Next Article in Journal
Identification of Mycobacteriaceae Species Based on the Hsp-65 Gene Polymorphism Analysis by PCR–RFLP
Previous Article in Journal
Pulmonary Mycobacteriosis—The Diagnostic Challenge. The Authors’ Experience
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
ARM
Volume 74
Issue 1
10.5603/ARM.28078
arm-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Advances in Respiratory Medicine is published by MDPI from Volume 90 Issue 4 (2022). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Via Medica.
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

The Mycolic Acids Analysis with Hplc Technique in Drug Susceptibility Testing of Mycobacterium Tuberculosis Strains †

by
Renata Walkiewicz
*,
Hanna Grubek-Jaworska
and
Ryszarda Chazan
Katedra i Klinika Chorób Wewnętrznych, Pneumonologii i Alergologii AM w Warszawie, Warsaw, Poland
*
Author to whom correspondence should be addressed.
†
Pracę wykonano w ramach realizacji projektu badawczego 2P05D00128 finansowanego przez Ministerstwo Nauki i Informatyzacji.
Adv. Respir. Med. 2006, 74(1), 89-94; https://doi.org/10.5603/ARM.28078
Submission received: 1 March 2006 / Revised: 1 March 2006 / Accepted: 1 March 2006 / Published: 1 March 2006

Abstract

The aim of this study was to evaluate the utility of the quantitative analysis of mycolic acids by HPLC technique in drug susceptibility testing of the M. tuberculosis isolates to the first-line antituberculous drugs: isoniazid and rifampicin. Drug susceptibility of the 30 clinical M.tbc isolates was examined by the mycolic acids analysis with HPLC technique and results were compared to the proportion method on solid L-J medium and liquid medium in MGIT system. In HPLC method drug susceptibility of M. tuberculosis strains was described by TAMA index defined as the ratio of thetotal area under mycolic acids peaks (TAMA) from cultures with drug to the TAMA of control. At critical concentrations of drugs, TAMA indexes of resistant strains were >0.5, and TAMA indexes of susceptible strains were <0.05. The average error of the TAMA analysis was ±9.5% The quantitative analysis of mycolic acids by HPLC gives results compatible with standard proportion method and is a reliable method for determination of drug susceptibility of M. tuberculosis.
Keywords: Mycolic acids; high pressure liquid chromatography HPLC; M. tuberculosis; drug resistance Mycolic acids; high pressure liquid chromatography HPLC; M. tuberculosis; drug resistance

Share and Cite

MDPI and ACS Style

Walkiewicz, R.; Grubek-Jaworska, H.; Chazan, R. The Mycolic Acids Analysis with Hplc Technique in Drug Susceptibility Testing of Mycobacterium Tuberculosis Strains. Adv. Respir. Med. 2006, 74, 89-94. https://doi.org/10.5603/ARM.28078

AMA Style

Walkiewicz R, Grubek-Jaworska H, Chazan R. The Mycolic Acids Analysis with Hplc Technique in Drug Susceptibility Testing of Mycobacterium Tuberculosis Strains. Advances in Respiratory Medicine. 2006; 74(1):89-94. https://doi.org/10.5603/ARM.28078

Chicago/Turabian Style

Walkiewicz, Renata, Hanna Grubek-Jaworska, and Ryszarda Chazan. 2006. "The Mycolic Acids Analysis with Hplc Technique in Drug Susceptibility Testing of Mycobacterium Tuberculosis Strains" Advances in Respiratory Medicine 74, no. 1: 89-94. https://doi.org/10.5603/ARM.28078

APA Style

Walkiewicz, R., Grubek-Jaworska, H., & Chazan, R. (2006). The Mycolic Acids Analysis with Hplc Technique in Drug Susceptibility Testing of Mycobacterium Tuberculosis Strains. Advances in Respiratory Medicine, 74(1), 89-94. https://doi.org/10.5603/ARM.28078

Article Metrics

Back to TopTop