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Comparison of the Hybrid Capture II Method with a PCR-Based Screening Method Using a Carboxyfluorescein-Labeled Primer for Detecting Human Papillomavirus in Cervicovaginal Liquid-Based Cytology

1
Divison of Pathophysiology, Okayama University Graduate School of Health Sciences, Okayama 700-8558, Japan
2
Department of Clinical Laboratory, Saijo Central Hospital, Saijo 793-0027, Japan
3
Department of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan
4
Department of Medical Technology, Ehime Prefectural University of Health Sciences, Iyo-gun 791-2101, Japan
*
Author to whom correspondence should be addressed.
J. Mol. Pathol. 2020, 1(1), 9-18; https://doi.org/10.3390/jmp1010003
Received: 8 July 2020 / Revised: 27 August 2020 / Accepted: 7 September 2020 / Published: 10 September 2020
Objective: Human papillomaviruses (HPVs) are DNA viruses, of which over 120 types have been identified. The main screening methods for HPV-DNA include the hybrid capture II (HC-II) and polymerase chain reaction (PCR) assays. Liquid-based cytology (LBC) is a high-quality technique developed to improve the diagnostic reliability of traditional Papanicolaou tests (Pap tests). However, relatively few studies have compared the efficacy of PCR and HC-II assays using cervicovaginal LBC specimens. In this study, we conducted a comparative analysis with results derived from the HC-II assay to assess whether a PCR-based assay using a novel carboxyfluorescein (FAM)-labeled primer could be applied to cervicovaginal LBC specimens. Methods and Results: We analyzed 59 specimens diagnosed as atypical squamous cells of undetermined significance (ASCUS) by Pap tests. After extracting DNA from cervicovaginal LBC specimens, we performed PCR using a FAM-labeled consensus primer, and then conducted fragment analysis to confirm the results. The value of the kappa statistic measuring the agreement between the PCR and HC-II results was 0.8557, or “almost perfect agreement.” Conclusion: Our novel HPV-PCR assay can be successfully applied to cervicovaginal LBC specimens for the detection of HPV subtypes. View Full-Text
Keywords: human papillomavirus; polymerase chain reaction; hybrid capture II assay; liquid-based cytology; cervical cancer human papillomavirus; polymerase chain reaction; hybrid capture II assay; liquid-based cytology; cervical cancer
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Saiki, Y.; Gion, Y.; Nishikori, A.; Norimatsu, Y.; Sato, Y. Comparison of the Hybrid Capture II Method with a PCR-Based Screening Method Using a Carboxyfluorescein-Labeled Primer for Detecting Human Papillomavirus in Cervicovaginal Liquid-Based Cytology. J. Mol. Pathol. 2020, 1, 9-18.

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