Next Article in Journal
Quantum Computer—A New Technology for the Information Age
Previous Article in Journal
Flow Cytometry and FTIR Spectroscopy for Detection of Early Apoptosis in Human T Cells
Article Menu
Issue 25 (Cell Death 2018) cover image

Export Article

Open AccessProceedings
Proceedings 2018, 2(25), 1529; https://doi.org/10.3390/proceedings2251529

Investigation of the Relation between Follicular Atresia and Granulosa Cells in Terms of Cell Death Mechanisms in Premature Ovarian Failure Model

1
Department of Histology & Embryology, Institute of Medical Sciences, Manisa Celal Bayar University, Manisa 45030, Turkey
2
Department of Histology & Embryology, Faculty of Medicine, Manisa Celal Bayar University, Manisa 45030, Turkey
3
Experimental Health Sciences Research Center, Near East University, Nicosia 99138, Cyprus
Presented at the 2nd International Cell Death Research Congress, Izmir, Turkey, 1–4 November 2018.
*
Author to whom correspondence should be addressed.
Published: 6 December 2018
PDF [321 KB, uploaded 6 December 2018]

Abstract

Premature Ovarian Failure; is characterized by the dysfunction or early depletion of ovarian reserves due to follicular loss in the ovary in women under age of 40. POF is the important cause of infertility and its etiology is still not clearly understood. Investigation of cell death mechanisms (CDM) that play a role in the follicular atresia (FA) triggered by excessive loss of granulosa cells (GCs) that provide metabolic support for oocyte and follicle development in the ovary will help to understand POF etiology. It was known that apoptosis and autophagy play a role in FA. Recent studies have shown that paraptosis, associated with endoplasmic reticulum stress (ERS), also exist in FA. POF model was established in C57BL/6 female mice by CTX and it was confirmed by increased follicle stimulating hormone (FSH), luteal hormone (LH) and decreased estradiol (E2) blood levels and follicle count. According to the results of the immunohistochemistry (IHC) cell death markers were significantly more expressed than control (C) and sham (S) groups in the POF model. In addition, more apoptotic cells were observed in the POF group compared to C and S in the TUNEL analysis. In consequence of this study apoptosis and autophagy as well as paraptosis play a role in the FA leading to POF, will help to develop new treatment protocols.
Keywords: Premature Ovarian Failure; cyclophosphamide; follicular atresia; apoptosis; autophagy; paraptosis Premature Ovarian Failure; cyclophosphamide; follicular atresia; apoptosis; autophagy; paraptosis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Akogullari, D.; Uluer, E.T.; Vatansever, H.S. Investigation of the Relation between Follicular Atresia and Granulosa Cells in Terms of Cell Death Mechanisms in Premature Ovarian Failure Model. Proceedings 2018, 2, 1529.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Proceedings EISSN 2504-3900 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top