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Volume 26
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Current Issues in Molecular Biology is published by MDPI from Volume 43 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Caister Press.
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Review

Type III CRISPR/Cas System: Introduction and Its Application for Genetic Manipulations

by
Tao Liu
1,*,
Saifu Pan
1,
Yingjun Li
1,2,
Nan Peng
1 and
Qunxin She
2
1
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China
2
Archaea Centre, Department of Biology, University of Copenhagen, Copenhagen, Denmark
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2018, 26(1), 1-14; https://doi.org/10.21775/cimb.026.001
Submission received: 1 June 2017 / Revised: 2 July 2017 / Accepted: 5 August 2017 / Published: 7 September 2017

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes provide adaptive immunity against invasion of foreign nucleic acids in archaea and bacteria. The system functions in three distinct stages: adaptation, biogenesis, and interference. CRISPR-Cas systems are currently classified into at least five different types, each with a signature protein among which Type III systems exhibit a dual DNA/RNA interference activity. Structures of a few Type III surveillance complexes have been determined: they are composed of several different subunits and exhibit striking architectural similarities to Type I surveillance complexes. Here, we review the genetic, biochemical, and structural studies concerning CRISPR-Cas Type III systems and discuss their application for genetic manipulations, including genome engineering and gene silencing.

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MDPI and ACS Style

Liu, T.; Pan, S.; Li, Y.; Peng, N.; She, Q. Type III CRISPR/Cas System: Introduction and Its Application for Genetic Manipulations. Curr. Issues Mol. Biol. 2018, 26, 1-14. https://doi.org/10.21775/cimb.026.001

AMA Style

Liu T, Pan S, Li Y, Peng N, She Q. Type III CRISPR/Cas System: Introduction and Its Application for Genetic Manipulations. Current Issues in Molecular Biology. 2018; 26(1):1-14. https://doi.org/10.21775/cimb.026.001

Chicago/Turabian Style

Liu, Tao, Saifu Pan, Yingjun Li, Nan Peng, and Qunxin She. 2018. "Type III CRISPR/Cas System: Introduction and Its Application for Genetic Manipulations" Current Issues in Molecular Biology 26, no. 1: 1-14. https://doi.org/10.21775/cimb.026.001

APA Style

Liu, T., Pan, S., Li, Y., Peng, N., & She, Q. (2018). Type III CRISPR/Cas System: Introduction and Its Application for Genetic Manipulations. Current Issues in Molecular Biology, 26(1), 1-14. https://doi.org/10.21775/cimb.026.001

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