Prevalence and Virulent Gene Profiles of Sorbitol Non-Fermenting Shiga Toxin-Producing Escherichia coli Isolated from Goats in Southern Thailand
Abstract
1. Introduction
2. Materials and Methods
2.1. Farm Description and Sample Collection
2.2. Bacteriological Isolation and Primary Identification of Sorbitol Non-Fermenting Shiga Toxin-Producing Escherichia coli (SNF-STEC)
2.3. DNA Extraction
2.4. Serotyping and Virulent Gene Profiling by Molecular Techniques
2.5. Data Analysis
3. Results
3.1. Prevalence of Sorbitol Non-Fermenting STECs (SNF-STECs) and O157 Serotype in Goat Herds
3.2. Virulent Gene Profiles of SNF-STEC Isolates
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primers | Target | Amplicon Size (Base Pair) | Reference |
---|---|---|---|
5′-CGGACATCCATGTGATATGG-3′ | rfb gene for O157 | 259 | [19] |
5′-TTGCCTATGTACAGCTAATCC-3′ | |||
5′-ATAAATCGCCATTCGTTGACTAC-3′ | stx1 | 180 | [19] |
5′-AGAACGCCCACTGAGATCATC-3′ | |||
5′-GGCACTGTCTGAAACTGCTCC-3′ | stx2 | 255 | [19] |
5′-TCGCCAGTTATCTGACATTCTG-3′ | |||
5′-GACCCGGCACAAGCATAAGC-3′ | eaeA | 384 | [20] |
5′-CCACCTGCAGCAACAAGAGG-3′ | |||
5′-GCATCATCAAGCGTACGTTCC-3′ | ehxA | 534 | [20] |
5′-AATGAGCCAAGCTGGTTAAGCT-3′ | |||
5′-CGTGATGAACAGGCTATTGC-3′ | saa | 119 | [20] |
5′-ATGGACATGCCTGTGGCAAC-3′ |
Provinces | Prevalence of (%) | |
---|---|---|
SNF-STECs | O157 Serotype | |
Narathiwat | 7.53 | 1.00 |
Pattani | 2.13 | Not found |
Phatthalung | 31.25 | Not found |
Satun | 15.63 | Not found |
Songkhla | 9.79 | 1.88 |
Yala | 4.17 | Not found |
Isolate’s Name | Farm Location | Virulent Genes | ||||
---|---|---|---|---|---|---|
stx1 | stx2 | eaeA | ehxA | saa | ||
A1.9 | Songkhla | Negative | Positive | Positive | Positive | Negative |
E5.3 | Songkhla | Negative | Positive | Positive | Positive | Negative |
E5.4 | Songkhla | Negative | Positive | Positive | Positive | Negative |
E5.8 | Songkhla | Negative | Positive | Positive | Positive | Negative |
J5.9 | Narathiwat | Negative | Positive | Positive | Positive | Negative |
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Wiriyaprom, R.; Ngasaman, R.; Kaewnoi, D.; Prachantasena, S. Prevalence and Virulent Gene Profiles of Sorbitol Non-Fermenting Shiga Toxin-Producing Escherichia coli Isolated from Goats in Southern Thailand. Trop. Med. Infect. Dis. 2022, 7, 357. https://doi.org/10.3390/tropicalmed7110357
Wiriyaprom R, Ngasaman R, Kaewnoi D, Prachantasena S. Prevalence and Virulent Gene Profiles of Sorbitol Non-Fermenting Shiga Toxin-Producing Escherichia coli Isolated from Goats in Southern Thailand. Tropical Medicine and Infectious Disease. 2022; 7(11):357. https://doi.org/10.3390/tropicalmed7110357
Chicago/Turabian StyleWiriyaprom, Ratchakul, Ruttayaporn Ngasaman, Domechai Kaewnoi, and Sakaoporn Prachantasena. 2022. "Prevalence and Virulent Gene Profiles of Sorbitol Non-Fermenting Shiga Toxin-Producing Escherichia coli Isolated from Goats in Southern Thailand" Tropical Medicine and Infectious Disease 7, no. 11: 357. https://doi.org/10.3390/tropicalmed7110357
APA StyleWiriyaprom, R., Ngasaman, R., Kaewnoi, D., & Prachantasena, S. (2022). Prevalence and Virulent Gene Profiles of Sorbitol Non-Fermenting Shiga Toxin-Producing Escherichia coli Isolated from Goats in Southern Thailand. Tropical Medicine and Infectious Disease, 7(11), 357. https://doi.org/10.3390/tropicalmed7110357