Extremely Halophilic Biohydrogen Producing Microbial Communities from High-Salinity Soil and Salt Evaporation Pond
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. DNA Extraction and Amplicon Sequencing
2.3. Data Analysis
3. Results
3.1. Microbial Community Amplicon Sequencing
3.2. Alpha Diversity Analysis
3.3. Beta Diversity Analysis
3.4. OTU Heat Map
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample Name | Observed Species | Simpson | Shannon– Wiener | ACE | Chao1 | Good’s Coverage | PD Whole Tree |
---|---|---|---|---|---|---|---|
A1 | 109 | 0.189 | 0.777 | 117.204 | 118.231 | 1.000 | 13.401 |
A2 | 155 | 0.791 | 3.152 | 156.894 | 155.652 | 1.000 | 14.512 |
B | 68 | 0.228 | 0.904 | 96.987 | 99.667 | 1.000 | 6.243 |
OTU Number | Sequence Length (bp) | Closest Relative (NCBI Accession Number) | Similarity |
---|---|---|---|
OTU_128 | 448 | Halanaerobium fermentans R-9 (NR 024715.1) | 99.55% |
OTU_94 | 447 | Halanaerobacter lacunarum TB21 (KJ677978.1) | 97.45% |
OTU_40 | 449 | Halanaerobacter lacunarum TB21 (KJ677978.1) | 97.51% |
OTU_49 | 450 | Halanaerobacter lacunarum TB21 (KJ677978.1) | 99.03% |
OTU_158 | 438 | Halanaerobacter lacunarum TB21 (KJ677978.1) | 96.79% |
OTU_96 | 438 | Halanaerobacter lacunarum TB21 (KJ677978.1) | 96.80% |
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Taroepratjeka, D.A.H.; Imai, T.; Chairattanamanokorn, P.; Reungsang, A. Extremely Halophilic Biohydrogen Producing Microbial Communities from High-Salinity Soil and Salt Evaporation Pond. Fuels 2021, 2, 241-252. https://doi.org/10.3390/fuels2020014
Taroepratjeka DAH, Imai T, Chairattanamanokorn P, Reungsang A. Extremely Halophilic Biohydrogen Producing Microbial Communities from High-Salinity Soil and Salt Evaporation Pond. Fuels. 2021; 2(2):241-252. https://doi.org/10.3390/fuels2020014
Chicago/Turabian StyleTaroepratjeka, Dyah Asri Handayani, Tsuyoshi Imai, Prapaipid Chairattanamanokorn, and Alissara Reungsang. 2021. "Extremely Halophilic Biohydrogen Producing Microbial Communities from High-Salinity Soil and Salt Evaporation Pond" Fuels 2, no. 2: 241-252. https://doi.org/10.3390/fuels2020014