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Proceeding Paper

Spectroscopic Ellipsometry Detection of Prostate Cancer Bio-Marker PCA3 Using Specific Non-Labeled Aptamer: Comparison with Electrochemical Detection †

1
Material and Engineering Research Institute, City Campus, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, UK
2
Biomolecular Research Centre, City Campus, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, UK
3
Department of Chemistry, The Dainton Building, The University of Sheffield, Brook Hill, Sheffield S3 7HF, UK
*
Author to whom correspondence should be addressed.
Presented at the 1st International Electronic Conference on Chemical Sensors and Analytical Chemistry, 1–15 July 2021; Available online: https://csac2021.sciforum.net/.
Academic Editor: Maria Emília de Sousa
Chem. Proc. 2021, 5(1), 65; https://doi.org/10.3390/CSAC2021-10453
Published: 30 June 2021
The most common prostate cancer (PCa) diagnostics, which are based on detection of prostate-specific antigens (PSA) in blood, have specificity limitations often resulting in both false-positive and false-negative results; therefore, improvement in PCa diagnostics using more specific PCa biomarkers is of high importance. Studies have shown that the long noncoding RNA Prostate Cancer Antigen 3 (lncPCA3) that is over-expressed in the urine of prostate cancer patients is an ideal biomarker for non-invasive early diagnostics of PCa. Geno-sensors based on aptamer bioreceptors (aptasensors) offer cost- and time-effective, and precise diagnostic tools for detecting PCa biomarkers. In this study, we report on further developments of RNA-based aptasensors exploiting optical (spectroscopic ellipsometry) measurements in comparison with electrochemical (CV and IS) measurements published earlier. These sensors were made by immobilization of thiolated CG-3 RNA aptamers on the surface of gold. Instead of a redox-labelled aptamer used previously in electrochemical measurements, a non-labelled aptamer was used here in a combination with total internal reflection ellipsometry (TIRE) measurements. The results obtained by these two methods were compared. The method of TIRE is potentially highly sensitive and comparable in that respect with electrochemical methods capable of detection of PCA3 in sub-pM levels of concentration. The required selectivity is provided by the high affinity of PCA3-to-aptamer binding with KD in the 10−9 M range. The spectroscopic ellipsometry measurements provided additional information on the processes of PCA3 to aptamer binding. View Full-Text
Keywords: aptamer; mRNA; PCA3; biosensor; TIRE aptamer; mRNA; PCA3; biosensor; TIRE
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MDPI and ACS Style

Takita, S.; Nabok, A.; Smith, D.; Lishchuk, A. Spectroscopic Ellipsometry Detection of Prostate Cancer Bio-Marker PCA3 Using Specific Non-Labeled Aptamer: Comparison with Electrochemical Detection. Chem. Proc. 2021, 5, 65. https://doi.org/10.3390/CSAC2021-10453

AMA Style

Takita S, Nabok A, Smith D, Lishchuk A. Spectroscopic Ellipsometry Detection of Prostate Cancer Bio-Marker PCA3 Using Specific Non-Labeled Aptamer: Comparison with Electrochemical Detection. Chemistry Proceedings. 2021; 5(1):65. https://doi.org/10.3390/CSAC2021-10453

Chicago/Turabian Style

Takita, Sarra, Alexei Nabok, David Smith, and Anna Lishchuk. 2021. "Spectroscopic Ellipsometry Detection of Prostate Cancer Bio-Marker PCA3 Using Specific Non-Labeled Aptamer: Comparison with Electrochemical Detection" Chemistry Proceedings 5, no. 1: 65. https://doi.org/10.3390/CSAC2021-10453

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