4.2. Pre and Post-Grazing Heights and Grazing Intervals
The highest number of grazing cycles was observed with 90% IL treatments (
Table 3) due to the lower grazing intervals (
Table 4). During the end of the spring and summer, characterized by good growth conditions, the mean grazing intervals were 27 days and 33 days for 90% and 95% LI treatments, respectively. For the winter/beginning of the spring period, marked by less favorable growth conditions, the mean grazing intervals were 93 days and 118 days, respectively, for treatments of 90% and 95% LI (
Table 4).
On average, the number of grazing cycles in the end of the spring and summer was 3.5 times higher than the winter/beginning of the spring period. This shows the great seasonality of this grass throughout the seasons, indicating that management goals that fix dates can hinder the management of this fodder plant. In another study with Guinea grass, Barbosa et al. [
7] reported grazing intervals during spring and summer of 29 days and 32 days for treatments of 90% and 95% LI, respectively; the number of grazing cycles was 3.6 times higher in the summer in relation to winter, consistent with results obtained in this experiment.
The highest number of grazing cycles was observed at the end of the spring and summer, a season with higher temperature (
Figure 1), rainfall (
Figure 2) and solar radiation, and also when nitrogen fertilization took place (
Table 1). Therefore, favorable climatic conditions were observed in the high growth seasons, reducing increasing grazing recovery time after grazing, which resulted in reduced intervals and increased grazing cycles (
Table 3 and
Table 4).
Introduction of animals onto the pastures was determined by the proportion of incident light that was intercepted by the canopy during regrowth. It was established that grazing would be initiated when there was 90% and 95% LI, with the goals for post-grazing heights of 30 cm and 50 cm. The mean post-grazing height was very consistent with each treatment during the whole experimental period (
Figure 3 and
Figure 4). Regardless of the level of LI during the pre-grazing period, it was possible to maintain the post-grazing height of 50 cm throughout the experimental period. Regarding the post-grazing height of 30 cm, there was a slightly greater variation, mainly with 95% LI, but this did not exceed 5 cm.
As observed by some authors [
18], there is a positive relationship between light trapping during regrowth and height of the pre-grazing forage canopy in tropical grasses. In this experiment, the height of the pre-grazing pasture was homogeneous, with values close to 65 cm and 75 cm for the 90% and 95% LI management, respectively (
Figure 4). These results are close to those reported by Barbosa et al. [
7], who obtained values of pre-grazing height around 60 cm and 70 cm for treatments of 90% and 95% LI, respectively, and Difante et al. [
16], who reported values close to 70 cm for the 95/30 treatment in Guinea grass pastures. As observed by some authors [
18], there is a positive relationship between light trapping during regrowth and height of the pre-grazing forage canopy in tropical grasses.
The highest values of pre-grazing forage mass were, in general, verified for the target of 95% of LI and for the post-grazing height of 30 cm in the end of the spring, summer and fall periods year (
Table 5). For winter/beginning of the spring, grass management at post-grazing height 50 cm produced a higher forage mass, with the 90/50 treatment preducing the highest value. Barbosa et al. [
7], in an evaluation of Guinea grass, did not demonstrate differences between post-grazing heights of 25 cm and 50 cm in winter/beginning of the spring period for pre-grazing forage mass.
4.3. Forage Mass and Pre-Grazing Morphological Composition
The pastures managed with 95% LI, in addition to producing a greater mass of forage, showed, with respect to the morphological composition, little contribution of stem and dead material and no difference in relation to those management with 90% of LI (
Figure 5). This is an indication that the 95% LI pre-grazing target may be appropriate for the management of this species, as it promoted a higher amount of forage mass, with a high percentage of leaves and a low percentage of stalk and dead material. Pre-grazing management using LI over 100%, as in the case of Barbosa et al. [
7] for the same forage plant, resulted in a higher value of forage mass, but with reduced nutritional value caused by higher proportion of stem and dead material.
The of post-grazing height of 30 cm, in general, was higher value of forage mass during the most productive periods, with the seasons of the end of the spring, summer and fall, responsible for an average increment greater than 15 kg DM/ha.day, at the post-grazing height of 30 cm in relation to that of 50 cm (
Table 5). However, the number of grazing cycles was lower with the 30 cm target (
Table 3), due to the lower post-grazing height that led to a lower regrowth and provided a longer grazing interval. This means that the forage mass may higher, but there were fewer grazing cycles.
Despite this, the evaluation of the morphological composition of the forage at the lowest post-grazing height (30 cm) indicated a lower contribution of stem and dead material (
Figure 5). Difante et al. [
16], in an experiment with two post-grazing heights of 25 cm and 50 cm associated with 95% LI pre-grazing management, did not report differences between the post-grazing heights in Guinea grass. In the present experiment, it was only during the winter/beginning of the spring period that the post-grazing height of 50 cm resulted in pre-grazing forage mass values that were higher than the post-grazing height of 30 cm, probably due to the longer grazing interval during this period increasing the contribution of dead material to the forage mass.
