Next Article in Journal
How the Chamber Design Can Affect Gas Sensor Responses
Previous Article in Journal
An Open-Source Smart Sensor Architecture for Edge Computing in IoT Applications
Open AccessProceedings

Collagenase Biosensor Based on the Degradation of Peptide Cross-Linked Poly(Ethylene Glycol) Hydrogel Films

1
School of Engineering and Materials Science, Queen Mary University of London, London E1 4NS, UK
2
Centre of Foundation Studies, Universiti Teknologi MARA, Cawangan Selangor, Kampus Dengkil, Dengkil 43800, Malaysia
3
Institute of Medical Engineering, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an 710061, China
4
The Lennard-Jones Laboratories, School of Chemical and Physical Sciences, Keele University, Staffordshire ST5 5BG, UK
*
Author to whom correspondence should be addressed.
Presented at the Eurosensors 2018 Conference, Graz, Austria, 9–12 September 2018.
Proceedings 2018, 2(13), 961; https://doi.org/10.3390/proceedings2130961
Published: 30 November 2018
(This article belongs to the Proceedings of EUROSENSORS 2018)
Peptide cross-linked poly(ethylene glycol) hydrogel is a promising biomaterial that has been used widely for drug delivery and tissue engineering. However, the use of this material as a biosensor material for the detection of collagenase has not been explored. Collagenase play a key role in rheumatoid arthritis and osteoarthritis. Detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals and their degradation in the presence of collagenase was monitored using a Quartz Crystal Microbalance (QCM). The biosensor was shown to respond to concentrations between 2 nM to 2000 nM with a lower detection limit of 2 nM.
Keywords: collagenase biosensor; peptide cross-linked; hydrogel; quartz crystal microbalance; degradation; poly(ethylene glycol) collagenase biosensor; peptide cross-linked; hydrogel; quartz crystal microbalance; degradation; poly(ethylene glycol)
MDPI and ACS Style

Ahmad, N.; Colak, B.; Gibbs, M.J.; Zhang, D.-W.; Becer, C.R.; Watkinson, M.; Gautrot, J.E.; Krause, S. Collagenase Biosensor Based on the Degradation of Peptide Cross-Linked Poly(Ethylene Glycol) Hydrogel Films. Proceedings 2018, 2, 961.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop