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Proceeding Paper

2D Nanomaterial, Ti3C2 MXene-Based Sensor to Guide Lung Cancer Therapy and Management †

by
Mahek Sadiq
1,
Lizhi Pang
2,
Michael Johnson
3,
Venkatachalem Sathish
2 and
Danling Wang
1,3,4,*
1
Biomedical Engineering Program, North Dakota State University, Fargo, ND 58108, USA
2
Department of Pharmaceutical Science, North Dakota State University, Fargo, ND 58108, USA
3
Materials and Nanotechnology Program, North Dakota State University, Fargo, ND 58108, USA
4
Department of Electrical and Computer Engineering, North Dakota State University, Fargo, ND 58108, USA
*
Author to whom correspondence should be addressed.
†
Presented at the 1st International Electronic Conference on Biosensors, 2–17 November 2020; Available online: https://iecb2020.sciforum.net/.
Proceedings 2020, 60(1), 29; https://doi.org/10.3390/IECB2020-07055
Published: 2 November 2020
(This article belongs to the Proceedings of The 1st International Electronic Conference on Biosensors)
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Abstract

:
Major advances in cancer control can be greatly aided by early diagnosis and effective treatment in its pre-invasive state. Lung cancer (small cell and non-small cell) is a leading cause of cancer-related death among both men and women around the world. A lot of research attention has been attracted to diagnosing and treating lung cancer. A common method of lung cancer treatment is based on COX-2 (Cyclooxygenase-2) inhibitors. This is because COX-2 is commonly over expressed in lung cancer and also the abundance of its enzymatic product Prostaglandin E2 (PGE2). Instead of using traditional COX-2 inhibitors to treat lung cancer, here, we report a new anti-cancer strategy recently developed for lung cancer treatment. It adopts more abundant omega-6 (ω-6)fatty acids such as dihomo-γ-linolenic acid (DGLA) in the daily diet and the commonly high levels of COX expressed in lung cancer to promote the formation of 8-hydroxyoctanoic acid (8-HOA) through a new delta-5-desaturase (D5Di) inhibitor. The D5Di will not only limit the metabolic product, PGE2 but also promote the COX-2 catalyzed DGLA peroxidation to form 8-HOA, a novel anti-cancer free radical byproduct. Therefore, the measurement of the PGE2 and 8-HOA levels in cancer cells can be an effective method to treat lung cancer by providing in-time guidance. A novel sensor based on a newly developed functionalized nanomaterial, 2-dimensional nanosheets, Ti3C2 MXene, has proved to sensitively, selectively, precisely and effectively detect PGE2 and 8-HOA in A549 lung cancer cells. Due to the multilayered structure and extremely large surface area, metallic conductivity and easy and versatile in surface modification, Ti3C2 MXene-based sensor will be able to selectively adsorb different molecules through physical adsorption or electrostatic attraction, and lead to a measurable change in the conductivity of the material with high signal-to-noise ratio and excellent sensitivity.

