Electrochemical Impedance Spectroscopy Characterization of Label-Free Biosensors for the Detection of HER2 in Saliva †
Abstract
:1. Introduction
2. Materials and Method
2.1. Chemical Products
2.2. Antibodies (Ab) and Antigen (Ag) Solutions
2.3. Screen-Printed Electrodes
2.4. Electrochemical Characterization
2.5. Bio-Functionalization of the WE
2.6. HER2 Detection in Real Saliva
3. Results
3.1. Detection and Interferences of HER2 in PBS
3.2. Detection of HER2 in Real Saliva
4. Discussion
4.1. Bode Plot and the Equivalent Circuit
4.2. Detection of HER2 in PBS
4.3. Detection of HER2 in Saliva
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
- Pilleron, S.; Sarfati, D.; Janssen-Heijnen, M.; Vignat, J.; Ferlay, J.; Bray, F.; Soerjomataram, I. Global cancer incidence in older adults, 2012 and 2035: A population-based study. Int. J. Cancer 2018, 58, 49–58. [Google Scholar] [CrossRef] [PubMed]
- Torre, L.A.; Bray, F.; Siegel, R.L.; Ferlay, J.; Lortet-tieulent, J.; Jemal, A. Global Cancer Statistics, 2012. CA Cancer J. Clin. 2015, 65, 87–108. [Google Scholar] [CrossRef] [PubMed]
- Ferlay, J.; Colombet, M.; Soerjomataram, I.; Mathers, C.; Parkin, D.M.; Piñeros, M.; Znaor, A.; Bray, F. Estimating the global cancer incidence and mortality in 2018: GLOBOCAN sources and methods. Int. J. Cancer 2018, 1–13. [Google Scholar] [CrossRef]
- Duffy, M.J.; Walsh, S.; McDermott, E.W.; Crown, J. Biomarkers in Breast Cancer: Where Are We and Where Are We Going? 1st ed.; Elsevier Inc.: Amsterdam, The Netherlands, 2015; Volume 71, ISBN 9780128022566. [Google Scholar]
- Nicolini, A.; Ferrari, P.; Duffy, M.J. Prognostic and predictive biomarkers in breast cancer: Past, present and future. Semin. Cancer Biol. 2018, 52, 56–73. [Google Scholar] [CrossRef]
- Rakha, E.A.; Green, A.R. Molecular classification of breast cancer: What the pathologist needs to know. Pathology 2017, 49, 111–119. [Google Scholar] [CrossRef]
- Omenn, G.S.; Guan, Y.; Menon, R. A new class of protein cancer biomarker candidates: Differentially expressed splice variants of ERBB2 (HER2/neu) and ERBB1 (EGFR) in breast cancer cell lines. J. Proteomics 2014, 107, 103–112. [Google Scholar] [CrossRef]
- Ferreira, P.M.P.; Pessoa, C. Molecular biology of human epidermal receptors, signaling pathways and targeted therapy against cancers: New evidences and old challenges. Brazilian J. Pharm. Sci. 2017, 53. [Google Scholar] [CrossRef]
- Greabu, M.; Battino, M.; Mohora, M.; Totan, A.; Didilescu, A.; Spinu, T.; Totan, C.; Miricescu, D.; Radulescu, R. Saliva—A diagnostic window to the body, both in health and in disease. J. Med. Life 2009, 2, 124–132. [Google Scholar] [PubMed]
- Liu, J.; Duan, Y. Saliva: A potential media for disease diagnostics and monitoring. Oral Oncol. 2012, 48, 569–577. [Google Scholar] [CrossRef] [PubMed]
- Abrao Nemeir, I.; Saab, J.; Hleihel, W.; Errachid, A.; Jafferzic-Renault, N.; Zine, N. The Advent of Salivary Breast Cancer Biomarker Detection Using Affinity Sensors. Sensors 2019, 19, 2373. [Google Scholar] [CrossRef]
