurately reconstructs the 3D profiles of specular and diffuse reflectance, with fringe analysis providing the absolute phase of composite surfaces. The experiments validate the effectiveness of this technique in the 3D surface measurement of integrated circuit carrier boards with chips exhibiting composite surfaces. Full article

(This article belongs to the Special Issue Imaging and Sensing in Optics and Photonics)

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12 pages, 2272 KiB

Open AccessArticle

Label-Free Three-Dimensional Morphological Characterization of Cell Death Using Holographic Tomography

by Chung-Hsuan Huang, Yun-Ju Lai, Li-Nian Chen, Yu-Hsuan Hung, Han-Yen Tu and Chau-Jern Cheng

Sensors 2024, 24(11), 3435; https://doi.org/10.3390/s24113435 - 26 May 2024

Cited by 3 | Viewed by 1833

Abstract

This study presents a novel label-free approach for characterizing cell death states, eliminating the need for complex molecular labeling that may yield artificial or ambiguous results due to technical limitations in microscope resolution. The proposed holographic tomography technique offers a label-free avenue for capturing precise three-dimensional (3D) refractive index morphologies of cells and directly analyzing cellular parameters like area, height, volume, and nucleus/cytoplasm ratio within the 3D cellular model. We showcase holographic tomography results illustrating various cell death types and elucidate distinctive refractive index correlations with specific cell morphologies complemented by biochemical assays to verify cell death states. These findings hold promise for advancing in situ single cell state identification and diagnosis applications. Full article

(This article belongs to the Special Issue Optical Instruments and Sensors and Their Applications)

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18 pages, 4909 KiB

Open AccessReview

Holographic Optical Tweezers: Techniques and Biomedical Applications

by Hui-Chi Chen and Chau-Jern Cheng

Appl. Sci. 2022, 12(20), 10244; https://doi.org/10.3390/app122010244 - 12 Oct 2022

Cited by 34 | Viewed by 5567

Abstract

Holographic optical tweezers (HOT) is a programmable technique used for manipulation of microsized samples. In combination with computer-generation holography (CGH), a spatial light modulator reshapes the light distribution within the focal area of the optical tweezers. HOT can be used to realize real-time multiple-point manipulation in fluid, and this is useful in biological research. In this article, we summarize the HOT technique, discuss its recent developments, and present an overview of its biological applications. Full article

(This article belongs to the Special Issue Photosensitive Materials and Their Applications)

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10 pages, 6957 KiB

Open AccessArticle

Investigation of Nonlinear Optical Properties of Quantum Dots Deposited onto a Sample Glass Using Time-Resolved Inline Digital Holography

by Andrey V. Belashov, Igor A. Shevkunov, Ekaterina P. Kolesova, Anna O. Orlova, Sergei E. Putilin, Andrei V. Veniaminov, Chau-Jern Cheng and Nikolay V. Petrov

J. Imaging 2022, 8(3), 74; https://doi.org/10.3390/jimaging8030074 - 16 Mar 2022

Cited by 3 | Viewed by 2817

Abstract

We report on the application of time-resolved inline digital holography in the study of the nonlinear optical properties of quantum dots deposited onto sample glass. The Fresnel diffraction patterns of the probe pulse due to noncollinear degenerate phase modulation induced by a femtosecond pump pulse were extracted from the set of inline digital holograms and analyzed. The absolute values of the nonlinear refractive index of both the sample glass substrate and the deposited layer of quantum dots were evaluated using the proposed technique. To characterize the inhomogeneous distribution of the samples’ nonlinear optical properties, we proposed plotting an optical nonlinearity map calculated as a local standard deviation of the diffraction pattern intensities induced by noncollinear degenerate phase modulation. Full article

(This article belongs to the Special Issue Digital Holography: Development and Application)

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29 pages, 4655 KiB

Open AccessReview

Roadmap on Digital Holography-Based Quantitative Phase Imaging

by Vinoth Balasubramani, Małgorzata Kujawińska, Cédric Allier, Vijayakumar Anand, Chau-Jern Cheng, Christian Depeursinge, Nathaniel Hai, Saulius Juodkazis, Jeroen Kalkman, Arkadiusz Kuś, Moosung Lee, Pierre J. Magistretti, Pierre Marquet, Soon Hock Ng, Joseph Rosen, Yong Keun Park and Michał Ziemczonok

J. Imaging 2021, 7(12), 252; https://doi.org/10.3390/jimaging7120252 - 26 Nov 2021

Cited by 57 | Viewed by 9485

Abstract

Quantitative Phase Imaging (QPI) provides unique means for the imaging of biological or technical microstructures, merging beneficial features identified with microscopy, interferometry, holography, and numerical computations. This roadmap article reviews several digital holography-based QPI approaches developed by prominent research groups. It also briefly [...] Read more.

(This article belongs to the Special Issue Digital Holography: Development and Application)

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22 pages, 7362 KiB

Open AccessArticle

Efficient Phase Unwrapping Architecture for Digital Holographic Microscopy

by Wen-Jyi Hwang, Shih-Chang Cheng and Chau-Jern Cheng

Sensors 2011, 11(10), 9160-9181; https://doi.org/10.3390/s111009160 - 27 Sep 2011

Cited by 10 | Viewed by 7743

Abstract

This paper presents a novel phase unwrapping architecture for accelerating the computational speed of digital holographic microscopy (DHM). A fast Fourier transform (FFT) based phase unwrapping algorithm providing a minimum squared error solution is adopted for hardware implementation because of its simplicity and robustness to noise. The proposed architecture is realized in a pipeline fashion to maximize through put of thecomputation. Moreover, the number of hardware multipliers and dividers are minimized to reduce the hardware costs. The proposed architecture is used as a custom user logic in a system on programmable chip (SOPC) for physical performance measurement. Experimental results reveal that the proposed architecture is effective for expediting the computational speed while consuming low hardware resources for designing an embedded DHM system. Full article

(This article belongs to the Section Physical Sensors)

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