The Characterization and Differentiation of Recombinant Lumpy Skin Disease Isolates Using a Region within ORF134
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sequences and Primer Design
2.2. Samples
2.3. DNA Extraction and PCR
2.4. Sequence Analysis
3. Results
3.1. Locus Identification
3.2. Assay Validation
4. Discussion
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
Ethics Declarations
References
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Isolate | Cluster | GenBank Accession Number | Reference |
---|---|---|---|
Neethling vaccine LW1959 | 1.1 | AF409138 | [33] |
KSGPO-240 vaccine | 1.2 | AF325528 | [34] |
Saratov Russia 2017 | 2.1 | MH646674 | [17] |
Udmutiya Russia 2019 | 2.2 | MT134042 | [22] |
Konstanay Kazakhstan 2018 | 2.3 | MT992618 | None |
Tyumen Russia 2019 | 2.4 | OL542833 | [35] |
GD01 China 2020 | 2.5 | MW355944 | [36] |
Dagestan Russia 2015 | 1.2 | MH893760 | [17] |
Position within the 705 bp Amplicon | Neethling Vaccine LW1959 | KSGPO-240 Vaccine | Dagestan Russia 2015 | Saratov Russia 2017 | Udmurtiya Russia 2019 | Kostanay Kazakhstan 2018 | Tyumen Russia 2019 | GD01 China 2020 |
---|---|---|---|---|---|---|---|---|
Cluster | 1.1 | 1.2 | 1.2 | 2.1 | 2.2 | 2.3 | 2.4 | 2.5 |
34 | T | C | C | C | T | C | C | T |
39 | T | C | C | C | T | C | C | T |
48 | A | G | G | G | A | G | G | A |
186 | G | A | A | G | G | A | A | A |
242 | C | T | T | C | T | T | T | C |
249 | G | C | C | G | C | C | C | C |
256 | A | G | G | A | G | G | G | A |
370 | T | C | C | T | C | C | T | T |
389 | C | T | T | C | T | T | C | C |
407 | G | A | A | G | A | A | G | G |
452 | T | C | C | C | C | T | C | C |
551 | T | C | C | T | T | T | T | T |
621 | G | T | T | T | G | G | G | G |
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Krotova, A.; Mazloum, A.; van Schalkwyk, A.; Prokhvatilova, L.; Gubenko, O.; Byadovskaya, O.; Chvala, I.; Sprygin, A. The Characterization and Differentiation of Recombinant Lumpy Skin Disease Isolates Using a Region within ORF134. Appl. Microbiol. 2023, 3, 35-44. https://doi.org/10.3390/applmicrobiol3010003
Krotova A, Mazloum A, van Schalkwyk A, Prokhvatilova L, Gubenko O, Byadovskaya O, Chvala I, Sprygin A. The Characterization and Differentiation of Recombinant Lumpy Skin Disease Isolates Using a Region within ORF134. Applied Microbiology. 2023; 3(1):35-44. https://doi.org/10.3390/applmicrobiol3010003
Chicago/Turabian StyleKrotova, Alena, Ali Mazloum, Antoinette van Schalkwyk, Larisa Prokhvatilova, Olesya Gubenko, Olga Byadovskaya, Ilya Chvala, and Alexander Sprygin. 2023. "The Characterization and Differentiation of Recombinant Lumpy Skin Disease Isolates Using a Region within ORF134" Applied Microbiology 3, no. 1: 35-44. https://doi.org/10.3390/applmicrobiol3010003