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Proceeding Paper

Control of Carrot Seed-Borne Pathogens by Aromatic Plants Distillates †

Laboratory of Plant Protection, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Babtai, LT-54333 Kaunas, Lithuania
Author to whom correspondence should be addressed.
Presented at the 1st International Electronic Conference on Plant Science, 1–15 December 2020; Available online:
Biol. Life Sci. Forum 2021, 4(1), 29;
Published: 30 November 2020
(This article belongs to the Proceedings of The 1st International Electronic Conference on Plant Science)


Global warming, pollution problems, and the demand for sustainable food production forced farmers and scientists to search for new solutions in biological plant protection. The usage of natural renewable sources around us seems to be an alternative. Compounds isolated from plants and distinguished with antifungal properties can be used to protect vegetables from the seed-borne pathogens. The study aimed to elucidate the ability of Juniperus communis L., Hyssopus officinalis L. and Thymus vulgaris L. essential oils to control the carrot seed-borne pathogen Alternaria spp. The agar-plate method was used for carrot seed infestation with micromycetes. Essential oils extracted from common juniper, hyssop and thyme were then separately mixed with potato dextrose agar media at different concentrations and the antifungal activity of each oil tested in vitro. The results revealed that the T. vulgaris essential oil (200–1000 μL L−1) significantly inhibited Alternaria spp. growth. The H. officinalis essential oil promoted seed-borne pathogens growth on the second and fifth days of the evaluation compared to the control; however, the concentration of 400 μL L−1 showed little suppression of micromycete development 7 days after inoculation. The in vitro experiments indicated that 600 μL L−1 of J. communis essential oil could control seed-borne pathogen viability. Overall, thyme essential oil expressed a high potential for application in biofungicide formulations.

1. Introduction

The carrot (Daucus carota L.) is one of the most crucial root vegetables in the family Apiaceae, which is cultivated worldwide. Current studies are mainly focused on nutrient content, breeding, cultivation, increasing yield, tissue culture, and regulating carotenoid synthesis [1,2,3]. However, very often, the cultivation of vegetables is faced with various diseases caused by microorganisms living in the soil. Moreover, the quality of the seeds themselves and contamination with micromycetes are rarely considered. Seeds take a massive part of crop production and are vital for plant associations with microorganisms. Seed-borne pathogens infect seeds and cause growth disorders of infected crops. The Alternaria genus is well known as causal of destructive diseases of carrots, parsnips, celery, and parsley with symptoms such as seedling death, petiole base blackening, leaf death, black rot of the crown, and stored roots [4]. In addition to these features, this pathogen also adversely affects seed germination [5]. Seed-borne diseases can be managed by various strategies including chemical, cultural and technological methods [6]. In the past few decades, chemicals are widely used for treatment as a potent approach toward disease control, and the usage of chemical fungicides added further possibilities to it [7,8,9,10]. Nevertheless, pesticides pose distinct risks to the organic production of carrot seeds, because the use of effective chemical fungicides to resist foliar and leaf blight is not allowed. Additionally, their non-target environmental impact like the development of pathogen resistance, hazards to human health and other living organisms, pesticide residues in the environment, and agricultural products has led to the search for alternative methods to control and make agriculture more sustainable [11,12].
Medicinal and aromatic plants are natural renewable sources full of bioactive compounds. Essential oils extracted from them are widely studied for their antioxidant, antibacterial, antifungal, immunomodulatory, and also anti-inflammatory properties [13,14,15,16]. Due to these features, they can be applied to innovative plant protection; for example, clove, mint, and oregano essential oils inhibited the growth of fungal species belonging to Acremonium, Alternaria, Arhrobotrys, Aspergillus, Cladosporium, Epicoccum, Fusarium, Penicillium, Rhizopus, Trichoderma, and Ulocladium genera on wheat seeds [17]. The high antifungal effectiveness of palmarosa, lemongrass, and geranium essential oils has already been observed against seed-borne fungi Cochliobolus miyabeanus Ito & Kuribayashi and Fusarium verticillioides (Sacc.) Nirenberg of rice [18]. The literature review revealed that there has been comparatively less research regarding environmentally friendly ways to prevent fungal infections of carrot seeds; especially, there is a lack of studies on the antifungal activity of common juniper, hyssop, and thyme essential oils against Alternaria spp. Therefore, we aimed to evaluate the ability of essential oils of Juniperus communis L., Hyssopus officinalis L. and Thymus vulgaris L. to control the carrot seed-borne pathogen Alternaria spp.