- Malon, R.S.P.; Sadir, S.; Balakrishnan, M.; Córcoles, E.P. Saliva-Based Biosensors: Noninvasive Monitoring Tool for Clinical Diagnostics. Biomed Res. Int. 2014, 2014, 1–20. [Google Scholar] [CrossRef] [PubMed]
- Chojnowska, S.; Baran, T.; Wilińska, I.; Sienicka, P.; Cabaj-Wiater, I.; Knaś, M. Human saliva as a diagnostic material. Adv. Med. Sci. 2018, 63, 185–191. [Google Scholar] [CrossRef]
- Yordanova, M.G.; Gerova, D.I.; Galunska, B.T. Saliva application in oral and systemic diseases. Scr. Sci. Medica 2018, 50, 13–18. [Google Scholar] [CrossRef]
- Streckfus, C.; Bigler, L.; Dellinger, T.; Dai, X.; Kingman, A.; Thigpen, J.T. The presence of soluble c-erbB-2 in saliva and serum among women with breast carcinoma: A preliminary study. Clin. Cancer Res. 2000, 6, 2363–2370. [Google Scholar] [PubMed]
- Streckfus, C.F.; Arreola, D.; Edwards, C.; Bigler, L. Salivary protein profiles among her2/neu-receptor-positive and -negative breast cancer patients: Support for using salivary protein profiles for modeling breast cancer progression. J. Oncol. 2012, 2012, 1–9. [Google Scholar] [CrossRef]
- Laidi, F.; Bouziane, A.; Lakhdar, A.; Khabouze, S.; Rhrab, B.; Zaoui, F. Salivary expression of soluble HER2 in breast cancer patients with positive and negative HER2 status. OncoTargets Ther. 2014, 7, 1285–1289. [Google Scholar] [CrossRef] [PubMed]
- Chiu, M.L.; Lawi, W.; Snyder, S.T.; Wong, P.K.; Liao, J.C.; Gau, V. Matrix Effects-A Challenge Toward Automation of Molecular Analysis. JALA J. Assoc. Lab. Autom. 2010, 15, 233–242. [Google Scholar] [CrossRef]
- Jayanthi, V.S.P.K.S.A.; Das, A.B.; Saxena, U. Recent advances in biosensor development for the detection of cancer biomarkers. Biosens. Bioelectron. 2017, 91, 15–23. [Google Scholar] [CrossRef] [PubMed]
- Barhoumi, L.; Baraket, A.; Bellagambi, F.G.; Karanasiou, G.S.; Ali, M.B.; Fotiadis, D.I.; Bausells, J.; Zine, N.; Sigaud, M.; Errachid, A. A novel chronoamperometric immunosensor for rapid detection of TNF-A in human saliva. Sens. Actuators B Chem. 2018, 266, 477–484. [Google Scholar] [CrossRef]
- Baraket, A.; Lee, M.; Zine, N.; Sigaud, M.; Bausells, J.; Errachid, A. A fully integrated electrochemical biosensor platform fabrication process for cytokines detection. Biosens. Bioelectron. 2017, 93, 170–175. [Google Scholar] [CrossRef]
- Bahadir, E.B.; Sezgintürk, M.K. A review on impedimetric biosensors. Artif. Cells, Nanomedicine Biotechnol. 2016, 44, 248–262. [Google Scholar] [CrossRef] [PubMed]
- Randviir, E.P.; Banks, C.E. Electrochemical impedance spectroscopy: An overview of bioanalytical applications. Anal. Methods 2013, 5, 1098–1115. [Google Scholar] [CrossRef]
- Karunakaran, C.; Rajkumar, R.; Bhargava, K. Introduction to Biosensors. Biosens. Bioelectron. 2015, 60, 1–68. [Google Scholar]
- Taleat, Z.; Khoshroo, A.; Mazloum-Ardakani, M. Screen-printed electrodes for biosensing: A review (2008-2013). Microchim. Acta 2014, 181, 865–891. [Google Scholar] [CrossRef]
