Next-Generation Sequencing Reveals Downregulation of the Wnt Signaling Pathway in Human Dysmature Cumulus Cells as a Hallmark for Evaluating Oocyte Quality

Akino, Ryosuke; Matsui, Daisuke; Kawahara-Miki, Ryouka; Amita, Mitsuyoshi; Tatsumi, Kuniko; Ishida, Eri; Kang, Woojin; Takada, Shuji; Miyado, Kenji; Sekizawa, Akihiko; Saito, Takakazu; Kono, Tomohiro; Saito, Hidekazu

doi:10.3390/reprodmed1030016

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Next-Generation Sequencing Reveals Downregulation of the Wnt Signaling Pathway in Human Dysmature Cumulus Cells as a Hallmark for Evaluating Oocyte Quality

^1,2,3,†,

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^1,*

Division of Reproductive Medicine, National Center for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan

Department of Obstetrics and Gynecology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8666, Japan

Department of Systems BioMedicine, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan

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Rose Ladies Clinic, 2-3-18 Todoroki, Setagaya, Tokyo 158-0082, Japan

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Nodai Genome Research Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan

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Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan

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Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan

Author to whom correspondence should be addressed.

^†

Authors contributed equally.

Reprod. Med. 2020, 1(3), 205-215; https://doi.org/10.3390/reprodmed1030016

Received: 14 November 2020 / Revised: 25 November 2020 / Accepted: 26 November 2020 / Published: 1 December 2020

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Abstract

Background: Dysmature cumulus cells are lower fertilization rates and abnormalities in embryonic development compared to maturation cumulus cells. Morphological evaluation of cumulus–oocyte complexes (COCs) considered the possibility that differences may also be found in gene expression. Purpose: To identify hallmarks for evaluating oocyte quality by investigating gene expression patterns in human cumulus cells surrounding oocytes. Methods: Cumulus cells were obtained from the cumulus–oocyte complex of infertile women treated with assisted reproductive technology. Based on maturity level, the cumulus cells were classified into two categories, i.e., dysmature cumulus cell (DCC) and maturation cumulus cell. DCCs were subjected to gene expression analysis using next-generation sequencing and compared with COCs that are in the process of maturation as controls. Results: The expression levels of genes involved in the Wnt signal/β-catenin pathway were significantly reduced in DCCs compared with those in control cells. Moreover, the expression levels of genes involved in multiple pathways associated with apoptosis were also significantly reduced compared with those in control cells. Conclusions: DCCs showed significant decreases in apoptosis- and Wnt/β-catenin signaling-associated gene expression. DCCs could be classified by morphological evaluation, and the method described in this study may be useful as an oocyte quality estimation tool. View Full-Text

Keywords: cumulus cells; female infertility; next-generation sequencing; oocyte quality; Wnt signaling pathway

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Akino, R.; Matsui, D.; Kawahara-Miki, R.; Amita, M.; Tatsumi, K.; Ishida, E.; Kang, W.; Takada, S.; Miyado, K.; Sekizawa, A.; Saito, T.; Kono, T.; Saito, H. Next-Generation Sequencing Reveals Downregulation of the Wnt Signaling Pathway in Human Dysmature Cumulus Cells as a Hallmark for Evaluating Oocyte Quality. Reprod. Med. 2020, 1, 205-215. https://doi.org/10.3390/reprodmed1030016

AMA Style

Akino R, Matsui D, Kawahara-Miki R, Amita M, Tatsumi K, Ishida E, Kang W, Takada S, Miyado K, Sekizawa A, Saito T, Kono T, Saito H. Next-Generation Sequencing Reveals Downregulation of the Wnt Signaling Pathway in Human Dysmature Cumulus Cells as a Hallmark for Evaluating Oocyte Quality. Reproductive Medicine. 2020; 1(3):205-215. https://doi.org/10.3390/reprodmed1030016

Chicago/Turabian Style

Akino, Ryosuke, Daisuke Matsui, Ryouka Kawahara-Miki, Mitsuyoshi Amita, Kuniko Tatsumi, Eri Ishida, Woojin Kang, Shuji Takada, Kenji Miyado, Akihiko Sekizawa, Takakazu Saito, Tomohiro Kono, and Hidekazu Saito. 2020. "Next-Generation Sequencing Reveals Downregulation of the Wnt Signaling Pathway in Human Dysmature Cumulus Cells as a Hallmark for Evaluating Oocyte Quality" Reproductive Medicine 1, no. 3: 205-215. https://doi.org/10.3390/reprodmed1030016

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Next-Generation Sequencing Reveals Downregulation of the Wnt Signaling Pathway in Human Dysmature Cumulus Cells as a Hallmark for Evaluating Oocyte Quality

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