2. Experiments

2.1. Seed Preparation

For the research, carrot seed cultivar ‘Svalia’ was used, which was obtained from the Lithuanian Research Centre for Agriculture and Forestry Institute of Horticulture (LAMMC IH) Department of Vegetable Breeding and Technology. The disinfection of vegetable seeds was carried out by soaking them in 70% ethanol for 5 min and then rinsing them in sterile water three times for 5 min. After this, seeds were dried for 5–10 min in laminar flow to evaporate water from the surface of the seeds. This procedure removes extraneous microorganisms from the outside of the husk and allows to determine the internal infestation of seeds with pathogens.

2.2. Essential Oils Production

H. officinalis and T. vulgaris plants were grown in the LAMMC IH experimental fields. Following the methods of essential oils extraction, the necessary essential oils (EOs) were separately isolated by hydrodistillation, in which the naturally dried plant material is boiled in water under normal atmospheric pressure for 2 h [19]. The EO of J. communis was obtained (UAB Naujoji Barmune).

2.3. Medium Preparation

Potato dextrose agar (PDA) medium (Sigma-Aldrich) consisting of 4 g L−1 potato extract, 15 g L−1 agar, and 20 g L−1 dextrose was used for this study. Potato extract is a source of nutrients that promotes fungal sporulation and pigment production, and dextrose acts as a growth stimulator [20]. The medium with the acidity of 5.6 ± 0.2 was sterilised in a high-pressure autoclave for 20 min at 121 °C. After autoclaving, different concentrations of 200, 400, 600, 800 and 1000 μL L−1 of each EO were added to the medium after cooling it to 45 °C, mixed, and poured into sterilised Petri dishes, allowing them to solidify. A control treatment was created without EO.

2.4. Essential Oils Effect on Pathogens

The research was carried out at the LAMMC IH Laboratory of Plant Protection. Using the agar-plate method [21], prepared surface-sterilised carrot seeds were arranged in a square shape (5 rows and 5 columns) on each Petri dish with PDA and the appropriate concentration of EO, as shown in Figure 1.
Each study treatment consisted of 24 plates (4 replicates). Dishes were incubated at 22 ± 2 °C temperature in the dark for up to 10 days [22]. Seed internal disease assessment was performed after 2, 5, and 7 days. The number of fungal and bacterial colonies on the seeds was counted in each plate and the percentage of Alternaria spp. settlements in the treatment were calculated. Essential oil effect on pathogens was evaluated according to the infection rate using the formula below (1) [5]. Lower infection rate described the more effective activity to control seed-borne pathogens.
Alternaria spp. infected seeds rate (%) = Number of seeds infected by Alternaria spp. × 100/Total number of infected seeds.
Types of fungus were determined visually and microscopically based on cultural and morphological characteristics typical to the colonies [10,23].

2.5. Statistics

Experimental data were analysed by SAS Enterprise Guide 7.1 program (SAS Institute Inc., Cary, NC, USA). The analysis of variance (ANOVA) procedure was processed. Other calculations were performed using Microsoft Excel.

