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Proceeding Paper

Collagenase Biosensor Based on the Degradation of Peptide Cross-Linked Poly(Ethylene Glycol) Hydrogel Films

1
School of Engineering and Materials Science, Queen Mary University of London, London E1 4NS, UK
2
Centre of Foundation Studies, Universiti Teknologi MARA, Cawangan Selangor, Kampus Dengkil, Dengkil 43800, Malaysia
3
Institute of Medical Engineering, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an 710061, China
4
The Lennard-Jones Laboratories, School of Chemical and Physical Sciences, Keele University, Staffordshire ST5 5BG, UK
*
Author to whom correspondence should be addressed.
Presented at the Eurosensors 2018 Conference, Graz, Austria, 9–12 September 2018.
Proceedings 2018, 2(13), 961; https://doi.org/10.3390/proceedings2130961
Published: 30 November 2018
(This article belongs to the Proceedings of EUROSENSORS 2018)
Peptide cross-linked poly(ethylene glycol) hydrogel is a promising biomaterial that has been used widely for drug delivery and tissue engineering. However, the use of this material as a biosensor material for the detection of collagenase has not been explored. Collagenase play a key role in rheumatoid arthritis and osteoarthritis. Detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals and their degradation in the presence of collagenase was monitored using a Quartz Crystal Microbalance (QCM). The biosensor was shown to respond to concentrations between 2 nM to 2000 nM with a lower detection limit of 2 nM.
Keywords: collagenase biosensor; peptide cross-linked; hydrogel; quartz crystal microbalance; degradation; poly(ethylene glycol) collagenase biosensor; peptide cross-linked; hydrogel; quartz crystal microbalance; degradation; poly(ethylene glycol)
MDPI and ACS Style

Ahmad, N.; Colak, B.; Gibbs, M.J.; Zhang, D.-W.; Becer, C.R.; Watkinson, M.; Gautrot, J.E.; Krause, S. Collagenase Biosensor Based on the Degradation of Peptide Cross-Linked Poly(Ethylene Glycol) Hydrogel Films. Proceedings 2018, 2, 961. https://doi.org/10.3390/proceedings2130961

AMA Style

Ahmad N, Colak B, Gibbs MJ, Zhang D-W, Becer CR, Watkinson M, Gautrot JE, Krause S. Collagenase Biosensor Based on the Degradation of Peptide Cross-Linked Poly(Ethylene Glycol) Hydrogel Films. Proceedings. 2018; 2(13):961. https://doi.org/10.3390/proceedings2130961

Chicago/Turabian Style

Ahmad, Norlaily, Burcu Colak, Martin John Gibbs, De-Wen Zhang, C. Remzi Becer, Michael Watkinson, Julien E. Gautrot, and Steffi Krause. 2018. "Collagenase Biosensor Based on the Degradation of Peptide Cross-Linked Poly(Ethylene Glycol) Hydrogel Films" Proceedings 2, no. 13: 961. https://doi.org/10.3390/proceedings2130961

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