Synergistic Effect of Dietary Betaines on SIRT1-Mediated Apoptosis in Human Oral Squamous Cell Carcinoma Cal 27
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. Effect of Milk Betaines on Cell Viability, Proliferation and Sirtuins
2.2. Effects of Pure δVB and γBB on Cancer Cell Proliferation
2.3. Cell Cycle Phase Modulation
2.4. Apoptotic Cell Death
2.5. SIRT1 Activation
2.6. ROS Levels
2.7. SIRT1 Mediates Betaine Anticancer Activity
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Treatments
4.2. Cell Proliferation and Viability Assays
4.3. Mitochondrial ROS Evaluation
4.4. Measurement of Extracellular H2O2 Using Amplex Red
4.5. Confocal Laser Scanning Microscopy
4.6. SIRT1 Enzyme Assay
4.7. Cell Cycle Distribution Analysis
4.8. Apoptotic Cells Analysis
4.9. SIRT1 Gene Silencing
4.10. Preparation of Cell Lysates and Western Blotting Analysis
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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D’Onofrio, N.; Mele, L.; Martino, E.; Salzano, A.; Restucci, B.; Cautela, D.; Tatullo, M.; Balestrieri, M.L.; Campanile, G. Synergistic Effect of Dietary Betaines on SIRT1-Mediated Apoptosis in Human Oral Squamous Cell Carcinoma Cal 27. Cancers 2020, 12, 2468. https://doi.org/10.3390/cancers12092468
D’Onofrio N, Mele L, Martino E, Salzano A, Restucci B, Cautela D, Tatullo M, Balestrieri ML, Campanile G. Synergistic Effect of Dietary Betaines on SIRT1-Mediated Apoptosis in Human Oral Squamous Cell Carcinoma Cal 27. Cancers. 2020; 12(9):2468. https://doi.org/10.3390/cancers12092468
Chicago/Turabian StyleD’Onofrio, Nunzia, Luigi Mele, Elisa Martino, Angela Salzano, Brunella Restucci, Domenico Cautela, Marco Tatullo, Maria Luisa Balestrieri, and Giuseppe Campanile. 2020. "Synergistic Effect of Dietary Betaines on SIRT1-Mediated Apoptosis in Human Oral Squamous Cell Carcinoma Cal 27" Cancers 12, no. 9: 2468. https://doi.org/10.3390/cancers12092468