3. Results

The antifungal activity of H. officinalis, T. vulgaris and J. communis EOs was investigated on PDA under different concentrations in vitro. The incidence of Alternaria spp. under the influence of T. vulgaris EO is presented in Figure 2. It can be seen that the growth of seed-borne pathogens was significantly inhibited (p < 0.05) by all used concentrations of this EO. No fungal germs were noticed in all treatments after 2 days. However, in the control treatment, the spread of Alternaria spp. reached 36% of all grown fungi and bacteria after 5 days and increased to 38% after 7 days.
The incidence of Alternaria spp. on seeds under the influence of H. officinalis EO is presented in Figure 3. Assessment of carrot seed lesions at 400 and 1000 μL L−1 concentrations of this EO revealed that Alternaria spp. damaged 1% of seeds after 2 days. At the next evaluation, after 5 days, there was 39% prevalence of this fungus in the control treatment, while with all used applications of EOs it was higher: 800 μL L−1—48%, 400 μL L−1—52%, 200 μL L−1—63%, 1000 μL L−1—64%, and 600 μL L−1—74% contaminated seeds. H. officinalis EO showed the highest inhibition at 600 μL L−1 after 7 days, causing a decrease in the percentage of infected seeds. However, 200 μL L−1, 800 μL L−1, and 1000 μL L−1 of EO did not inhibit fungal growth—the number of micromycetes increased to 75%, 64%, and 74%, respectively. Thus, H. officinalis EO even promoted the development of the fungus more than inhibited it.
The prevalence of Alternaria spp. under the influence of J. communis EO is presented in Figure 4. The assay revealed that J. communis EO had a weak antifungal activity against fungi of the genus Alternaria after 2 days. Still, the best effect was shown by a concentration of 600 μL L−1 (16%). The spread of micromycetes was slightly higher (31–32%) with 200 μL L−1 and 600–1000 μL L−1 of J. communis EO than the controls (29%) after 5 days. After 7 days, the number of pathogenic fungi in carrot seeds did not change significantly. Nevertheless, 400 μL L−1 of this plant distillate exhibited the best fungal incidence inhibition (22–23%) at 5 and 7 days of the experiment.
Analysis of data showed that these distillates had an unequal effect on the health of carrot seeds and Alternaria spp. The T. vulgaris EO (200–1000 μL L−1) inhibited the growth of micromycetes most significantly, but 400 μL L−1 of J. communis EO also had a modest antifungal effect.

4. Discussion

Green issues and potential threats to human health arising from the long-term use of chemical fungicides require innovative plant protection solutions [11,12]; also, due to infections of the seeds by Alternaria spp., the germination rate decreases [5]. Thus, screening for suitable biofungicides and optimizing their applications is essential. This study provided new data about in vitro inhibition effects of medicinal and aromatic plant-based substances against carrot seed-borne pathogens Alternaria spp.
The T. vulgaris EO concentration 200–1000 μL L−1 significantly reduced fungus incidence when it was compared to the untreated control. There are several reports about antibacterial and antifungal activities of T. vulgaris oil components and some other experiments also revealed that this oil might be effectively used to control Alternaria spp. on carrot seeds [24,25,26,27]. Dorna and Szopińska [25], using a different methodology, found that the percentage of seeds free of fungi increased and the incidence of Alternaria alternata (Fr.) Keissl. in cultivar ‘Flakkese 2′ seeds decreased. However, the opposite effect was found in the cultivar ‘Amsterdam 3′ seeds: T. vulgaris EO favoured the growth of Alternaria radicina Meier, Drechsler & E.D. Eddy and Alternaria dauci (J.G. Kühn) J.W. Groves & Skolko, but the percentage of infected seeds was only 0.5. It is known that some microorganisms are stimulated by antimicrobial agents and use EO as a carbon energy source [26]; this may have been the case for the A. radicina examined in Dorna and Szopińska study. Koch et al. [27] confirmed the high antifungal activity of T. vulgaris EO against Alternaria species when used seeds (variety ‘Laguna’) were stirred for 4 h in 1% oil emulsion. However, the authors emphasise that due to inherent oil phytotoxicity, the choice of the optimal concentration is critical, and pre-testing is recommended. Our results contribute to the findings of Riccioni and Orzali [28], whose study showed promising prospects for a source of natural plant EOs and that T. vulgaris EO concentrations of 0.05%, 0.1%, 0.25%, 0.5%, 1% had an apparent reducing effect on A. dauci fungal growth in vitro.
In our study, it was found that J. communis EO exhibited the highest Alternaria spp. inhibition at 400 μL L−1. Meanwhile, investigations are determining the ability of the J. communis to inhibit the development of seed-borne and soil-borne pathogens, but not in the Alternaria genus. For example, in Zabka et al. [29] research, J. communis EO exhibited average efficacy at 1 μL mL−1 concentration on other pathogens such as Fusarium oxysporum Schlechtendahl, F. verticillioides (Sacc.) Nirenberg, Penicillium brevicompactum Dierckx, P. expansum Link, Aspergillus flavus Link, and A. fumigates Fresenius, the effect on Alternaria spp. have not been studied. Nevertheless, T. vulgaris were determined as the most effective against the growth of target fungal species. Additionally, while screening the methanolic extract of J. communis, good antimicrobial activity against the Candida albicans (C. P. Robin) Berkhout, A. niger, and A. flavus fungi was observed [30]. Menghani and Sharma results revealed the maximum activity against A. niger and A. flavus. Additionally, this EO was effective against phytopathogenic bacteria.
Analysing the antifungal effect of H. officinalis EO, in our assay, it did not inhibit fungal development and even stimulated it compared to the control treatment. However, Fraternale and colleagues [31] have shown significant antifungal activity against 13 strains of phytopathogenic fungi. Reports indicated that 100% inhibition for all pathogens as well as Alternaria solani Sorauer was achieved with 1400 and 1600 μL mL−1 of oil. According to the authors, this may have been due to higher levels of active substances: pinocamphone, isopinocamphone, linalool, and camphor. Based on data from other scientists, it can be assumed that too low concentrations of this oil were used. Still, there are many investigations on H. officinalis antimicrobial features with other microorganisms, where this EO indicates undoubted prospects [32].
To conclude, EOs of J. communis, H. officinalis, and T. vulgaris showed moderate ability to control the seed-borne pathogens. Results demonstrated that T. vulgaris EO had a significant reducing effect on fungal pathogens, confirming what is already reported in the literature. However, because the in vitro effects did not always provide a good effect for their in vivo performances, additional studies are necessary to verify the effectiveness in field conditions as seed treatment and their possible phytotoxicity on the plant or seed material. Furthermore, T. vulgaris EO is promising against carrot seed-borne pathogen Alternaria spp.

