Next Article in Journal / Special Issue
On the Importance of the Thiazole Nitrogen in Epothilones: Semisynthesis and Microtubule-Binding Affinity of Deaza-Epothilone C
Previous Article in Journal / Special Issue
Giant Polymer Compartments for Confined Reactions
Open AccessArticle

Combining the Sensitivity of LAMP and Simplicity of Primer Extension via a DNA-Modified Nucleotide

Department of Chemistry & Konstanz Research School Chemical Biology, University of Konstanz, 78457 Konstanz, Germany
*
Author to whom correspondence should be addressed.
Chemistry 2020, 2(2), 490-498; https://doi.org/10.3390/chemistry2020029
Received: 16 April 2020 / Revised: 11 May 2020 / Accepted: 11 May 2020 / Published: 14 May 2020
LAMP is an approach for isothermal nucleic acids diagnostics with increasing importance but suffers from the need of tedious systems design and optimization for every new target. Here, we describe an approach for its simplification based on a single nucleoside-5′-O-triphosphate (dNTP) that is covalently modified with a DNA strand. We found that the DNA-modified dNTP is a substrate for DNA polymerases in versatile primer extension reactions despite its size and that the incorporated DNA indeed serves as a target for selective LAMP analysis. View Full-Text
Keywords: modified nucleotide; DNA polymerase; LAMP; primer extension modified nucleotide; DNA polymerase; LAMP; primer extension
Show Figures

Figure 1

MDPI and ACS Style

Welter, M.; Marx, A. Combining the Sensitivity of LAMP and Simplicity of Primer Extension via a DNA-Modified Nucleotide. Chemistry 2020, 2, 490-498.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Back to TopTop