Proteomic Determination of Low-Molecular-Weight Glutenin Subunit Composition in Aroona Near-Isogenic Lines and Standard Wheat Cultivars
Abstract
:1. Introduction
2. Results
2.1. Identification of LMW-GS Alleles Using RP-HPLC
2.2. Comparison of LMW-GSs among ‘Aroona’ and Its Near-Isogenic Lines Using 2-DGE
2.3. Identification of LMW-GSs Using LC–MS/MS Analysis
3. Discussion
3.1. Glu-A3 Alleles
3.2. Glu-B3 Alleles
3.3. Glu-D3 Alleles
4. Materials and Methods
4.1. Plant Materials
4.2. Glutenin Extraction
4.3. Two-Dimensional Gel Electrophoresis (2-DGE)
4.4. Identification of LMW-GSs Using UPLC–MS/MS
4.5. Separation of LMW-GSs Using RP-HPLC
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Line | Glu-A3 | Glu-B3 | Glu-D3 | Donor Parent |
---|---|---|---|---|
Aroona | c | b | c | Aroona |
ARIL 15-4 | a | b | c | Chinese spring |
ARIL 16-1 | b | b | c | Gabo |
ARIL 18-5 | d | b | c | Orca |
ARIL 19-2 | e | b | c | Lerma Rojo |
ARIL 20-1 | f | b | c | Bungulla |
ARIL 21-2 | c | a | c | Chinese spring |
ARIL 23-4 | c | c | c | Halberd |
ARIL 24-3 | c | d | c | Orca |
ARIL 27-6 | c | g | c | Millewa |
ARIL 28-4 | c | h | c | Sonalika |
ARIL 36-2 | c | b | b | Bungulla |
ARIL 35-1 | c | b | f | India 115 |
# Spot | Cultivars | Gene | MS/MS Identification | a Gene Haplotype | N-Terminal Sequence | # A.A | b Putative Corresponding Genes | |
---|---|---|---|---|---|---|---|---|
Accession No. (Identity) | Gene | |||||||
1 | AROONA | Glu-B3b | ACA63873 | GluB3-22 | MENSHIP | 349 | EU369721 (100%) | B3-621a |
2 | AROONA | Glu-B3b | ACA63873 | GluB3-22 | MENSHIP | 349 | EU369721 (100%) | B3-621a |
3 | AROONA | Glu-D3c | ABC84367 | GluD3-32 | IENSHIP | 334 | FJ755316 (100%) | D3-578a |
4 | AROONA | Glu-D3c | ABC84367 | GluD3-32 | IENSHIP | 334 | FJ755316 (100%) | D3-578a |
5 | AROONA | Glu-D3c | ABC84367 | GluD3-32 | IENSHIP | 334 | FJ755316 (100%) | D3-578a |
6 | AROONA | Glu-D3c | ABC84367 | GluD3-32 | IENSHIP | 334 | FJ755316 (100%) | D3-578a |
7 | ARIL 27-6 | Glu-B3g | ACA63857 | GluB3-23 | MENSHIP | 349 | EU369705 (100%) | B3-621b |
8 | ARIL 27-6 | Glu-B3g | ACA63857 | GluB3-23 | MENSHIP | 349 | EU369705 (100%) | B3-621b |
9 | ARIL 27-6 | Glu-B3g | ABY58126 | GluB3-15 | MENSHIP | 323 | EU369703 (100%) | B3-544 |
10 | ARIL 27-6 | Glu-B3g | ABY58126 | GluB3-15 | MENSHIP | 323 | EU369703 (100%) | B3-544 |
11 | ARIL 27-6 | Glu-B3g | ACZ59817 | - | MENSHIP | 324 | EU369703 (100%) | B3-544 |
12 | ARIL 36-2 | Glu-D3b | ABC84366 | GluD3-31 | MENSHIP | 344 | JX878006 (100%) | D3-578b |
Line | Alleles | a Active LMW-GS Genes (b Gene Name in Chinese Wheat Germplasm) | |||||
---|---|---|---|---|---|---|---|
At Glu-3A | |||||||
ARIL15-4 | Glu-A3a | GluA3-11 (A3-620) | |||||
ARIL16-1 | Glu-A3b | GluA3-12 (A3-643) | |||||
Aroona | Glu-A3c | GluA3-13 (A3-620) | |||||
ARIL18-5 | Glu-A3d | GluA3-23 (A3-402) | - (A3-568) | GluA3-4 (A3-662) | |||
ARIL19-2 | Glu-A3e | GluA3-15/17 (A3-646) | |||||
ARIL20-1 | Glu-A3f | GluA3-16 (A-573) | |||||
At Glu-3B | |||||||
ARIL21-2 | Glu-B3a | GluB3-44 (B3-530a) | GluB3-11 (B3-593) | GluB3-21 (B3-624) | |||
Aroona | Glu-B3b | GluB3-43 (B3-530b) | GluB3-12 (B3-607) | GluB3-22 (B3-621a) | |||
ARIL23-4 | Glu-B3c | GluB3-44 (B3-530a) | GluB3-34 (B3-688a) | ||||
ARIL24-3 | Glu-B3d | GluB3-44 (B3-530a) | GluB3-31–33 (B3-688b) | ||||
ARIL27-6 | Glu-B3g | GluB3-41 (B3-530c) | GluB3-15 (B3-544) | GluB3-23 (B3-621b) | |||
ARIL28-4 | Glu-B3h | GluB3-43 (B3-530b) | GluB3-61–64 (B3-688c) | ||||
At Glu-3D | |||||||
ARIL36-2 | Glu-D3b | GluD3-6 (D3-385) | GluD3-4 (D3-394) | GluD3-22 (D3-441) | GluD3-12 (D3-528) | GluD3-5 (D3-575) | GluD3-31 (D3-578b) |
Aroona | Glu-D3c | GluD3-6 (D3-385) | GluD3-4 (D3-394) | GluD3-23 (D3-432) | GluD3-12 (D3-528) | GluD3-5 (D3-575) | GluD3-32 (D3-578a) |
ARIL35-1 | Glu-D3f | GluD3-6 (D3-385) | GluD3-4 (D3-394) | GluD3-22 (D3-441) | GluD3-12 (D3-528) | GluD3-5 (D3-575) | GluD3-31 (D3-578b) |
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Cho, K.; Jang, Y.-R.; Lim, S.-H.; Altenbach, S.B.; Gu, Y.Q.; Simon-Buss, A.; Lee, J.-Y. Proteomic Determination of Low-Molecular-Weight Glutenin Subunit Composition in Aroona Near-Isogenic Lines and Standard Wheat Cultivars. Int. J. Mol. Sci. 2021, 22, 7709. https://doi.org/10.3390/ijms22147709
Cho K, Jang Y-R, Lim S-H, Altenbach SB, Gu YQ, Simon-Buss A, Lee J-Y. Proteomic Determination of Low-Molecular-Weight Glutenin Subunit Composition in Aroona Near-Isogenic Lines and Standard Wheat Cultivars. International Journal of Molecular Sciences. 2021; 22(14):7709. https://doi.org/10.3390/ijms22147709
Chicago/Turabian StyleCho, Kyoungwon, You-Ran Jang, Sun-Hyung Lim, Susan B. Altenbach, Yong Q. Gu, Annamaria Simon-Buss, and Jong-Yeol Lee. 2021. "Proteomic Determination of Low-Molecular-Weight Glutenin Subunit Composition in Aroona Near-Isogenic Lines and Standard Wheat Cultivars" International Journal of Molecular Sciences 22, no. 14: 7709. https://doi.org/10.3390/ijms22147709