5. Conclusions

T. vulgaris EO concentrations of 200–1000 μL L−1 revealed the potential to suppress the prevalence of Alternaria spp., it significantly inhibited fungal growth, while J. communis and H. officinalis EO inhibition was dose-dependent and less powerful. The T. vulgaris plant can be considered as a potential source of biofungicide to the chemical products that are currently used to prevent and control seed-borne diseases and could be used in agriculture for safe and nature-friendly seed-treatments.

Author Contributions

Conceptualization, N.R. and A.V.; Data curation, S.L.; Formal analysis, S.L.; Investigation, S.L.; Methodology, S.L., N.R. and A.V.; Resources, N.R.; Software, S.L.; Supervision, N.R. and A.V.; Validation, N.R.; Visualization, S.L.; Writing—original draft, S.L.; Writing—review & editing, S.L., N.R. and A.V. All authors have read and agreed to the published version of the manuscript.


This research received no external funding.


Not applicable.

Conflicts of Interest

The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.


The following abbreviations are used in this manuscript:
LAMMCLithuanian Research Centre for Agriculture and Forestry
IHInstitute of Horticulture
PDAPotato dextrose agar
EOsEssential oils
ANOVAAnalysis of variance


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Figure 1. Arrangement of 25 carrot seeds.
Figure 1. Arrangement of 25 carrot seeds.
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Figure 2. The seed infection with Alternaria spp. under the influence of different concentrations of T. vulgaris essential oil after 2, 5, and 7 days.
Figure 2. The seed infection with Alternaria spp. under the influence of different concentrations of T. vulgaris essential oil after 2, 5, and 7 days.
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Figure 3. The seed infection with Alternaria spp. under the influence of different concentrations of hyssop essential oil after 2, 5, and 7 days.
Figure 3. The seed infection with Alternaria spp. under the influence of different concentrations of hyssop essential oil after 2, 5, and 7 days.
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Figure 4. The seed infection with Alternaria spp. under the influence of different concentrations of common juniper essential oil after 2, 5, and 7 days.
Figure 4. The seed infection with Alternaria spp. under the influence of different concentrations of common juniper essential oil after 2, 5, and 7 days.
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Lukošiūtė, S.; Rasiukevičiūtė, N.; Valiuškaitė, A. Control of Carrot Seed-Borne Pathogens by Aromatic Plants Distillates. Biol. Life Sci. Forum 2021, 4, 29.

AMA Style

Lukošiūtė S, Rasiukevičiūtė N, Valiuškaitė A. Control of Carrot Seed-Borne Pathogens by Aromatic Plants Distillates. Biology and Life Sciences Forum. 2021; 4(1):29.

Chicago/Turabian Style

Lukošiūtė, Simona, Neringa Rasiukevičiūtė, and Alma Valiuškaitė. 2021. "Control of Carrot Seed-Borne Pathogens by Aromatic Plants Distillates" Biology and Life Sciences Forum 4, no. 1: 29.

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