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Biology and Life Sciences Forum
Volume 16
Issue 1
10.3390/IECHo2022-12507
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Abstract

Assessment of the Effect of Outer Membrane Vesicles of Endophytic Bacteria on the Growth and Physiological Response of Arabidopsis thaliana †

by
Małgorzata Pawlik
*,
Małgorzata Rudnicka
,
Iryna Bondaruk
,
Katarzyna Kasperkiewicz
,
Magdalena Noszczyńska
,
Monika Malicka
,
Piotr Siupka
and
Zofia Piotrowska-Seget
Institute for Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, The University of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice, Poland
*
Author to whom correspondence should be addressed.
†
Presented at the 1st International Electronic Conference on Horticulturae, 16–30 April 2022; Available online: https://sciforum.net/event/IECHo2022.
Biol. Life Sci. Forum 2022, 16(1), 36; https://doi.org/10.3390/IECHo2022-12507
Published: 15 April 2022
(This article belongs to the Proceedings of The 1st International Electronic Conference on Horticulturae)
Versions Notes

Abstract

The world faces the challenge of producing a sufficient amount of food for the constantly growing human population in a sustainable manner. It means the usage of pesticides and fertilizers with minimized adverse effects on the environment and the wide application of beneficial microorganisms. Bacteria produce outer membrane vesicles (OMVs) that might affect plant development. The aim of the study was to evaluate the impact of OMVs on the Arabidopsis thaliana condition. The experiments were conducted with endophytic bacteria. To obtain OMVs, specific protocols for their isolation have been developed. OMVs’ morphology, size, and surface charge have been studied with the use of SEM and TEM. The OMVs were added to the surface of sterilized seeds of A. thaliana that were grown on Murashige and Skoog agar medium for 14–21 days. To assess the impact of OMVs on plants, the rate of seeds germination, biomass, and the length of the shoots and roots were measured. Moreover, the level of oxidative stress and the activity of the antioxidant enzymes in the plants were evaluated. The experiments conducted so far have shown that OMVs produced by tested strains had a great ability to induce the germination and growth of seedlings. The OMVs that originated from the tested bacteria differently influenced the length of plant roots. Serratia sp. OMVs inhibited the growth of A. thaliana seedlings, whereas OMVs released by Pseudomonas sp. stimulated it. Furthermore, OMVs produced by both bacterial strains had a great effect on the activity of oxidative plant enzymes. The knowledge concerning plant response to OMVs is limited. Therefore, the preliminary studies seem to be important to obtain knowledge that may be applicable in the development of new natural compounds that can be used as plant growth stimulators.
Keywords: outer membrane vesicles (OMVs); endophytic bacteria; Arabidopsis thaliana; plant response outer membrane vesicles (OMVs); endophytic bacteria; Arabidopsis thaliana; plant response

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MDPI and ACS Style

Pawlik, M.; Rudnicka, M.; Bondaruk, I.; Kasperkiewicz, K.; Noszczyńska, M.; Malicka, M.; Siupka, P.; Piotrowska-Seget, Z. Assessment of the Effect of Outer Membrane Vesicles of Endophytic Bacteria on the Growth and Physiological Response of Arabidopsis thaliana. Biol. Life Sci. Forum 2022, 16, 36. https://doi.org/10.3390/IECHo2022-12507

AMA Style

Pawlik M, Rudnicka M, Bondaruk I, Kasperkiewicz K, Noszczyńska M, Malicka M, Siupka P, Piotrowska-Seget Z. Assessment of the Effect of Outer Membrane Vesicles of Endophytic Bacteria on the Growth and Physiological Response of Arabidopsis thaliana. Biology and Life Sciences Forum. 2022; 16(1):36. https://doi.org/10.3390/IECHo2022-12507

Chicago/Turabian Style

Pawlik, Małgorzata, Małgorzata Rudnicka, Iryna Bondaruk, Katarzyna Kasperkiewicz, Magdalena Noszczyńska, Monika Malicka, Piotr Siupka, and Zofia Piotrowska-Seget. 2022. "Assessment of the Effect of Outer Membrane Vesicles of Endophytic Bacteria on the Growth and Physiological Response of Arabidopsis thaliana" Biology and Life Sciences Forum 16, no. 1: 36. https://doi.org/10.3390/IECHo2022-12507

APA Style

Pawlik, M., Rudnicka, M., Bondaruk, I., Kasperkiewicz, K., Noszczyńska, M., Malicka, M., Siupka, P., & Piotrowska-Seget, Z. (2022). Assessment of the Effect of Outer Membrane Vesicles of Endophytic Bacteria on the Growth and Physiological Response of Arabidopsis thaliana. Biology and Life Sciences Forum, 16(1), 36. https://doi.org/10.3390/IECHo2022-12507

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