1. Introduction

The most common cancers occur in lungs, breast, pancreas, colon, skin and stomach [1]. Lung cancer is the second most common cancer in men and women and the first leading cause of cancer deaths in the United States. The two major types of lung cancer are: small cell lung cancer (SCLC, ~15%) [2] and non-small cell lung cancer (NSCLC, ~85%) [3]. The survival rate of both types of lung cancer is very low [4]. According to the American Cancer Society, lung cancer and asbestos-related lung cancer [5] alone are responsible for 142,670 estimated deaths in 2019, making it the number one killer and three times deadlier than breast cancer [6]. This is because most patients (~75%) have been diagnosed at a late stage of the disease (stage III or IV) [7].To increase the survival rate, major advances in lung cancer control or prevention will be greatly aided by early detection and effective anti-cancer therapy. In recent years, a variety of therapeutic and adjuvant methods and nutritional approaches have been developed for lung cancer treatment such as chemotherapy, targeted therapy [8,9], cyclooxygenase (COX)-2 inhibition [10], and omega-3 fatty acid dietary manipulation [11,12].
Besides these methods, many physical ‘visualization/detection’ methods [13] are available for tumor detection and cancer diagnosis [14]. Some of them are Positron Emission Tomography (PET), Magnetic Resonance Imaging (MRI), Computerized Tomography (CT), Ultrasonography, Endoscopy and Gas Chromatography method. However, these methods have some major issues for applications in cancer diagnosis. For example, MRI is very expensive and time-consuming. Sometimes it even cannot distinguish between malignant and benign cancer [15]. In case of PET, radioactive material is used which is combined with glucose and injected into the patient. This might be a health concern for diabetic patients [16]. High-dose radiation involved in CT scanning can even increase the risk of cancer [17]. Ultrasound, however, cannot provide accurate diagnosis and frequently has trouble to determine whether a mass is malignant or not [18]. Endoscopy is relatively safer but still has complications like perforation, infection, bleeding and pancreatitis [19].The fundamental limitation of gas chromatography is that the substance must be volatile. It means that a finite portion of the substance needs to be distributed into the gaseous state [20]. It is problematic to use Gas Chromatography Mass Spectroscopy (GC-MS) in cancer detection because its sampling procedure is very complicated and the results are difficult to interpret. This technique is very expensive and must be operated by a very skilled personnel [21]. Therefore, an effective and accurate technique to detect tumor and diagnose cancer is urgently needed.
In particular, studies have confirmed that cyclooxygenase (COX), typically the inducible form COX-2, is commonly over expressed in lung cancer and the abundance of its enzymatic product prostaglandin E2 (PGE2) plays an important role in influencing cancer development. Since PGE2 is a deleterious metabolite formed from COX-2-catalyzed peroxidation of an upstream omega-6 (ω-6) fatty acid called arachidonic acid (AA), PGE2 promotes tumor growth and metastasis [22]. So, it can be taken as an indicator of local COX activity to regulate or control lung cancer. Many efforts on treating lung cancer have been focused on the development of COX-2 inhibitors because they can be used to suppress prostaglandin E2 (PGE2) formation from COX-2-catalyzed ω-6 arachidonic acid peroxidation [23].However, most COX-2 inhibitors can severely injure the gastrointestinal tract, increase the risk of cardiovascular disease, and provide limited clinical responses [22,23].To seek a safer and more efficient method to treat cancers, a new anti-cancer strategy [24], as shown in Figure 1, has been recently developed which is a very different approach than the classic COX-2 inhibitors [24,25,26]. In detail, this is a strategy which adopts more abundant ω-6s such as dihomo-γ-linolenic acid (DGLA) in the daily diet and the commonly high level of COX expressed in most cancers to promote the formation of 8-hydroxyoctanoic acid (8-HOA) through using a newly developed inhibitor, delta-5-desaturase (D5Di) inhibitor. This is because the D5D is an enzyme that converts an upstream DGLA in diet to AA. The high expression of COX-2 will promote the conversion of AA to PGE2, while the D5Di will 1) knock down the conversion of DGLA to AA and limit the metabolic product, PGE2; 2) promote the COX-2 catalyzed DGLA peroxidation to form 8-HOA, a novel anti-cancer free radical by-product. This strategy has proven to produce more effective and safer therapeutic outcomes in cancer treatment and be validated in the colon and pancreatic cancers [27]. Therefore, detection of the PGE2 and 8-HOA in lung cancer should be an effective method to evaluate the efficiency of the cancer treatment. Furthermore, the relative ratio of PGE2 and 8-HOA concentrations can become a useful adjuvant method to help diagnose cancers at an early stage. Therefore, it is very critical to develop a technique or device, which can track PGE2 and 8-HOA concentrations in cancer and provide in-time guidance and feedback for cancer treatment and prevention. However, due to the extremely low concentrations of PGE2 and 8-HOA in cancer cells ~ ng/mLor µM, the detection of these components is quite challenging. The traditional methods to measure low concentration of compounds, such as PGE2 and 8-HOA, are using gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-mass spectrometry (LC-MS). These techniques, as described above, are accurate and sensitive but heavy (not portable), expensive (skilled personnel to operate) and time-consuming. Recent advances in nanofabrication technology have made it possible to be widely used in electronics, sensing, biomaterials for variable areas including disease diagnosis and control in medicine, drug delivery, and food industry [28,29,30,31]. Due to the increased surface areas and the feasibility of controllable size and surface properties, nanomaterials such as nanofibers, nanowires, nanoparticles provide great opportunities for the development of advanced sensing systems and portable device/instrumentation with improved sensitivity, and selectivity [32,33,34,35,36,37,38]. In particular, the use of structure-directing synthetic approaches in nanomaterial synthesis allows the preparation of particularly promising nanomaterials by tailoring nanomaterial crystalline phase, surface states, morphology, and facets for specific sensing application. With the development of two-dimensional (2D) nanomaterials such as graphene, these type of materials have gained tremendous attention because of their astonishing electrical and optical properties featured with “all-surface” nature [39,40,41,42,43]. Such all-surface nature can offer great opportunities to tailor material properties through surface treatment for targetable detection. In 2011, the birth of MXenes introduced a new family into the two-dimensional (2D) materials and further proves to be promising in the flexible sensory application due to its controllable preparation method and fascinating properties. In essence, MXenes consists of transition metals (including Ti, V, Nb, Mo, etc.) and carbon or nitrogen, sharing a general formula of Mn+1Xn (n =1–9). As a new star of 2D materials, MXenes have the metallic conductivity and hydrophilic nature due to their uncommon surface terminations. The unique accordion-like morphology (Figure 2), excellent conductivity, and rich but tailorable surface functional groups endow MXenes with attractive electronic, mechanical, physical and chemical properties for applications in energy storage [44], environmental science [45] and sensors [46]. In this paper, we report a new sensor based on a newly developed 2-dimentional nanomaterial, Ti3C2 MXene. The preliminary data indicate that this device can sensitively detect PGE2 and 8-HOA levels in healthy and cancerous lung cells (BEAS2B and A549 respectively) in order to validate the effectivity of this new strategy in lung cancer treatment as well as in-time guidance during the therapy.

