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Colloids Interfaces 2018, 2(2), 15;

Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds

Graduate School of Engineering, Tottori University, 4-101 Koyama-Minami, Tottori 680-8550, Japan
Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-Minami, Tottori 680-8550, Japan
Division of Instrumental Analysis, Research Center for Bioscience and Technology, Tottori University, Tottori 680-8550, Japan
Authors to whom correspondence should be addressed.
Received: 2 March 2018 / Revised: 21 March 2018 / Accepted: 23 March 2018 / Published: 26 March 2018
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Microscopic surface architectures that can be easily manufactured have been in demand as mechano-structural cues for tissue engineering. Microscopic surface reliefs synthesized by wrinkling were expected as cell culture scaffolds for cell proliferation, control of cellular alignment and differentiation, and spheroid generation. We previously developed bio-based wrinkled films prepared via lignification-mimetic reactions and drying. Although these films are expected as a candidate for cell culture scaffolds, stability and morphology of the wrinkled surfaces in aqueous buffer solutions were not explored. Here, we investigate the surface morphologies of the wrinkled films in phosphate-buffered saline, and their application to 3T3 cell culture. The wrinkled film prepared with the immersion treatment at 40 °C maintained its wrinkled structure in phosphate-buffered saline even after five days, although the wrinkles were broadened by hydration of the skin layer. Interestingly, higher cell numbers were observed in the 3T3 cell culture using the wrinkled film than using flat film with the same surface composition. In addition, the high biocompatibility of the wrinkled film was confirmed by in vivo experiments. These results strongly encourage application of the wrinkled film as a mechano-structural cue. Studies of the advanced applications for the wrinkled films are now in progress. View Full-Text
Keywords: chitosan; surface wrinkling; skin layer; horseradish peroxidase; cell culture substrate; 3T3 cell chitosan; surface wrinkling; skin layer; horseradish peroxidase; cell culture substrate; 3T3 cell

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Izawa, H.; Okuda, N.; Yonemura, T.; Kuroda, K.; Ochi, K.; Ifuku, S.; Morimoto, M.; Saimoto, H.; Noda, M.; Azuma, K.; Okamoto, Y.; Ito, N. Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds. Colloids Interfaces 2018, 2, 15.

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Colloids Interfaces EISSN 2504-5377 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
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