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Article

Association of Interleukins Genes Polymorphisms with Multi-Drug Resistant Tuberculosis in Ukrainian Population

by
Dmytro O. Butov
1,*,
Mykhaylo M. Kuzhko
2,
Natalia I. Makeeva
1,
Tetyana S. Butova
1,
Hanna L. Stepanenko
1 and
Andrii B. Dudnyk
3
1
Kharkiv National Medical University, Kharkiv, Ukraine
2
National Institute of Phthisiology and Pulmonology Named after F. G. Yanovskyi NAMS of Ukraine, Kiev, Ukraine
3
National Pirogov Memorial Medical University, Vinnytsia, Ukraine
*
Author to whom correspondence should be addressed.
Adv. Respir. Med. 2016, 84(3), 168-173; https://doi.org/10.5603/PiAP.2016.0019
Submission received: 24 November 2015 / Revised: 28 May 2016 / Accepted: 28 May 2016 / Published: 28 May 2016

Abstract

Introduction: Multi-drug resistant tuberculosis (MDR TB) is a significant health problem in some parts of the world. Three major cytokines involved in TB immunopathogenesis include IL-2, IL-4 and IL-10. The susceptibility to MDR TB may be genetically determined. The aim of the study was to assess the association of IL-2, IL-4, IL-10 gene polymorphisms with multi-drug resistant tuberculosis (MDR TB) in Ukrainian population. Material and methods: We observed 140 patients suffering from infiltrative pulmonary tuberculosis (PT) and 30 apparently healthy subjects. The patients were assigned to two groups whether they suffer or do not suffer from pulmonary MDR TB. Interleukin gene (IL) polymorphisms, particularly T330G polymorphism in the IL-2 gene, C589T polymorphism in the IL-4 gene and G1082A polymorphism in the IL-10 gene were studied through polymerase chain reaction. Circulating levels of IL-2, IL-4 and IL-10 in venous blood were estimated using ELISA. Results: Prior to treatment, patients with PT showed significant increase of IL-2 levels and decrease of IL-4 and IL-10 levels compared to apparently healthy subjects. Circulating IL-4 and IL-10 levels were significantly decreased whilst serum IL-2 level was significantly increased in patients with MDR TB compared to non-MDR TB. Low IL-4 and IL-10 secretion and considerable IL-2 alterations were shown to be significantly associated with mutations of homozygous and heterozygous genotypes affecting C589T polymorphism in the IL-4 gene, G1082A polymorphism in the IL-10 gene and T330G polymorphism in the IL-2 gene in patients with PT. Conclusions: Heterozygous genotype and mutations homozygous genotypes gene in polymorphisms determining specified cytokines’ production is a PT risk factor and may lead to disease progression into chronic phase. Heterozygous genotype of aforementioned cytokine genetic polymorphisms was significantly the most frequent in patients with MDR TB.
Keywords: multi-drug resistant tuberculosis; IL-2; IL-4; IL-10; gene polymorphisms multi-drug resistant tuberculosis; IL-2; IL-4; IL-10; gene polymorphisms

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MDPI and ACS Style

Butov, D.O.; Kuzhko, M.M.; Makeeva, N.I.; Butova, T.S.; Stepanenko, H.L.; Dudnyk, A.B. Association of Interleukins Genes Polymorphisms with Multi-Drug Resistant Tuberculosis in Ukrainian Population. Adv. Respir. Med. 2016, 84, 168-173. https://doi.org/10.5603/PiAP.2016.0019

AMA Style

Butov DO, Kuzhko MM, Makeeva NI, Butova TS, Stepanenko HL, Dudnyk AB. Association of Interleukins Genes Polymorphisms with Multi-Drug Resistant Tuberculosis in Ukrainian Population. Advances in Respiratory Medicine. 2016; 84(3):168-173. https://doi.org/10.5603/PiAP.2016.0019

Chicago/Turabian Style

Butov, Dmytro O., Mykhaylo M. Kuzhko, Natalia I. Makeeva, Tetyana S. Butova, Hanna L. Stepanenko, and Andrii B. Dudnyk. 2016. "Association of Interleukins Genes Polymorphisms with Multi-Drug Resistant Tuberculosis in Ukrainian Population" Advances in Respiratory Medicine 84, no. 3: 168-173. https://doi.org/10.5603/PiAP.2016.0019

APA Style

Butov, D. O., Kuzhko, M. M., Makeeva, N. I., Butova, T. S., Stepanenko, H. L., & Dudnyk, A. B. (2016). Association of Interleukins Genes Polymorphisms with Multi-Drug Resistant Tuberculosis in Ukrainian Population. Advances in Respiratory Medicine, 84(3), 168-173. https://doi.org/10.5603/PiAP.2016.0019

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