2. Materials and Methods

2.1. Sensing Material Synthesis and Cell Lines Preparation

2.1.1. Ti3C2 Nanomaterial-Based Sensor Preparation

The sensor that we used is based on a new 2-D nanomaterial, Ti3C2 MXene. This nanomaterial was prepared using a method developed in our group, named as the‘hot etching method’ [47,48]. In details, the synthesis of Ti3C2MXene followed the steps: (1) Preparing Ti3AlC2MAX phase. It was obtained through ball milling TiC, Ti, Al powders in the molar ratio 2:1:1.2 for 5 h. Under argon flow, the resulting powder was then pressed into a pellet and sintered at 1350 °C for 4 h. The collected pellet after being milled back into powder was sieved through a 160-mesh sieve; (2) Etching Al from MAX phase to form MXene phase. The as-prepared MAX powder was collected at an elevated temperature for etching through‘hot-etch method’. HF acid in 25 mL Teflon line autoclave at a temperature of 150 °C was used in a Thermolyne furnace for 5 h to etch 0.5 g of MAX phase. To remove Al from MAX phase, 5 % wt of HF were used. Materials after being sonicated for one hour using a sonicating bath were collected through centrifuge; all the materials were dried overnight in a drying oven at 65 °C. (3) Synthesizing MXene powders for the sensing film. Finally, the synthesized nanomaterial was drop-casted on the gold electrode patterned glass substrate to form athin film. The morphology of the synthesized 2D multilayered nanomaterial is shown in Figure 2a as the scanning electron microscope (SEM) image, which clearly exhibits multilayered nanosheets and accordion-like morphology. Figure 2b reveals the as-synthesizedTi3C2material’s special surface terminations, which can lead to unique surface and material properties of Ti3C2.

2.1.2. Cancer Cell Lines and Materials

A549 (ATCC®CCL-185™), and BEAS-2B (ATCC®CRL-9609™) were purchased from American Type Culture Collection (ATCC, VA, USA). Iminodibenzyl (CAS Number: 494-19-9) and8-hydroxyoctanoic acid (8-HOA) were obtained from Sigma-Aldrich (St. Louis, MO, USA). PGE2andDGLA (forin vitrostudy), DGLA ethyl ester (forin vivostudy), were acquired from Cayman Chemical (MI, USA).

2.1.3. Preparation of Cell Samples

About 3 × 105 A549 or BEAS-2B cells were trypsinized and seeded into each well of the 6-well plates. Then the cells were randomly assigned into different groups for the administration of DGLA (100 μM), iminodibenzyl (10 μM), or their combination accordingly. After 48 hours, the cell culture medium was collected. Cells were washed with PBS and collected by centrifugation after trypsinization. A 1 mL cell culture medium with collected cells was homogenate and ready for testing. Three different groups of control samples were prepared using the same preparation procedures, including (a) blank group in 1 mL cell homogenate without any treatment; (b) 8-HOA group in 1 mL cell homogenate containing 0.6 ug/mL exogenous 8-HOA; (c) PGE2 group in 1 mL cell homogenate containing 6 ug/mL exogenous PGE2.

