Immunomodulatory Properties of Polyphenol-Rich Sugarcane Extract on Human Monocytes
Abstract
:1. Introduction
2. Results
2.1. PRSE Modulates the Expression of Cell Surface Markers in U937 Monocyte Cells
2.2. PRSE Alters the Production of Immunomodulatory Cytokines
2.3. Immunomodulatory Actions of PRSE Are Not Mediated by NF-κB
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. U937 Cell Culture
4.3. U937 Cell Differentiation and Proinflammatory Activation
4.4. Treatment Groups
4.5. Cell Surface Marker Analysis
4.6. Cytokine Analysis
4.7. NF-κB ELISA
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Antibody | Fluorochrome | Concentration |
---|---|---|
CD11b | PE | 1:400 |
CD83 | AF 488 | 1:400 |
CD14 | BV 421 | 1:200 |
CD86 | AF 488 | 1:400 |
CD16 | PE | 1:400 |
CD206 | PE-Cy7 | 1:200 |
CD40 | AF 488 | 1:400 |
CD80 | AF 488 | 1:400 |
MHCI | BV 510 | 1:200 |
MHCII | BV 510 | 1:200 |
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Feehan, J.; Prakash, M.D.; Stojanovska, L.; Flavel, M.R.; Kitchen, B.; Apostolopoulos, V. Immunomodulatory Properties of Polyphenol-Rich Sugarcane Extract on Human Monocytes. Biologics 2021, 1, 211-221. https://doi.org/10.3390/biologics1020013
Feehan J, Prakash MD, Stojanovska L, Flavel MR, Kitchen B, Apostolopoulos V. Immunomodulatory Properties of Polyphenol-Rich Sugarcane Extract on Human Monocytes. Biologics. 2021; 1(2):211-221. https://doi.org/10.3390/biologics1020013
Chicago/Turabian StyleFeehan, Jack, Monica D. Prakash, Lily Stojanovska, Matthew Roland Flavel, Barry Kitchen, and Vasso Apostolopoulos. 2021. "Immunomodulatory Properties of Polyphenol-Rich Sugarcane Extract on Human Monocytes" Biologics 1, no. 2: 211-221. https://doi.org/10.3390/biologics1020013