Early Detection of Phototrophic Biofilms in the Polychrome Panel, El Castillo Cave, Spain
Abstract
:1. Introduction
2. Materials and Methods
2.1. The Site
2.2. Cave Monitoring
2.3. Sampling Site
2.4. Culture of Natural Samples
2.5. Extraction of Nucleic Acids
2.6. Sequencing
2.7. Bioinformatics
3. Results
3.1. Main Seasonal Pattern of Cave Environmental Conditions
3.2. Microbial Community Composition
3.3. Prokaryotes Community Structure
3.4. Eukaryotes Community Structure
4. Discussion
4.1. Microclimate Disruption by Daily Visitors and Cave Lighting
4.2. Prokaryota
4.3. Eukaryota
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Jurado, V.; Gonzalez-Pimentel, J.L.; Fernandez-Cortes, A.; Martin-Pozas, T.; Ontañon, R.; Palacio, E.; Hermosin, B.; Sanchez-Moral, S.; Saiz-Jimenez, C. Early Detection of Phototrophic Biofilms in the Polychrome Panel, El Castillo Cave, Spain. Appl. Biosci. 2022, 1, 40-63. https://doi.org/10.3390/applbiosci1010003
Jurado V, Gonzalez-Pimentel JL, Fernandez-Cortes A, Martin-Pozas T, Ontañon R, Palacio E, Hermosin B, Sanchez-Moral S, Saiz-Jimenez C. Early Detection of Phototrophic Biofilms in the Polychrome Panel, El Castillo Cave, Spain. Applied Biosciences. 2022; 1(1):40-63. https://doi.org/10.3390/applbiosci1010003
Chicago/Turabian StyleJurado, Valme, Jose Luis Gonzalez-Pimentel, Angel Fernandez-Cortes, Tamara Martin-Pozas, Roberto Ontañon, Eduardo Palacio, Bernardo Hermosin, Sergio Sanchez-Moral, and Cesareo Saiz-Jimenez. 2022. "Early Detection of Phototrophic Biofilms in the Polychrome Panel, El Castillo Cave, Spain" Applied Biosciences 1, no. 1: 40-63. https://doi.org/10.3390/applbiosci1010003