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Proceeding Paper

Novel Strategy for the Formulation of Poorly Water-Soluble Drugs: Nystatin Microencapsulation †

Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain
Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, 08028 Barcelona, Spain
Pharmacy Department, Torrecárdenas Hospital, 04009 Almería, Spain
Biomedical Research Institute ibs.Granada, 18014 Granada, Spain
Author to whom correspondence should be addressed.
Presented at the 1st International ElectronicConference on Pharmaceutics, 1–15 December2020; Available online:
Proceedings 2021, 78(1), 41;
Published: 4 December 2020
(This article belongs to the Proceedings of The 1st International Electronic Conference on Pharmaceutics)

Abstract: Background

In recent years, a growing concern about resistance to anti-infective agents has emerged1. One of the most common microbial agents is Candida albicans. Under certain conditions, C. albicans can cause infections of skin and mucosal tissues. Nystatin (Nys) is a broad-spectrum antifungal, which is indicated for the treatment of mucosal infections caused by Candida ssp such as patients under radiological treatment. Nys is a photosensitive drug and very poorly soluble in aqueous media. Therefore, microencapsulation can be the solution for its limiting factors2–3. Purpose: The aim of this work was to design, develop and characterize two types of microparticles as appropriate nystatin delivery systems for topical use: alginate microparticles (AM) and chitosan coated alginate microparticles (CCM). Methods: The formulation of the microparticles was based on the emulsification/internal gelation methodology with modification4. First, a water in oil W/O emulsion was created. Sodium alginate aqueous solution, CaCO3 and Nys were the ingredients of the internal phase, and vegetable oil the external phase. The resulting microparticles were characterized in terms of particle size, percentage yield (PY), loading capacity (LD), encapsulation efficiency (EE) and mucoadhesion ability. Results and Discussion: Microparticles ranged from 51.21 μm for AM to 57.20 μm for CCM. The PY values were 83.26% and for 79.67% AM and CCM, respectively. The LD values for the inside/surface were 6.78%/0.40% for AM and 4.87%/0.91% for CCM. The values of EE for inside and surface were 81.12%/12.07% for AM and 85.08%/9.19% for CCM. CCM was the system that exhibited the best mucoadhesive properties. Conclusions: The ability of these systems to adhere to mucous membranes has great appeal for the treatment of localized infections. Thus, these microparticulate systems could be proposed as a suitable vehicle for this kind of mucosal infections, being an alternative therapy.

Supplementary Materials

The following are available online at

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.


  1. Fernández-Campos, F.; Clares-Naveros, B.; López-Serrano, O.; Alonso-Merino, C.; Calpena-Campmany, A.C. Evaluation of Novel Nystatin Nanoemulsion for Skin Candidosis Infections. Mycoses 2013, 56, 70–81. [Google Scholar] [CrossRef] [PubMed]
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MDPI and ACS Style

Pérez-González, N.; Martín-Villena, M.J.; Calpena-Campmany, A.C.; Morales-Molina, J.A.; Clares-Naveros, B. Novel Strategy for the Formulation of Poorly Water-Soluble Drugs: Nystatin Microencapsulation. Proceedings 2021, 78, 41.

AMA Style

Pérez-González N, Martín-Villena MJ, Calpena-Campmany AC, Morales-Molina JA, Clares-Naveros B. Novel Strategy for the Formulation of Poorly Water-Soluble Drugs: Nystatin Microencapsulation. Proceedings. 2021; 78(1):41.

Chicago/Turabian Style

Pérez-González, Noelia, María J. Martín-Villena, Ana C. Calpena-Campmany, José A. Morales-Molina, and Beatriz Clares-Naveros. 2021. "Novel Strategy for the Formulation of Poorly Water-Soluble Drugs: Nystatin Microencapsulation" Proceedings 78, no. 1: 41.

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