The Effects of General Anaesthesia and Light on Behavioural Rhythms and GABAA Receptor Subunit Expression in the Mouse SCN
Abstract
:1. Introduction
2. Results
2.1. Effects of GA and Light on Locomotor Rhythms of C57 Mice
2.2. GABAAR Subunit Expression in the SCN under LD Conditions
2.3. The Effects of GA and Light on GABAAR Subunit Expression in the SCN at Times of Maximal Behavioural Phase Shifts (CT9–CT13)
2.4. The Effects of GA and Light on GABAAR Subunit Expression in the SCN at Times of Minimal Behavioural Phase Shifts (CT3–CT7)
3. Discussion
4. Materials and Methods
4.1. Behavioural Experiments
4.2. Behavioural Analysis
4.3. Immunohistochemistry Experiments
4.4. Immunohistochemistry
4.5. Imaging and Analysis
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Light | GA+Light | GA in DD (Ludin et al., 2021) | |||
---|---|---|---|---|---|
CT (range) | n | Shift (SE) (h) | n | Shift (SE) (h) | Shift (SE) (h) |
2 (0.4–3.8) | 11 | 0.30 (0.13) | 7 | 0.08 (0.16) | 0.17 (0.14) |
6 (4.1–7.9) | 11 | 0.33 (0.16) | 10 | −0.16 (0.21) | −0.11 (0.38) |
10 (8.0-–2.0) | 8 | 0.53 (0.17) | 9 | −0.82 (0.52) | −0.61 (0.31) |
14 (14.2–15.9) | 6 | −3.22 (0.29) | 6 | −1.87 (0.26) | −0.77 (0.18) |
18 (16–19.6) | 15 | −3.27 (0.21) | 14 | −0.45 (0.20) | −0.25 (0.11) |
22 (20.2–24) | 7 | −1.08 (0.69) | 14 | 0.25 (0.12) | 0.23 (0.14) |
ZT0 | ZT6 | ZT12 | ZT18 | |||||
---|---|---|---|---|---|---|---|---|
n | Mean (SE) | n | Mean (SE) | n | Mean (SE) | n | Mean (SE) | |
Alpha 1 | 5 | 0.462 (0.015) | 6 | 0.542 (0.025) 1,2 | 5 | 0.411 (0.042) 1 | 6 | 0.361 (0.029) 2 |
Beta 3 | 2 | 0.728 (0.057) 3 | 4 | 0.400 (0.066) 3 | 4 | 0.456 (0.057) | 5 | 0.568 (0.065) |
Gamma 2 | 3 | 0.633 (0.019) | 4 | 0.426 (0.046) | 6 | 0.412 (0.066) 4 | 6 | 0.609 (0.025) 4 |
Treatment | Light | GA + Light | GA in DD | |||
---|---|---|---|---|---|---|
n | Mean (SE) | n | Mean (SE) | n | Mean (SE) | |
Alpha 1 | 5 | 0.440 (0.114) | 5 | 0.409 (0.067) | 4 | 0.449 (0.059) |
Beta 3 | 5 | 0.526 (0.027) 1 | 5 | 0.284 (0.052) 1,2 | 4 | 0.486 (0.061) 2 |
Gamma 2 | 5 | 0.423 (0.083) 3 | 5 | 0.592 (0.051) | 4 | 0.698 (0.009) 3 |
Treatment | Light | GA + Light | GA in DD | |||
---|---|---|---|---|---|---|
n | Mean (SE) | n | Mean (SE) | n | Mean (SE) | |
Alpha 1 | 5 | 0.056 (0.024) 1 | 5 | 0.113 (0.031) | 3 | 0.261 (0.073) 1 |
Beta 3 | 5 | 0.486 (0.039) 2 | 5 | 0.445 (0.045) | 3 | 0.271 (0.034) 2 |
Gamma 2 | 5 | 0.313 (0.051) | 5 | 0.371 (0.065) | 3 | 0.467 (0.046) |
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Chong, J.; Cheeseman, J.F.; Pawley, M.D.M.; Kwakowsky, A.; Warman, G.R. The Effects of General Anaesthesia and Light on Behavioural Rhythms and GABAA Receptor Subunit Expression in the Mouse SCN. Clocks & Sleep 2021, 3, 482-494. https://doi.org/10.3390/clockssleep3030034
Chong J, Cheeseman JF, Pawley MDM, Kwakowsky A, Warman GR. The Effects of General Anaesthesia and Light on Behavioural Rhythms and GABAA Receptor Subunit Expression in the Mouse SCN. Clocks & Sleep. 2021; 3(3):482-494. https://doi.org/10.3390/clockssleep3030034
Chicago/Turabian StyleChong, Janelle, James Frederick Cheeseman, Matthew D. M. Pawley, Andrea Kwakowsky, and Guy R. Warman. 2021. "The Effects of General Anaesthesia and Light on Behavioural Rhythms and GABAA Receptor Subunit Expression in the Mouse SCN" Clocks & Sleep 3, no. 3: 482-494. https://doi.org/10.3390/clockssleep3030034