Thus, as can be seen in
Table 5, the association between the highest grazing interval and the lowest post-grazing height represented by the 95/30 treatment, presented basically the highest forage mass values in relation to the other treatments. Considering the values of the morphological components in this treatment, there seems to be a good relationship between high forage mass and nutritive value (
Table 7 and
Table 8). Consequence of the high percentage of leaf blades and low contribution of stalk and dead material (
Figure 5).
Of all the seasons of the year, the higher value of pre-grazing pasture mass was recorded in end of the spring and summer (
Table 5), which may be a consequence of the higher growth rate observed during these periods, i.e., higher of leaf elongation, appearance of leaves and stem [
7].
The highest proportion of dead material occurred in the winter/beginning of the spring period (38.7%) (
Table 6), a consequence of the higher senescence rates recorded at that time of the year. A similar result was observed by Barbosa et al. [
19] that registered values of 40% of dead material in the winter/spring period.
Content of DM, NDF, ADF, LIG, NDIN and ADIN had values higher than 2.62, 1.67, 3.14, 1.39, 2.51 and 1.81 g/kg, respectively, in relation to the handling with 90% LI (
Table 7 and
Table 8); this superiority, is possibly related to the maturity of leaves and stems of the plants managed with the longest interval between grazing (95 LI). According to Zanine et al. [
20], DM, NDF, ADF, hemicellulose and lignin content were higher in stems, senescent leaves and expanded (mature) leaves in Guinea grass, and higher CP levels and lower levels of NDF and ADF occurred in growing leaves and recently expanded leaves. The authors concluded that in terms of nutritional value, there was a decreasing hierarchy as follows: growing leaf, expanded leaf, whole plant and stem; this indicated that the longer the interval between grazing, the greater the nutritional losses from the pasture.
In general, the treatments did not present differences between the 90% and 95% LI managed, in terms of the proportion of leaf blades in the pre-grazing forage mass (
Figure 5); therefore, the best nutritional value of pasture managed with 90% IL, not be related only to pasture structure. Possibly, the best nutritional value of this treatment would also be related to the proportion of growing leaves, recently expanded leaves and new stem, with lower maturity, inherent in the management of 90% LI, since this forage was harvested earlier than the management of 95% LI. Smaller plants have higher levels of soluble carbohydrates [
8], justifying also the superiority in the nutritional value of 90% LI management.
According to Machado o et al. [
21], when considering the development stages of plants, it has been demonstrated that, as they grow, they decrease in density and the proportion of leaves and increase the proportion of the stem. This means that there is an increase in the content of structural compounds, such as cellulose, hemicellulose and lignin, and, in parallel, a decrease in the cellular content. These changes lead to reduced intake and digestibility and, consequently, reduced supply of energy, as well as that of other nutrients.
The result of this management with less grazing frequency (90% LI) is a forage harvest with higher nutritional value, which in the present study was marked by the highest TC, NFC, CP and in vitro digestible dry matter (IVDDM) (
Table 7 and
Table 8). Results that agree with Valente et al. [
22], who investigated the nutritional value, digestibility and degradability of Guinea grass pastures with different grazing frequencies with sheep. The authors concluded that the increase in the interval between grazing decreases the nutritional characteristics of pasture, especially when this value exceeds 95% LI.
In the present study, it is worth noting that the best nutritional values were demonstrated in the management of grazing frequency with 90% LI. However, nutritional values in the management of 95% LI were satisfactory, and it represents the greater productivity of Forage mass (
Table 5), with a good percentage of leaf blade (
Table 6,
Figure 5) in the most productive periods, such as end of the spring and summer.
The CP levels below 7% are limiting for animal production, as they are associated with lower voluntary intake, reduced digestibility and negative nitrogen balance [
22]. It is noteworthy that Guinea grass would satisfactorily meet ruminant protein crude requirements independent of the seasons, and the management of 90 or 95% LI.
The increase of approximately 2 g/kg of Guinea grass IVDDM in the management of 90% of LI in the pasture is associated with the maintenance of a slightly lower pasture, favoring constant regrowth, with maintenance of young leaves and tillers; in higher pastures, there is maintenance of leaves in phase of greater maturation. This is shown by the slightly higher increase of NDF, ADF, lignin, NDIN and ADIN in the Guinea grass forage mass when managed at a greater length (95% LI) (
Table 7 and
Table 8).
Thus, the management of Guinea grass pastures under rotational grazing should involve introducing the animals when 95% of the incident light is being intercepted, that is when grass is at a height of 75 cm, and remove stock when grass is at a height of 30 cm. This would be this case if the main intention of the producer was to improve the animal gain per unit area, that is, more forage with a slightly lower nutritional value. However, if the desire was to improve the gain per animal, management could be made more flexible by introducing the animals a little earlier, with 90% LI or grass at a height of 65 cm. This would reduce the amount of forage by reducing the rest period and, as a consequence, provide earlier forage with better nutritional value and without damage to pasture productivity.