Proteogenomics Analysis Reveals Novel Micropeptides in Primary Human Immune Cells
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Bioinformatics Analysis
4.2. ORF Cloning and Confocal Microscopy
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Number of Putative Micropeptide Encoding Events | |
---|---|
Myeloid (CD1c+CD304−CD19−CD14−CD3−) Dendritic cells | 352 |
Plasmacytoid (CD1c−CD304+CD19−CD14−CD3−) Dendritic cells | 384 |
Classical monocyte (CD14+CD16−) cell proteome | 2513 |
Classical monocyte (CD14+CD16−) cell secretome | 727 |
Intermediate monocyte (CD14+CD16+) cell secretome | 291 |
Non-classical monocyte (CD14dimCD16+) cell secretome | 665 |
Primary macrophage extracellular vesicle (EV) | 390 |
Primary macrophage rest-secretome (RS) | 608 |
Total putative micropeptide encoding events | 5930 |
Non-redundant putative micropeptide encoding events | 2815 |
Very high confidence putative micropeptide encoding events | 185 |
Peptide | Findings from Kim et al. | Findings from the Subbannayya et al. |
---|---|---|
AGNIELFSK | Described as a novel coding region exon of C15orf52, expressed in Adult adrenal gland | Originated from lnc-CCDC9B-3:3, maps to 3′ UTR of CCDC9B, Expressed in classical monocyte secretome |
EAGAGAEAAAGSARPLGR | Described as a gene extension of NPLOC4, Expressed in B cells, Adult testis, Adult ovary, Fetal ovary | Originated from lnc-PDE6G-2:1, matches 5′ UTR of NPLOC4 gene, Expressed in classical monocyte proteome |
NDDIPEQDSLGLSNLQK | Described as a novel coding region of MKKS, Ubiquitous expression in adult tissues including immune cells (Monocytes, CD4+ T cells, B cells, CD8+ T cells | Originated from lnc-MKKS-4:1, Matches UTR region of MKKS gene, Expressed in classical monocyte proteome |
NSTLSEPGSGR | Described as a pseudogene (HDGFP1), Expressed in CD8+ T cells | Originated from lnc-OR13H1-3:1, Matches HDGFP pseudogene NST00000405943.3, Expressed in Primary macrophages resting secretome |
TQNNLESDYLAR | Described as a C-terminal protein extension of AAK1, Expressed in several regions, including Monocytes, CD4+ T cells, CD8+ T cells and B cells | Originated from lnc-AAK1-1:2, Matches intronic region of AAK1, Expressed in Classical monocyte proteome |
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Subbannayya, Y.; Bhatta, A.; Pinto, S.M.; Fitzgerald, K.A.; Kandasamy, R.K. Proteogenomics Analysis Reveals Novel Micropeptides in Primary Human Immune Cells. Immuno 2022, 2, 283-292. https://doi.org/10.3390/immuno2020018
Subbannayya Y, Bhatta A, Pinto SM, Fitzgerald KA, Kandasamy RK. Proteogenomics Analysis Reveals Novel Micropeptides in Primary Human Immune Cells. Immuno. 2022; 2(2):283-292. https://doi.org/10.3390/immuno2020018
Chicago/Turabian StyleSubbannayya, Yashwanth, Ankit Bhatta, Sneha M. Pinto, Katherine A. Fitzgerald, and Richard K. Kandasamy. 2022. "Proteogenomics Analysis Reveals Novel Micropeptides in Primary Human Immune Cells" Immuno 2, no. 2: 283-292. https://doi.org/10.3390/immuno2020018