- Barhoumi, L.; Bellagambi, F.G.; Vivaldi, F.M.; Baraket, A.; Clément, Y.; Zine, N.; Ali, M.B.; Elaissari, A.; Errachid, A. Ultrasensitive immunosensor array for TNF-α detection in artificial saliva using polymer-coated magnetic microparticles onto screen-printed gold electrode. Sensors 2019, 19, 692. [Google Scholar] [CrossRef]
- Radi, A.E.; Lates, V.; Marty, J.L. Mediatorless hydrogen peroxide biosensor based on horseradish peroxidase immobilized on 4-carboxyphenyl film electrografted on gold electrode. Electroanalysis 2008, 20, 2557–2562. [Google Scholar] [CrossRef]
- Campuzano, S.; Yánez-Sedeño, P.; Pingarrón, J.M. Electrochemical bioaffinity sensors for salivary biomarkers detection. TrAC Trends Anal. Chem. 2017, 86, 14–24. [Google Scholar] [CrossRef]
- Abrao Nemeir, I.; Saab, J.; Hleihel, W.; Errachid, A.; Zine, N. Impedimetric label-free detection of salivary EGFR on screen printed electrode. Integr. Cancer Sci. Ther. 2019, 6, 1–3. [Google Scholar] [CrossRef]
- Huang, J.; Li, Z.; Liaw, B.Y.; Zhang, J. Graphical analysis of electrochemical impedance spectroscopy data in Bode and Nyquist representations. J. Power Sources 2016, 309, 82–98. [Google Scholar] [CrossRef]
- Nouira, W.; Maaref, A.; Vocanson, F.; Siadat, M.; Saulnier, J.; Lagarde, F.; Jaffrezic-Renault, N. Enhancement of Enzymatic IDE Biosensor Response Using Gold Nanoparticles. Example of the Detection of Urea. Electroanalysis 2012, 24, 1088–1094. [Google Scholar] [CrossRef]
- Lasia, A. Electrochemical Impedance Spectroscopy and Its Applications; Springer: New York, NY, USA, 2014; Volume 9781461489337, ISBN 9781461489337. [Google Scholar]
- Sierke, S.L.; Cheng, K.; Kim, H.H.; Koland, J.G. Biochemical characterization of the protein tyrosine kinase homology domain of the ErbB3 (HER3) receptor protein. Biochem. J. 1997, 322, 757–763. [Google Scholar] [CrossRef] [PubMed]
- Bellagambi, F.G.; Baraket, A.; Longo, A.; Vatteroni, M.; Zine, N.; Bausells, J.; Fuoco, R.; Di Francesco, F.; Salvo, P.; Karanasiou, G.S.; et al. Electrochemical biosensor platform for TNF-α cytokines detection in both artificial and human saliva: Heart failure. Sens. Actuators B Chem. 2017, 251, 1026–1033. [Google Scholar] [CrossRef]
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Nemeir, I.A.; Mouawad, L.; Saab, J.; Hleihel, W.; Errachid, A.; Zine, N. Electrochemical Impedance Spectroscopy Characterization of Label-Free Biosensors for the Detection of HER2 in Saliva. Proceedings 2020, 60, 17. https://doi.org/10.3390/IECB2020-07081
Nemeir IA, Mouawad L, Saab J, Hleihel W, Errachid A, Zine N. Electrochemical Impedance Spectroscopy Characterization of Label-Free Biosensors for the Detection of HER2 in Saliva. Proceedings. 2020; 60(1):17. https://doi.org/10.3390/IECB2020-07081
Chicago/Turabian StyleNemeir, Imad Abrao, Lynn Mouawad, Joseph Saab, Walid Hleihel, Abdelhamid Errachid, and Nadia Zine. 2020. "Electrochemical Impedance Spectroscopy Characterization of Label-Free Biosensors for the Detection of HER2 in Saliva" Proceedings 60, no. 1: 17. https://doi.org/10.3390/IECB2020-07081