Antioxidant Properties and Cytoprotective Effect Against H2O2-Induced Cytotoxicity in Mouse Fibroblasts Cells (L-929) of Horseradish Leaves †
by
, and
Ramona-Daniela Păvăloiu
1,2, 
Fawzia Sha’at
1,2,*,
Corina Bubueanu
1,
Georgeta Neagu
1,
Cristina Hlevca
1,
Mousa Sha’at
3 and
Gheorghe Nechifor
2 1
National Institute for Chemical-Pharmaceutical Research and Development (ICCF) Bucharest,112 Vitan Av. 31299 Bucharest, Romania
2
Faculty of Applied Chemistry and Material Science, University Politehnica of Bucharest, 060042 Bucharest, Romania
3
Faculty of Pharmacy, University of Medicine and Pharmacy, Gr. T. Popa, 700115 Iasi, Romania
*
Author to whom correspondence should be addressed.
†
Presented at the 15th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 30th October–1st November 2019.
Proceedings 2019, 29(1), 30; https://doi.org/10.3390/proceedings2019029030
Published: 12 October 2019
(This article belongs to the Proceedings of Priorities of Chemistry for a Sustainable Development-PRIOCHEM)
Horseradish (Armoracia rusticana L.) is a perennial plant from the Brassicaceae family native to Europe and Asia, and globally widespread through cultivation. Horseradish contains phenolic compounds (flavonoids and phenolic acids), vitamins (C and B1), minerals (iron, potassium, calcium and magnesium), and essential oils (sinigrin) [1,2]. Roots, leaves and/or isolated compounds from horseradish possess antioxidant, antimicrobial, chemopreventive, anti-inflammatory, gastroprotective and hypocholesterolaemic activities [3,4]. The aim of this paper was to investigate the antioxidative activity, in vitro citotoxicity, and in vitro cytoprotective effect of A. rusticana (leaves) against H2O2-induced cytotoxicity in mouse fibroblasts cells (L-929).
Plant material—leaves of A. rusticana—were harvested from Dambovita County, Romania, Europe. The antioxidant activity was assessed using the Sanchez-Moreno assay [5]. In vitro cytotoxicity of the plant extract on L-929 murine fibroblast cell line was evaluated through MTS method. The protective effect of A. rusticana against oxidative stress on L-929 mouse fibroblast cells was performed by pretreatment with different concentrations of A. rusticana (5, 10, 25 μg/mL) for one hour and for 24 h before exposing to H2O2 to induce oxidative stress.
The present investigation showed that A. rusticana extracts have a moderate cytotoxic activity (IC50 = 70.40 ± 0.305 µg/mL) and a significant antioxidant effect. A prolonged pretreatment (24 h) with A. rusticana extract was able to protect L-929 murine fibroblast cells against H2O2-induced cytotoxicity, while in the case of the short-term pretreatment (one hour) almost no effect was observed.
The results suggest that horseradish leaves exert great potential for the development of dietary supplements.
Acknowledgments
This work was supported by the Ministry of Education and Scientific Research, grant numbers: PN 19-41 041 01/ 2019 and PN 1627-03-02.
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MDPI and ACS Style
Păvăloiu, R.-D.; Sha’at, F.; Bubueanu, C.; Neagu, G.; Hlevca, C.; Sha’at, M.; Nechifor, G. Antioxidant Properties and Cytoprotective Effect Against H2O2-Induced Cytotoxicity in Mouse Fibroblasts Cells (L-929) of Horseradish Leaves. Proceedings 2019, 29, 30. https://doi.org/10.3390/proceedings2019029030
AMA Style
Păvăloiu R-D, Sha’at F, Bubueanu C, Neagu G, Hlevca C, Sha’at M, Nechifor G. Antioxidant Properties and Cytoprotective Effect Against H2O2-Induced Cytotoxicity in Mouse Fibroblasts Cells (L-929) of Horseradish Leaves. Proceedings. 2019; 29(1):30. https://doi.org/10.3390/proceedings2019029030
Chicago/Turabian StylePăvăloiu, Ramona-Daniela, Fawzia Sha’at, Corina Bubueanu, Georgeta Neagu, Cristina Hlevca, Mousa Sha’at, and Gheorghe Nechifor. 2019. "Antioxidant Properties and Cytoprotective Effect Against H2O2-Induced Cytotoxicity in Mouse Fibroblasts Cells (L-929) of Horseradish Leaves" Proceedings 29, no. 1: 30. https://doi.org/10.3390/proceedings2019029030
