Regulation of Cell Viability, p53 Promoter Activity, and Expression of Interleukin-8, Matrixmetalloproteinase-1 and Tissue Inhibitor of Matrixmetalloproteinase-1 in Non-Irradiated or UV-Irradiated Fibroblasts and Melanoma Cells
Abstract
:1. Introduction
2. Methods
2.1. Cell Culture
2.2. Cell Viability
2.2.1. Promoter Activities: p53, IL-8
2.2.2. Protein Levels: IL-8, MMP-1 and TIMP-1
2.3. Data Analysis
3. Results
3.1. Effect of Hydrocortisone on IL-8 Promoter Activity and Protein Levels in Non-Irradiated or UV-Irradiated Fibroblasts, and Melanoma Cells
3.2. Effect of Hydrocortisone on MMP-1 and TIMP-1 Protein Levels in Non-Irradiated or UV-Irradiated Fibroblasts, and Melanoma Cells
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Philips, N.; Pena, J.; Almeshni, N.; Gonzalez, S. Regulation of Cell Viability, p53 Promoter Activity, and Expression of Interleukin-8, Matrixmetalloproteinase-1 and Tissue Inhibitor of Matrixmetalloproteinase-1 in Non-Irradiated or UV-Irradiated Fibroblasts and Melanoma Cells. Targets 2024, 2, 287-294. https://doi.org/10.3390/targets2040016
Philips N, Pena J, Almeshni N, Gonzalez S. Regulation of Cell Viability, p53 Promoter Activity, and Expression of Interleukin-8, Matrixmetalloproteinase-1 and Tissue Inhibitor of Matrixmetalloproteinase-1 in Non-Irradiated or UV-Irradiated Fibroblasts and Melanoma Cells. Targets. 2024; 2(4):287-294. https://doi.org/10.3390/targets2040016
Chicago/Turabian StylePhilips, Neena, Jonathan Pena, Nadyeh Almeshni, and Salvador Gonzalez. 2024. "Regulation of Cell Viability, p53 Promoter Activity, and Expression of Interleukin-8, Matrixmetalloproteinase-1 and Tissue Inhibitor of Matrixmetalloproteinase-1 in Non-Irradiated or UV-Irradiated Fibroblasts and Melanoma Cells" Targets 2, no. 4: 287-294. https://doi.org/10.3390/targets2040016
APA StylePhilips, N., Pena, J., Almeshni, N., & Gonzalez, S. (2024). Regulation of Cell Viability, p53 Promoter Activity, and Expression of Interleukin-8, Matrixmetalloproteinase-1 and Tissue Inhibitor of Matrixmetalloproteinase-1 in Non-Irradiated or UV-Irradiated Fibroblasts and Melanoma Cells. Targets, 2(4), 287-294. https://doi.org/10.3390/targets2040016