Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds
Abstract
:1. Introduction
2. Results and Discussion
2.1. AFM Analysis of the Wrinkle-CS Films in Air and PBS
2.2. 3T3 Cell Culture on the Wrinkle-CS Film
2.3. 3T3 Biocompatibility of the Wrinkle-CS Film
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Instrumentation
4.3. Preparation of the CS Film
4.4. Surface Wrinkling of Films
4.5. AFM and Force Curve Measurement
4.6. In Vitro Cell Culture
4.7. Animals
4.8. In Vivo Evaluation
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
CS | Chitosan |
HRP | Horseradish peroxidase |
PBS | Phosphate buffered saline |
AFM | Atomic force microscopy |
FE | Ferulic acid |
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Izawa, H.; Okuda, N.; Yonemura, T.; Kuroda, K.; Ochi, K.; Ifuku, S.; Morimoto, M.; Saimoto, H.; Noda, M.; Azuma, K.; et al. Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds. Colloids Interfaces 2018, 2, 15. https://doi.org/10.3390/colloids2020015
Izawa H, Okuda N, Yonemura T, Kuroda K, Ochi K, Ifuku S, Morimoto M, Saimoto H, Noda M, Azuma K, et al. Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds. Colloids and Interfaces. 2018; 2(2):15. https://doi.org/10.3390/colloids2020015
Chicago/Turabian StyleIzawa, Hironori, Noriko Okuda, Tomoe Yonemura, Kohei Kuroda, Kosuke Ochi, Shinsuke Ifuku, Minoru Morimoto, Hiroyuki Saimoto, Mayuko Noda, Kazuo Azuma, and et al. 2018. "Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds" Colloids and Interfaces 2, no. 2: 15. https://doi.org/10.3390/colloids2020015