2.1.4. Xenografted Lung Tumor Model on Nude Mice

Six-week-old nude mice (nu/nu) mice were purchased from The Jackson Laboratory. The mice were housed in a pathogen-free IVC System with water and food ad libitum. All the animal experiments in this study were approved by the Institutional Animal Care and Use Committees at North Dakota State University. About 2 × 106 A549 cells were injected into the hind flank of the nude mouse to induce tumors as we previously described [26]. The mice were randomly assigned to the following treatments: Control (treated with the same volume of the vehicle), DGLA (5 mg/mouse, oral gavage, every day), iminodibenzyl (15 mg/kg, intraperitoneal injection, every day) and DGLA+ iminodibenzyl. The treatment was started at two weeks of injection of A549 cells in nude mice. All the administrations lasted for four weeks. At end of the treatment, mice were sacrificed and tumors were isolated. Tumor tissues were crushed and homogenized by using a mortar in liquid nitrogen. The blood was centrifuged for 10 min at 2000 rpm for separating serum. The supernatant of tumor tissues and serum was collected for analysis.

2.2. Methodology

To verify the roles of 8-HOA and PGE2 in cancer development and treatment, the experiments have been designed to do testing in healthy lung cells and A549 lung cancer cells.

2.2.1. Normal Cells

For application to test the effect of 8-HOA and PGE2 in normal lung cells, 106 BEAS2B non-tumerogenic epithelial cell lines were collected. 8-HOA, PGE2 and BSA (Bovine Serum Albumin) were applied to the samples right before measuring the resistance change. Once the samples were applied onto the Ti3C2MXene-based sensors, resistances were measured immediately and repeated at regular time intervals. The experiment is listed in the Table 1and the resistance change of the MXene slides for each of the samples is measured and shown in Figure 3. The resistance increases dramatically when BEAS2B with PGE2 is added but BEAS2B alone and BEAS2B with 8-HOA does not show obvious change of resistance.

2.2.2. A549 lung Cancer Cells

A549 lung cancer epithelial cell lines were collected after being cultured. Similar to the BEAS-2B cell lines, 8-HOA and PGE2 samples were applied to the A549 cell lines just before conducting the experiment. The complete design of the experiments to verify the relative concentration of generated 8-HOA and PGE2 with and without using the new cancer treatment are listed in the Table 2.
The sensing tests of these samples are shown in Figure 4. The resistances of A549 cancer cells, A549 cells treated by DGLA, and PGE2 are much higher than the cancer cells treated by adding 8-HOA, applying D5Di, or using the new anti-cancer treatment DGLA + D5Di.

3. Results and Discussion

3.1. Observation from the Non-Tumerogenic Sample Graph

In a healthy subject, both the concentration of PGE2 and 8-HOA should be low. The sensing test is conducted on the normal lung cell, BEAS2B without extra treatment and BEAS2B by treating with extra PGE2 or 8-HOA. Significant resistance increase is observed in BEAS2B by adding 10 µM PGE2 while the untreated normal cells and cells treated by 8-HOA do not show obvious resistance change. This result indicates a unique role of PGE2 in cells through the change of electrical property of cells. Considering the elevated concentration of PGE2 can indicate a cancer development, such a sensitive response to PGE2 using Ti3C2 MXene-based sensor can be potentially used to diagnose cancer even at a very early stage.

3.2. Observation from the CARCINOGENIC Samples (1st Trial)

As we have discussed in this paper previously, D5D inhibitor (D5Di) is used for preventing the conversion of DGLA to AA and ultimately limiting the formation of PGE2.According to the main mechanism of the new anti-cancer strategy, D5Di along with DGLA can effectively limit the formation of PGE2 but promote the formation of 8-HOA. The sensing test using the newly developed Ti3C2 MXene-based sensor, as shown in Figure 4, exhibits an interesting trend of resistance change. Similar to showing high resistance for A549, A549 with adding 10uM PGE2, and A549 treated by DGLA both show high resistance as well. The results indicate a higher concentration of PGE2 generated in A549 cells just by using DGLA, which confirms that omega-6 (DGLA) are pro-inflammatory and promote the formation of PGE2. However, the new anti-cancer treatment using DGLA and D5Di to treat A549 cells shows the similar resistance level as that of A549 cells with 8-HOA. This result provides promising information: the Ti3C2 MXene-based sensor can be used to monitor or validate the anti-cancer effect of the new strategy: DGLA + D5Di, which should be an effective anti-cancer effect due to the generation of 8-HOA. This result is consistent with the results using GC-MS to confirm the new anti-cancer effect, as shown in Figure 5 [49].

4. Conclusions and Discussion

The preliminary results lead to an important conclusion: the Ti3C2-based sensor can be used as a convenient and simple method tool for anti-cancer treatment guidance and monitoring to sensitively detect the trace concentrations of PGE2 and 8-HOA. Instead of using heavy, expensive and time-consuming GC-MS to assist the anti-cancer treatment, Ti3C2 MXene based sensor can provide a faster, easier, efficient and much less invasive assistant tool to detect and cure cancer.

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  49. Pang, L.; Shah, H.; Zhao, P.; Qian, S.; Sathish, V. New Delta-5-Desaturase Inhibitor Suppress Lung Cancer Progression: A Paradigm Shift on COX-2 Biology in Lung Cancer Treatment. FASEB J. 2020, 34, 1. [Google Scholar] [CrossRef]
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Figure 1. New anti-cancer strategy: target but not inhibit COX-2 in cancer.
Figure 1. New anti-cancer strategy: target but not inhibit COX-2 in cancer.
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Figure 2. Newly synthesized 2D multi-layered Ti3C2 MXene nanosheets (a) Scanning Electron Microscope (SEM) image; (b) Pristine and surface-terminated Ti3C2MXene with different functional groups.
Figure 2. Newly synthesized 2D multi-layered Ti3C2 MXene nanosheets (a) Scanning Electron Microscope (SEM) image; (b) Pristine and surface-terminated Ti3C2MXene with different functional groups.
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Figure 3. Resistance change measured using Ti3C2 MXene-based sensors for BEAS2B cells.
Figure 3. Resistance change measured using Ti3C2 MXene-based sensors for BEAS2B cells.
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Figure 4. Resistance change measured using Ti3C2 MXene-based sensors for A549 cancer cells with and without using the new anti-cancer treatment.
Figure 4. Resistance change measured using Ti3C2 MXene-based sensors for A549 cancer cells with and without using the new anti-cancer treatment.
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Figure 5. Effect of DGLA and D5D siRNA on 8-HOA formation.
Figure 5. Effect of DGLA and D5D siRNA on 8-HOA formation.
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Table
Table 1. Table showing the composition of each sample for BEAS2B.
Table 1. Table showing the composition of each sample for BEAS2B.
SampleCell8-HOAPGE2BSA
1106 BEAS2BnonenoneNone
2106 BEAS2B0.6 ug/mLnoneNone
3106 BEAS2Bnone6 ug/mLNone
4Nonenonenone1 mg/mL
Table
Table 2. The composition of each sample for A549 cells treated by 8-hydroxyoctanoic acid (8-HOA), Prostaglandin E2 (PGE2), dihomo-γ-linolenic acid (DGLA), delta-5-desaturase (D5Di) and DGLA + D5Di.
Table 2. The composition of each sample for A549 cells treated by 8-hydroxyoctanoic acid (8-HOA), Prostaglandin E2 (PGE2), dihomo-γ-linolenic acid (DGLA), delta-5-desaturase (D5Di) and DGLA + D5Di.
SampleCellDGLAD5Di8-HOAPGE2Estimated 8-HOA/PGE2Level
1106 A549nonenonenonenoneLow 8-HOA; low PGE2
2106 A549nonenone0.6 ug/mLnoneHigh 8-HOA; low PGE2
3106 A549nonenonenone6 ug/mLLow 8-HOA; high PGE2
4106 A549100 uMnonenonenoneLow 8-HOA; high PGE2
5106 A549none10 uMnonenoneLow 8-HOA; low PGE2
6106 A549100 uM10 uMnonenoneHigh 8-HOA; low PGE2
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Sadiq, M.; Pang, L.; Johnson, M.; Sathish, V.; Wang, D. 2D Nanomaterial, Ti3C2 MXene-Based Sensor to Guide Lung Cancer Therapy and Management. Proceedings 2020, 60, 29. https://doi.org/10.3390/IECB2020-07055

AMA Style

Sadiq M, Pang L, Johnson M, Sathish V, Wang D. 2D Nanomaterial, Ti3C2 MXene-Based Sensor to Guide Lung Cancer Therapy and Management. Proceedings. 2020; 60(1):29. https://doi.org/10.3390/IECB2020-07055

Chicago/Turabian Style

Sadiq, Mahek, Lizhi Pang, Michael Johnson, Venkatachalem Sathish, and Danling Wang. 2020. "2D Nanomaterial, Ti3C2 MXene-Based Sensor to Guide Lung Cancer Therapy and Management" Proceedings 60, no. 1: 29. https://doi.org/10.3390/IECB2020-07055

APA Style

Sadiq, M., Pang, L., Johnson, M., Sathish, V., & Wang, D. (2020). 2D Nanomaterial, Ti3C2 MXene-Based Sensor to Guide Lung Cancer Therapy and Management. Proceedings, 60(1), 29. https://doi.org/10.3390/IECB2020-07055

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